P4016 Paternal genetic characterization of wild boars and domestic pigs in Japan, based on SRY and TSPY gene haplotypes

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 86-86
Author(s):  
Y. Sato ◽  
K. Sato ◽  
Y. Mizoguchi
Keyword(s):  
Genetic Characterization ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Tspy Gene

Genetic characterization of porcine circovirus type 2 in captive wild boars in southern Brazil

2017 ◽  
Vol 49 (5) ◽  
pp. 1071-1075 ◽  
Author(s):  
José Paulo Hiroji Sato ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Maurício Egídio Cantão ◽  
Janice Reis Ciacci-Zanella ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Southern Brazil ◽  
Wild Boars

Detection and genetic characterization of a novel parvovirus distantly related to human bufavirus in domestic pigs

2016 ◽  
Vol 161 (4) ◽  
pp. 1033-1037 ◽  
Author(s):  
Renáta Hargitai ◽  
Péter Pankovics ◽  
Attila Mihály Kertész ◽  
Hunor Bíró ◽  
Ákos Boros ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Domestic Pigs

Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars

2005 ◽  
Vol 151 (3) ◽  
pp. 495-507 ◽  
Author(s):  
A. Cságola ◽  
S. Kecskeméti ◽  
G. Kardos ◽  
I. Kiss ◽  
T. Tuboly
Keyword(s):  
Genetic Characterization ◽  
Wild Boars ◽  
Porcine Circoviruses

scholarly journals Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia

2020 ◽  
Vol 132 ◽  
pp. 404-406
Author(s):  
Vesna Milićević ◽  
Branislav Kureljušić ◽  
Jelena Maksimović-Zorić ◽  
Božidar Savić ◽  
Ljiljana Spalević ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Kobuvirus ◽  
Wild Boars ◽  
Domestic Pigs

scholarly journals Genetic Characterization of Porcine Circovirus 3 Strains Circulating in Sardinian Pigs and Wild Boars

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 344
Author(s):  
Silvia Dei Giudici ◽  
Giulia Franzoni ◽  
Piero Bonelli ◽  
Pier Paolo Angioi ◽  
Susanna Zinellu ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Haplotype Network ◽  
Porcine Circovirus ◽  
Nonsynonymous Mutation ◽  
Tissue Samples ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Porcine Circovirus 3 ◽  
Free Ranging

Porcine circovirus 3 (PCV3) is a recently discovered member of the Circoviridae family. So far, its presence has been reported in North America, Asia, South America, and Europe. In this study, blood and tissue samples from 189 Sardinian suids (34 domestic pigs, 115 feral free ranging pigs, and 39 wild boars) were used to genetically characterize the PCV3 strains from Sardinia. PCV3 infection in the animals was confirmed by real time PCR. The detection rate in the three groups analyzed was l7.64% in domestic pigs, 77.39% in free ranging pigs, and 61.54% in wild boars. Moreover, our results showed that co-infection of PCV3 with other viruses is quite a common occurrence. Molecular characterization of Sardinian PCV3 strains was performed by sequencing 6 complete genomes and 12 complete cap genes. Our results revealed that there is a high similarity between our strains and those identified in different countries, confirming the genetic stability of PCV3 regardless of geographical origin. Haplotype network analysis revealed the presence of 6 whole genomes or 12 unique ORF2 haplotypes and a nonsynonymous mutation in ORF2 that leads to an R14K amino acid substitution. Phylogenetic analysis of whole genome and ORF2 was also conducted. The Sardinian strains were allocated in three different clusters of phylogenetic trees of both complete genome and ORF2. With this study, we have provided a snapshot of PCV3 circulation in Sardinia. Our findings might help to achieve a deeper understanding of this emerging porcine virus.

Genetic characterization of African swine fever virus from domestic pigs in India

2021 ◽  
Author(s):  
Katherukamem Rajukumar ◽  
Dhanapal Senthilkumar ◽  
Govindarajulu Venkatesh ◽  
Fateh Singh ◽  
Vishnu P. Patil ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals DETECTION AND GENETIC CHARACTERIZATION OF PORCINE CIRCOVIRUS 2 ISOLATES FROM THE FIRST CASES OF POSTWEANING MULTISYSTEMIC AND WASTING SYNDROME IN WILD BOARS IN GREECE

2008 ◽  
Vol 44 (4) ◽  
pp. 864-870 ◽  
Author(s):  
Marina Sofia ◽  
Charalambos Billinis ◽  
Vassilios Psychas ◽  
Periklis Birtsas ◽  
George Sofianidis ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Porcine Circovirus ◽  
Wild Boars ◽  
Wasting Syndrome ◽  
Porcine Circovirus 2

scholarly journals Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Rizka Hasanah ◽  
UUS SAEPULOH ◽  
DYAH PERWITASARI-FARAJALLAH ◽  
WALBERTO SINAGA ◽  
YOHANA TRI HASTUTI ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Pcr Amplification ◽  
Dimensional Structure ◽  
Illegal Hunting ◽  
West Sumatra ◽  
D Loop ◽  
Mentawai Island ◽  
Tspy Gene ◽  
Hylobates Klossii

Abstract. Hasanah R, Saepuloh U, Perwitasari-Farajallah D, Sinaga W, Hastuti Y, Sajuthi D. 2020. Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia. Biodiversitas 21: 2854-2861. Hylobates klossii is an endemic primate on the Mentawai Island, Indonesia that categorized as endangered species due to fragmentation, illegal hunting, and habitat loss. This study aimed to analyze the genetic characterization of H. klossii based on maternal and paternal lineages. The samples were collected from TSI (Bogor, Indonesia) cage consist of two males and two females (blood and fecal of four individuals) and Siberut Island, Mentawai, West Sumatra, Indonesia from one unknown gender (one individual fecal sample). PCR amplification was carried out on DNA and cDNA samples using specific primers for D-loop MtDNA and TSPY genes. The amplicon was analyzed with MEGA-7. The dimensional structure model of the TSPY protein was determined using I-TASSER and visualized with PyMol. The maternal analysis showed five samples consisted of four haplotypes revealed that Nam-nam and April were identic with 0% genetic distance. It’s revealed that Nam-nam and April were mother and son. The paternal analysis of two H. klossii males revealed that April was the closest relative to H. muelleri (99.56% identity) and Gou-gou to H. klossii (100% identity). Characterization of partial TSPY gene (990 bp) of April and Gou-Gou indicated four amino acids mutation, causing the shift in-amino acid structure position. The results assumed that April was a cross-breeding between H. klossii female and H. muelleri male. This genetic characterization is expected to support the conservation efforts through recommendations for good management in H. klossii preservation.

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