scholarly journals Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, (Risso, 1810)

2017 ◽  
Vol 95 (9) ◽  
pp. 4085 ◽  
Author(s):  
R. Zupa ◽  
C. Fauvel ◽  
C. C. Mylonas ◽  
C. Pousis ◽  
N. Santamaria ◽  
...  
Keyword(s):  
Sperm Quality ◽  
In Captivity ◽  
Greater Amberjack

scholarly journals Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

2017 ◽  
Vol 95 (9) ◽  
pp. 4085-4100 ◽  
Author(s):  
R. Zupa ◽  
C. Fauvel ◽  
C. C. Mylonas ◽  
C. Pousis ◽  
N. Santamaria ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Seriola Dumerili ◽  
In Captivity ◽  
Greater Amberjack

scholarly journals Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

2016 ◽  
Vol 37 (4) ◽  
pp. 2167 ◽  
Author(s):  
Mayara Setúbal Oliveira ◽  
Priscila Silva de Almeida-Monteiro ◽  
Larissa Teixeira Nunes ◽  
Francisco Renan Aragão Linhares ◽  
João Paulo Silva Pinheiro ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Dilution Rate ◽  
Freshwater Fish ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Motile Sperm ◽  
Colossoma Macropomum ◽  
Freezing Method ◽  
In Captivity ◽  
Fresh Semen

Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen.

scholarly journals Sex differentiation and early sex identification in hatchery produced greater amberjack (Seriola dumerili) reared in sea cages

2021 ◽  
Author(s):  
Maria Papadaki ◽  
Manolis Mandalakis ◽  
Thekla I. Anastasiou ◽  
Marina Pouli ◽  
Michalis Asderis ◽  
...  
Keyword(s):  
Sex Differentiation ◽  
Plasma Concentrations ◽  
Differentiation Process ◽  
Mean Values ◽  
Seriola Dumerili ◽  
In Captivity ◽  
Liquid Chromatography Tandem Mass ◽  
Greater Amberjack ◽  
Males And Females ◽  
The Mean

Abstract The histological process of gonadal differentiation together with the endocrine changes of glucocorticoids and sex steroids was studied in hatchery produced greater amberjack (Seriola dumerili) from 101 until 408 days post-hatching. In the samplings, which were conducted every 50 days, no size dimorphism was observed between the sexes and sex ratio was 1:1, suggesting that there was no influence of early rearing in captivity on sex differentiation. Plasma concentrations of cortisol, cortisone, adrenosterone (Ad), androstenedione (Δ4), 11-ketotestosterone (11ΚΤ), testosterone (Τ), estradiol (Ε2), progesterone (P4) and 17,20β-dihydroxyprogesterone (DHP) were measured in males and females with the use of liquid chromatography tandem mass spectrometry (LC-MS/MS) to examine their role in the sex differentiation process. From the nine hormones, the only one that exhibited differences between the sexes was 11-KT. However, variations were observed in the mean values of cortisol, Ad, Δ4, 11-KT, T, P4 and DHP over time in one or both sexes, indicating their involvement in the sex differentiation process. Moreover, the 11-KT/E2 ratio was found to be a useful diagnostic biomarker for greater amberjack sex, from as early as 150 dph.

scholarly journals Development of Sperm Cryopreservation Protocols for Sharks and Rays: New Tools for Elasmobranch Conservation

2021 ◽  
Vol 8 ◽  
Author(s):  
Pablo García-Salinas ◽  
Victor Gallego ◽  
Juan F. Asturiano
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Sperm Cryopreservation ◽  
In Captivity ◽  
Reproductive Techniques ◽  
Elasmobranch Species ◽  
Reproductive Cycles

Elasmobranchs are one of the most endangered vertebrate groups on the planet, but despite this situation the use of reproductive techniques in elasmobranch conservation strategies has been scarce. Among these techniques, sperm preservation is a potential tool for ex situ conservation and aquaria sustainability. However, there are no widespread preservation protocols for elasmobranch sperm, and shark sperm cryopreservation has never been achieved before. Here we present the establishment of successful cryopreservation protocols for elasmobranch sperm, tested in several species. We have formulated a sperm extender that can be used for different elasmobranch species, capable of maintaining sperm motility for several weeks. Additionally, we achieved the cryopreservation of sperm by previously diluting it in our extender and supplementing it with different combinations of cryoprotectants. The effects of methanol and dimethyl sulfoxide as permeating cryoprotectants were evaluated, as well egg yolk as a non-permeating cryoprotectant. Sperm quality was assessed by studying the motility and membrane integrity post-thawing, demonstrating its effectiveness in the 10 species tested, including two which are considered Critically Endangered. This is the first time that shark sperm cryopreservation has been reported, broadening our knowledge of the reproductive techniques that can be applied to elasmobranchs and laying the foundations for the first cryobanks for shark and ray sperm. Outcomes from this study will be useful for ex situ conservation efforts developed by public aquaria. A regular supply of frozen sperm will reduce the problems that result from the transport of specimens, inbreeding or lack of synchronized reproductive cycles in captivity.

The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin

2017 ◽  
Vol 49 (1) ◽  
pp. 243-252 ◽  
Author(s):  
Chrysovalentinos Pousis ◽  
Constantinos C Mylonas ◽  
Caterina De Virgilio ◽  
Gemma Gadaleta ◽  
Nicoletta Santamaria ◽  
...  
Keyword(s):  
Seriola Dumerili ◽  
In Captivity ◽  
Greater Amberjack

Natural spawning of greater amberjack (Seriola dumerili) kept in captivity in the Canary Islands

Aquaculture ◽  
2006 ◽  
Vol 252 (2-4) ◽  
pp. 199-207 ◽  
Author(s):  
S. Jerez ◽  
M. Samper ◽  
F.J. Santamaría ◽  
J.E. Villamandos ◽  
J.R. Cejas ◽  
...  
Keyword(s):  
Canary Islands ◽  
Seriola Dumerili ◽  
Natural Spawning ◽  
In Captivity ◽  
Greater Amberjack

Male reproductive dysfunction in Solea senegalensis: new insights into an unsolved question

2019 ◽  
Vol 31 (6) ◽  
pp. 1104 ◽  
Author(s):  
Marta F. Riesco ◽  
David G. Valcarce ◽  
Juan Manuel Martínez-Vázquez ◽  
Ignacio Martín ◽  
Andrés Ángel Calderón-García ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Molecular Signature ◽  
Solea Senegalensis ◽  
Reproductive Dysfunction ◽  
Sex Preferences ◽  
Dopaminergic Pathway ◽  
Sustainable Culture ◽  
In Captivity ◽  
In The Wild ◽  
Post Transcriptional Regulation

Senegalese sole (Solea senegalensis) is a species with a high commercial value that exhibits a reproductive dysfunction in males born and raised in captivity (F1) that hinders their sustainable culture. The present study evaluates the sperm quality and dopaminergic pathway of males born in the wild environment and of F1 males. Traditional sperm analyses were performed, finding only significant differences in curvilinear velocity (VCL) and no significant differences in viability and total motility. No differences in global sperm methylation were observed either in spermatozoa or brain between the two groups (F1 and wild-born males). However, our results point to a different sperm molecular signature between wild fish and fish born in captivity, specifically the differential expression in miR-let7-d and miR-200a-5p between these two groups. miR-let7-d has been correlated with spermatogenesis and sex preferences, whereas the miR-200 family is implied in target innervation of dopaminergic neurons in zebrafish. When we analysed the dopaminergic pathway, no differences were found in terms of different mRNA expression of dopaminergic markers. However, some differences were detected in terms of tyrosine hydroxylase protein expression by western blot analysis, thus suggesting an altered post-transcriptional regulation in F1 males. The results of this study suggest that an altered sperm miRNA signature in F1 males could be one possible mode of transmission of reproductive dysfunction to the progeny.

Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season and captivity

2007 ◽  
Vol 19 (2) ◽  
pp. 370 ◽  
Author(s):  
Adrienne E. Crosier ◽  
Laurie Marker ◽  
JoGayle Howard ◽  
Budhan S. Pukazhenthi ◽  
Josephine N. Henghali ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Ex Situ ◽  
Free Living ◽  
Acinonyx Jubatus ◽  
Young Males ◽  
Sperm Density ◽  
In Captivity ◽  
Free Ranging

The objective was to examine the influence of animal age, season and captivity status on seminal quality in wild-born cheetahs (Acinonyx jubatus) in Namibia, Africa. Animals were divided into three age categories: juvenile (14–24 months; n = 16 males, 23 ejaculates); adult (25–120 months; n = 76 males, 172 ejaculates); and aged (>120 months; n = 5 males, 5 ejaculates). Seasons were categorised into hot–wet (January–April), cold–dry (May–August) and hot–dry (September–December). A comparison between freshly wild-caught (n = 29 males, 41 ejaculates) and captive-held cheetahs (n = 68 males, 159 ejaculates) was also conducted. Raw ejaculates contained 69.0 ± 1.1% motile spermatozoa (mean ± s.e.m.) with 73.6 ± 1.5% of these cells containing an intact acrosome. Overall, 18.4 ± 0.9% of spermatozoa were morphologically normal, with midpiece anomalies being the most prevalent (~39%) defect. Juvenile cheetahs produced ejaculates with poorer sperm motility, forward progressive status, lower seminal volume and fewer total motile spermatozoa than adult and aged animals. Spermatogenesis continued unabated throughout the year and was minimally influenced by season. Proportions of sperm malformations were also not affected by season. Ejaculates from captive cheetahs had increased volume and intact acrosomes, but lower sperm density than wild-caught counterparts. In summary, Namibian cheetahs produce an extraordinarily high proportion of pleiomorphic spermatozoa regardless of age, season or living (captive versus free-ranging) status. Young males less than 2 years of age produce poorer ejaculate quality than adult and aged males. Because (1) all study animals were wild born and (2) there was little difference between freshly caught males and those maintained in captivity for protracted periods, our results affirm that teratospermia in the cheetah is mostly genetically derived. It also appears that an ex situ environment for the Namibian cheetah can ensure sperm quality comparable with that for free-living males.

Multiple GnRHa injections to induce successful spawning of wild caught greater amberjack (Seriola dumerili) matured in captivity

2013 ◽  
Vol 46 (7) ◽  
pp. 1748-1759 ◽  
Author(s):  
Hipólito Fernández-Palacios ◽  
Dominique Schuchardt ◽  
Javier Roo ◽  
Carmen María Hernández-Cruz ◽  
Marisol Izquierdo
Keyword(s):  
Seriola Dumerili ◽  
In Captivity ◽  
Greater Amberjack
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