scholarly journals Investigating the Cytotoxic and Anti-Bacterial Activity of Commercially Available Local Anesthetics: An In-Vitro Analysis

2020 ◽  
Vol 29 (04) ◽  
pp. 185-192
Author(s):  
Eisha Imran ◽  
◽  
Faisal Moeen ◽  
Humayoon Satti ◽  
Lubna Rahman
Keyword(s):  
Local Anesthetic ◽  
Local Anesthetics ◽  
Bacterial Activity ◽  
Dilution Method ◽  
Human Mscs ◽  
Bacterial Strains ◽  
Significant Difference ◽  
Vitro Analysis ◽  
In Vitro Analysis

OBJECTIVE: To evaluate and compare the influence of three local anesthetic dental formulations manufactured in France (Septodont), Korea (Medicaine) and Pakistan (HD-Caine) in terms of cytotoxicity and anti-bacterial activities. METHODOLOGY: 90 commercially available local anesthetic cartridges of similar composition (2% lidocaine with epinephrine 100,000) viz. Septodont, Medicaine and HD-Caine were randomly collected from three different Pakistani cities and were assigned as Group S, Group M and Group H, respectively. The cartridges were further divided into three sub-groups each consisting of 10 cartridges to first evaluate cytotoxicity on Mesenchymal Stem Cells (MSCs) using a flow cytometer and secondly to investigate anti-bacterial activity by measuring zones of inhibition and through Broth Dilution Method against five bacterial strains. RESULTS: The results indicated that Septodont (94.5±0.1) and Medicaine (94.7±0.0) showed the highest viability percentage with no significant difference when the two were compared (P=0.6). HD-Caine (93.9±0.0) showed the least, being significantly (P<0.01) different from Septodont and Medicane. A statistically significant (P<0.05) difference was identified between the three study groups regarding the anti-bacterial activity. HD-Caine showed the highest anti-bacterial potential, followed by Medicaine and Septodont. CONCLUSION: Mild toxicity was observed by all the three groups in human MSCs, justifying their safe use in clinical practice. Additionally, Medicane and HD-Caine showed significant anti-bacterial activity indicating their possible use as sterile irrigants. KEYWORDS: Dental anesthesia, Lidocaine, Epinephrine, Antibacterial activity HOW TO CITE: Imran E, Moeen F, Satti H, Rahman L. Investigating the cytotoxic and anti-bacterial activity of commercially available local anesthetics: An in-vitro analysis. J Pak Dent Assoc 2020;29(4):185-192.

scholarly journals Comparative Analysis of Different Local Anesthetic Solutions Available in Market: An In Vitro and Clinical Study

2021 ◽  
Author(s):  
Eisha Imran ◽  
Faisal Moeen ◽  
Beenish Abbas ◽  
Bakhtawar Yaqoob ◽  
Mehreen Wajahat ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Local Anesthetic ◽  
Compositional Analysis ◽  
Bacterial Activity ◽  
Similar Composition ◽  
Ph Values ◽  
Significant Difference ◽  
The Mean ◽  
The Difference

Abstract Objectives The study aimed to evaluate and compare various commercially available local anesthetic solutions. Materials and Methods A total of 150 commercially available local anesthetic cartridges of similar composition (2% lidocaine with epinephrine 1:100,000) were randomly collected and divided into 3 groups. The designations of groups were selected from their product names such that each group consisted of 60 cartridges. Group S (Septodont, France) Group M (Medicaine, Korea) and Group H (HD-Caine, Pakistan). The samples were divided into five sub-groups, each consisting of 10 cartridges from each group to investigate each parameter. Results The acquired data was statistically analyzed and compared (using SPSS version 12). Compositional analysis revealed a non-significant (P>0.05) difference when the three Groups were compared with standard lidocaine and epinephrine solutions. The mean pH values of samples from group S, M and H respectively fell within the range of pH values of commercially available solutions. Non-significant difference in EPT values of Group S and H was found when efficacy was compared (p = 0.3), however a significant difference (p < 0.01) was observed in contrast to Group M. Anti-bacterial activity was observed in all the group and a non-significant difference in cell viability values of Group S and M was found (p = 0.6), while the difference was significant in comparison to Group H. Conclusion Within the limitations of these investigations, it appears that the properties of different manufacturers fall within the recommended ranges as mentioned in literature and do not appear to be statistically different in the variables we have tested.

scholarly journals The Efficacy of Reciproc Blue® For Canal Cleanliness Following the Retreatment of Maxillary First Molar: In Vitro Analysis

2021 ◽  
Vol 7 (1) ◽  
pp. 22-27
Author(s):  
Mohd Nazrin Isa ◽  
Aws Hashim Ali Al-Kadhim ◽  
Azlan Jaafar
Keyword(s):  
Filling Material ◽  
Volume Percentage ◽  
Gutta Percha ◽  
Maxillary Molars ◽  
Significant Difference ◽  
Time Required ◽  
Vitro Analysis ◽  
Cleaning And Disinfection ◽  
In Vitro Analysis

Nonsurgical retreatment involves removing mechanical barriers such as gutta-percha to achieve proper cleaning and disinfection. The complexity of the anatomy of molar tooth gives challenge in retreatment procedure. Thus, this study evaluates the amount of residual gutta-percha after retreatment with rotary files (Reciproc Blue®) from each maxillary first molar canal using cone-beam computed tomography (CBCT) and the time required to accomplish it. Nine freshly extracted maxillary molars were instrumented and obturated. Preoperative CBCT was taken, and retreatment was done using Reciproc Blue®. CBCT was taken post retreatment, and the residual volume percentage of gutta-percha from each canal was calculated. The total retreatment time was recorded, and the data were statistically analyzed. The result shows no statistically significant difference in the amount of residual filling material in mesiobuccal, distobuccal, and palatal canal for maxillary first molar and total time used for retreatment with Reciproc Blue® system.

scholarly journals In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Fermentation ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 100 ◽  
Author(s):  
Gabriela Krausova ◽  
Ivana Hyrslova ◽  
Iveta Hynstova
Keyword(s):  
Lactobacillus Reuteri ◽  
Surface Hydrophobicity ◽  
Animal Origin ◽  
Bacterial Strains ◽  
Mixed Cell ◽  
Infant Feces ◽  
Probiotic Strains ◽  
Vitro Analysis ◽  
In Vitro Analysis

We isolated several strains of human and animal origin, focusing on the genera Lactobacillus and Bifidobacterium. Samples of cow colostrum, feces and saliva of calves and piglets, and infant feces were acquired, bacterial strains were isolated and identified, and twenty strains of lactobacilli and bifidobacteria were shortlisted for further in vitro analysis of adhesion capacity to human cells, surface hydrophobicity, and auto-aggregation. Adhesion capacity was evaluated using a mixture of Caco-2 and HT-29-MTX cells and hydrophobicity was measured using the microbial adhesion to the hydrocarbon method. Lactobacillus reuteri was the most frequently isolated species, followed by L. casei subsp. paracasei and L. gasseri; all strains were isolated from infant feces. Bifidobacteria were represented by the species B. longum (infant feces), and B. thermophilum and B. pseudolongum (calves, piglets). All twenty strains showed good adhesion capacities to the mixed cell culture (17.7–37.2%), particularly, L. reuteri isolates K7 and K14 (37.2% and 35.5%, respectively). In hydrophobicity and auto-aggregation assays, strain-specific differences irrespective of the origin or taxonomic group were observed. Hydrophobicity values varied considerably (from 6.1% to 87.4%), whereas auto-aggregation ability ranged from 21.7% to 69.7%. No relation was observed between hydrophobicity and adhesion capacity; instead, auto-aggregation was apparently related with adhesion.

scholarly journals Trueness of Intraoral Scanners in Implant-Supported Rehabilitations: An In Vitro Analysis on the Effect of Operators’ Experience and Implant Number

2021 ◽  
Vol 10 (24) ◽  
pp. 5917
Author(s):  
Paolo Pesce ◽  
Francesco Bagnasco ◽  
Nicolò Pancini ◽  
Marco Colombo ◽  
Luigi Canullo ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Clinical Results ◽  
Operator Experience ◽  
Clinical Scenarios ◽  
Significant Difference ◽  
Experienced Operator ◽  
Vitro Analysis ◽  
Single Implant ◽  
In Vitro Analysis

(1) Background: Intraoral scanners (IOS) are widely used in prosthodontics. However, a good trueness is mandatory to achieve optimal clinical results. The aim of the present in vitro study was to compare two IOS considering the operator’s experience and different implant clinical scenarios. (2) Methods: Two IOS (IT—Itero, Align Technology; and OS—Opera MC, Opera System, Monaco) were compared simulating three different clinical scenarios: single implant, two implants, and full-arch rehabilitation. Ten scans were taken for each configuration by two different operators (one expert, one inexperienced); influence of operator experience and the type of scanner used was investigated. (3) Results: Trueness of the scans differed between the experienced and non-experienced operator and this difference was statistically significant in all the three scenarios (p = 0.000–0.001, 0.037). A significant difference was present between the scanners (p = 0.000), in the two-implant and full-arch scenarios (p = 0.00). (4) Conclusions: Experience of the operator significantly affect trueness of IT and OP scanners. A statistically significant difference was present among IOS in the two-implant and full-arch scenarios.

scholarly journals In Vitro Analysis of the Erosive Potential of Whey Protein

2020 ◽  
Vol 9 (9) ◽  
pp. e487997742
Author(s):  
Myrelle Leal Campos Sousa ◽  
Isabela Alice Soares de Medeiros ◽  
Liege Helena Freitas Fernandes ◽  
Smyrna Luiza Ximenes de Souza ◽  
Alidianne Fábia Cabral Cavalcanti ◽  
...  
Keyword(s):  
Whey Protein ◽  
Dental Enamel ◽  
Soluble Solids ◽  
Total Soluble Solids ◽  
Significant Difference ◽  
Vitro Analysis ◽  
In Vitro Analysis

The objective of this study was to evaluate the pH and total soluble solids (TSS) content of whey protein beverages. Nine products of three different flavors (vanilla, strawberry and chocolate) of the following brands Muke®, Best Whey®, and Essencial Nutrition Whey®, had their pH evaluated by potentiometry, and their TSS evaluated by refractometry. The lowest pH recorded was for Muke® Chocolate (5.75), and the highest was for Essencial Nutrition Whey® Cacao (6.91). Regarding TSS, the lowest average was for Essencial Nutrition Whey® Vanilla (10.17), and the highest was for Essencial Nutrition Whey® Red Berry (16.66). There was no statistically significant difference in pH and TSS among the flavors and brands evaluated. It was concluded that the analyzed beverages had a higher pH than the pH that is critical to dental enamel and the TSS content was considered high.

scholarly journals Chemical constituents and in-vitro rumen fermentation for dietary inclusion of Delonix regia seed meal for sustainable ruminant production

2020 ◽  
Vol 46 (5) ◽  
Author(s):  
A. A. Lamidi ◽  
J. I. Wilson ◽  
O. O. Joseph
Keyword(s):  
Chemical Constituents ◽  
Gas Production ◽  
Metabolizable Energy ◽  
In Vitro Digestibility ◽  
Delonix Regia ◽  
Significant Difference ◽  
Experimental Treatments ◽  
Vitro Analysis ◽  
In Vitro Analysis

This study was conducted to assess the chemical composition and in-vitro digestibility for dietary inclusion of Delonix regia seeds meal (DSM) for sustainable ruminant production. Four experimental treatments were formulated and DSM was included and varied as T1 (35/0%), T2 (20/15%), T3 (15/20%) and T4 (0/35%), respectively, each treatment were replicated and analyzed for the proximate composition and in-vitro analysis. There were significant differences (P<0.05) in the nutrients and fiber fractions (CP, EE, ash, OM, NFE, NDF, ADF and hemicellulose) across the experimental treatments as CP ranged between 10.50 – 13.60% and decreased with inclusion of DSM. The NFE, NDF and hemicelluloses were similar (P>0.05) in T1 and T4. In-vitro analysis indicated that the methane gas volume was significantly (P<0.05) influenced with the dietary inclusion of DSM across the experimental treatments as T2 had the highest volume of CH (0.61mL) while the least was recorded for T1 (0.47 mL). There was no significant difference (P>0.05) for 24hrs gas production, CH percentage, CH reduction percentage, in-vitro dry matter digestibility (IDMD), in-vitro organic matter digestibility (IOMD), metabolizable energy and fermentation efficiency across the treatments. Conclusively, the nutrients profile of the experimental treatments justifies the utilization of DSM for sustainable ruminant production. Similarity in post in-vitro parameters across treatments is an indication that inclusion of DSM will go a long way to resolve the challenges of feed and feeding for ruminant production.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga
Keyword(s):  
Vitro Analysis ◽  
In Vitro Analysis
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