158 Lactobacillus acidophilus fermentation product modulates inflammatory activity by regulating the TLR4 and NFkB expression in porcine peripheral blood mononuclear cells after lipopolysaccharide challenge

2016 ◽  
Vol 94 (suppl_2) ◽  
pp. 74-74
Author(s):  
S. I. Lee ◽  
J. M. Koo ◽  
R. X. Lan ◽  
I. H. Kim
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Lactobacillus Acidophilus ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Inflammatory Activity ◽  
Peripheral Blood Mononuclear ◽  
Fermentation Product ◽  
Blood Mononuclear Cells ◽  
Lipopolysaccharide Challenge

scholarly journals Lactobacillus acidophilus modulates inflammatory activity by regulating the TLR4 and NF-κB expression in porcine peripheral blood mononuclear cells after lipopolysaccharide challenge

2016 ◽  
Vol 115 (4) ◽  
pp. 567-575 ◽  
Author(s):  
Sang In Lee ◽  
Hyun Soo Kim ◽  
Jin Mo Koo ◽  
In Ho Kim
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Lactobacillus Acidophilus ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Control Diet ◽  
Inflammatory Activity ◽  
Daily Weight Gain ◽  
Peripheral Blood Mononuclear ◽  
Lps Challenge ◽  
Blood Mononuclear Cells

AbstractA total of forty weaned pigs ((Landrace×Yorkshire)×Duroc) were used to evaluate the effects of Lactobacillus acidophilus on inflammatory activity after lipopolysaccharide (LPS) challenge. Experimental treatments were as follows: (T1) control diet+saline challenge; (T2) control diet with 0·1 % L. acidophilus+saline challenge; (T3) control diet+LPS challenge; and (T4) control diet with 0·1 % L. acidophilus+LPS challenge. On d-14, piglets were challenged with saline (T1 and T2) or LPS (T3 and T4). Blood samples were obtained at 0, 2, 4, 6 and 12 h after being challenged and analysed for immune cell cytokine production and gene expression pattern. The L. acidophilus treatment increased the average daily weight gain (ADWG) and average daily feed intake (ADFI) compared with the control diet. With the control diet, the LPS challenge (T3) increased the number of immune cells and expression of TNF-α and IL-6 compared with the saline challenge (T1). Whereas with the saline challenge L. acidophilus treatment (T2) increased the number of leucocytes and CD4 compared with the control diet (T1), with the LPS challenge L. acidophilus treatment (T4) decreased the number of leucocytes, lymphocytes, CD4+ and CD8+ and expression of TNF-α and IL-6 compared with the control diet (T3). L. acidophilus treatment decreased the expression of TRL4 and NF-κB in peripheral blood mononuclear cells (PBMC) after LPS challenge, which leads to inhibition of TNF-α, IFN-γ, IL-6, IL-8 and IL1B1 and to induction of IL-4 and IL-10. We suggested that L. acidophilus improved ADWG and ADFI and protected against LPS-induced inflammatory responses by regulating TLR4 and NF-κB expression in porcine PBMC.

In vitro anti inflammatory activity of Aloe vera by down regulation of MMP-9 in peripheral blood mononuclear cells

2012 ◽  
Vol 141 (1) ◽  
pp. 542-546 ◽  
Author(s):  
Damodharan Vijayalakshmi ◽  
Ramamurthy Dhandapani ◽  
Sivalingam Jayaveni ◽  
Panneer Selvam Jithendra ◽  
Chellan Rose ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Aloe Vera ◽  
Inflammatory Activity ◽  
Down Regulation ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Anti Inflammatory
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