in Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media

2014 ◽  
Vol 2 (2) ◽  
pp. 310-322
Author(s):  
Layla AL-Mizory ◽  
Ameena Hassan
Keyword(s):  
Culture Media ◽  
In Vitro Multiplication ◽  
Photinia X Fraseri

In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators

2019 ◽  
Vol 15 (1) ◽  
pp. 1-7
Author(s):  
Daniele Camargo Nascimento ◽  
Maximiliano Dini ◽  
Norton Victor Sampaio ◽  
Márcia Wulff Schuch
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
In Vitro Multiplication

scholarly journals In Vitro Multiplication of Potato (Solanum tuberosum L.) cv. Cristal under Different MS Salt and Sucrose Concentrations

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 471E-471
Author(s):  
Gerson R. de L. Fortes ◽  
Luciana B. Andrade ◽  
Marisa de F. Oliveira ◽  
Nilvane T.G. Müller ◽  
Janine T. C. Faria
Keyword(s):  
Culture Media ◽  
Solanum Tuberosum L ◽  
Sucrose Concentration ◽  
Sugar Content ◽  
Potato Cultivar ◽  
In Vitro Multiplication ◽  
Full Strength ◽  
High Dry Matter ◽  
The Media

The potato cultivar Cristal has recently been released by the CPACT/EMBRAPA Breeding Program. Such cultivar was selected for having high dry matter and low sugar content, which makes it desirable for the chip industry. However, this is a recalcitrant cultivar as far as in vitro multiplication is concerned. The aim of this work was to improve the rate of multiplication for this cultivar when it was submitted to different MS salt and sucrose concentrations in the culture media. Two-bud microcuttings were inoculated in test tubes (20 × 150) mm with 10 ml MS media at 3/4-, 1/2-, and full-strength and MS vitamins added to: myo-inositol (100 mg·L–1), agar (7.0 g·L–1) and sucrose as follows: 10, 20 and 30 g·L-1. Each treatment was repeated eight times and each replicate had eight explants. After inoculation the whole material was kept in a growth room at 25 ± 2°C, 16-hr photoperiod and 2000 lux. The evaluation was done 35 days later. It was found and increase in the number of buds as the sucrose concentration in the media decreased. As far as MS salts are concerned no difference in bud number was observed. The rate of multiplication was slightly higher for MS media at full strength and sucrose at low concentration (10 g·L–1). This treatment could be recommended for this cultivar.

scholarly journals In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)

2003 ◽  
Vol 27 (4) ◽  
pp. 421-425 ◽  
Author(s):  
Arildo José Braz de Oliveira ◽  
Vanda Marilza de Carvalho ◽  
Alexandre Ferreira ◽  
Fernando Y. Sato ◽  
Maria de Fátima Pires da Silva Machado
Keyword(s):  
Culture Media ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Favorable Effect ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Short Pulses ◽  
In Vitro Multiplication ◽  
Synthetic Drugs

This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.

scholarly journals In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

2018 ◽  
Vol 18 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Wesley Pires Flausino Máximo ◽  
Paulo Augusto Almeida Santos ◽  
Guilherme Silva Martins ◽  
Evânia Galvão Mendonça ◽  
Luciano Vilela Paiva
Keyword(s):  
Culture Media ◽  
Bioreactor Culture ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Multiplication ◽  
Eucalyptus Hybrid

scholarly journals THE EFFECTS OF THE CULTURE MEDIA BY IN VITRO MULTIPLICATION OF 'Mr. S 25 ' ROOTSTOCK

2017 ◽  
Vol 15 (1) ◽  
pp. 9
Author(s):  
Cibele Merched Gallo
Keyword(s):  
Culture Media ◽  
In Vitro Multiplication

O portaenxerto para Prunus Mr. S. 2/5 não produz sementes, portanto a sua multiplicação só é possível por via assexuada. Para esse tipo de material vegetal, a propagação in vitro pode ser uma estratégia viável para obter alta taxa de multiplicação em curto espaço de tempo. Sendo assim, o presente trabalho buscou identificar a melhor composição salina do meio, pH, fonte de carboidrato e concentração de AIB no meio de cultura, que  proporcionam as melhores taxas de multiplicação in vitro do portaenxerto ‘Mr. S. 2/5’. Este estudo foi dividido em três experimentos, sendo todos conduzidos a partir de explantes com 1 cm, contendo 20 explantes por tratamento, cultivados em sala de crescimento a 25±2<sup>o</sup>C, fotoperíodo de 16 horas e densidade de fluxo luminoso de 48 μmol m-2s-1. As avaliações foram realizadas sempre aos 30 dias após a instalação de cada experimento. Em meio MS com pH 5,2 obteve-se o maior número de brotações por explante. Entretanto, a fonte e a concentração de carboidrato não exerceram influência sobre o número e o comprimento das brotações formadas. Quando o meio foi suplementado com 0,06 mg L<sup>-1</sup> de AIB se registrou a formação do maior número de brotações, porém sem influência significativa no comprimento das mesmas.

scholarly journals In vitro multiplication of pear tree cultivar Cascatense

2016 ◽  
Vol 37 (2) ◽  
pp. 581 ◽  
Author(s):  
Ilda Mariclei De Castro da Silva ◽  
Cristina Copstein Cuchiara ◽  
Mara Cíntia Winhelmann ◽  
Valmor João Bianchi ◽  
Eugenia Jacira Bolacel Braga ◽  
...  
Keyword(s):  
Culture Media ◽  
Dry Mass ◽  
High Yield ◽  
Temperate Climate ◽  
In Vitro Multiplication ◽  
Pear Tree ◽  
In Vitro Micropropagation ◽  
Sequential Experiments ◽  
Cold Requirement

One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose.

In vitro multiplication of different B. rapa genotypes using different tissue culture media

2012 ◽  
Vol 29 ◽  
pp. S140
Author(s):  
Emine Aydin ◽  
Ferda Altay ◽  
Mehmet Can ◽  
Fatih Seyis
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
In Vitro Multiplication ◽  
Tissue Culture Media

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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