The Effects of Glycerl Trinitrate and Adenosine 5-Triphosphate on Activation of Potassium Channel-Mediated Vasorelaxation in Female rats aortic Smooth Muscle.

2016 ◽  
Vol 4 (1) ◽  
pp. 1-8
Author(s):  
Omar Al-Habib ◽  
Chinar Mohammed
Keyword(s):  
Smooth Muscle ◽  
Potassium Channel ◽  
Female Rats ◽  
Aortic Smooth Muscle

scholarly journals Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle

2010 ◽  
Vol 57 (3,4) ◽  
pp. 237-244 ◽  
Author(s):  
Takashi Kawano ◽  
Katsuya Tanaka ◽  
Yinhua ◽  
Satoru Eguchi ◽  
Hiroaki Kawano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Potassium Channel ◽  
Cell Line ◽  
Channel Activity ◽  
Aortic Smooth Muscle ◽  
Potassium Channel Activity

Functional reconstitution of the large-conductance, calcium-activated potassium channel purified from bovine aortic smooth muscle

Biochemistry ◽  
1995 ◽  
Vol 34 (48) ◽  
pp. 15849-15862 ◽  
Author(s):  
Kathleen M. Giangiacomo ◽  
Margarita Garcia-Calvo ◽  
Hans-Gunther Knaus ◽  
Theodore J. Mullmann ◽  
Maria L. Garcia ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Potassium Channel ◽  
Aortic Smooth Muscle ◽  
Calcium Activated Potassium Channel

Neutrophil migration through in vitro interstitial matrix

1992 ◽  
Vol 50 (1) ◽  
pp. 894-895
Author(s):  
J. Roemer ◽  
S.R. Simon
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cells ◽  
Inflammatory Cell ◽  
Neutrophil Migration ◽  
Culture Conditions ◽  
Aortic Smooth Muscle ◽  
Specific Culture

We are developing an in vitro interstitial extracellular matrix (ECM) system for study of inflammatory cell migration. Falcon brand Cyclopore membrane inserts of various pore sizes are used as a support substrate for production of ECM by R22 rat aortic smooth muscle cells. Under specific culture conditions these cells produce a highly insoluble matrix consisting of typical interstitial ECM components, i.e.: types I and III collagen, elastin, proteoglycans and fibronectin.

Sorghum Fermented by Aspergillus oryzae NK Enhances Inhibition of Vascular Inflammation in TNF-α-stimulated Human Aortic Smooth Muscle Cells

2018 ◽  
Vol 88 (5-6) ◽  
pp. 309-318
Author(s):  
Hae Seong Song ◽  
Jung-Eun Kwon ◽  
Hyun Jin Baek ◽  
Chang Won Kim ◽  
Hyelin Jeon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Adhesion Molecule ◽  
Vascular Inflammation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Tnf Α ◽  
Cox 2

Abstract. Sorghum bicolor L. Moench is widely grown all over the world for food and feed. The effects of sorghum extracts on general inflammation have been previously studied, but its anti-vascular inflammatory effects are unknown. Therefore, this study investigated the anti-vascular inflammation effects of sorghum extract (SBE) and fermented extract of sorghum (fSBE) on human aortic smooth muscle cells (HASMCs). After the cytotoxicity test of the sorghum extract, a series of experiments were conducted. The inhibition effects of SBE and fSBE on the inflammatory response and adhesion molecule expression were measured using treatment with tumor necrosis factor-α (TNF-α), a crucial promoter for the development of atherosclerotic lesions, on HASMCs. After TNF-α (10 ng/mL) treatment for 2 h, then SBE and fSBE (100 and 200 μg/mL) were applied for 12h. Western blotting analysis showed that the expression of vascular cell adhesion molecule-1 (VCAM-1) (2.4-fold) and cyclooxygenase-2 (COX-2) (6.7-fold) decreased, and heme oxygenase-1 (HO-1) (3.5-fold) increased compared to the TNF-α control when treated with 200 μg/mL fSBE (P<0.05). In addition, the fSBE significantly increased the expression of HO-1 and significantly decreased the expression of VCAM-1 and COX-2 compared to the TNF-α control in mRNA level (P<0.05). These reasons of results might be due to the increased concentrations of procyanidin B1 (about 6-fold) and C1 (about 30-fold) produced through fermentation with Aspergillus oryzae NK for 48 h, at 37 °C. Overall, the results demonstrated that fSBE enhanced the inhibition of the inflammatory response and adherent molecule expression in HASMCs.

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