The use of oxidative stress biomarkers in live animals (in vivo) to predict meat quality deterioration postmortem (in vitro) caused by changes in muscle biochemical components

2017 ◽  
Vol 95 (7) ◽  
pp. 3012
Author(s):  
E. N. Ponnampalam ◽  
D. L. Hopkins ◽  
K. Giri ◽  
J. L. Jacobs ◽  
T. Plozza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Meat Quality ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Quality Deterioration ◽  
Biochemical Components

scholarly journals The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alireza Rezaeinezhad ◽  
Pegah Eslami ◽  
Hossein Mirmiranpour ◽  
Hamid Ghomi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Fluorescence Analysis ◽  
Atmospheric Plasma ◽  
Oxidative Stress Biomarkers ◽  
Cold Atmospheric Plasma ◽  
Stress Biomarkers

AbstractCold atmospheric plasma (CAP) is known as the versatile tool in different biological, and medical applications. In this study, we investigated the effect of cold plasma on diabetes via in vitro and in vivo assessments. We performed the in vitro assay to evaluate the impact of CAP on glycated glutathione peroxidase (GPx) through enzyme activity measurement as a function index and far- and near-UV circular dichroism (CD) and fluorescence analysis as structure indices. The result of in vitro assessment showed that the exposure of glycated GPx to plasma causes a considerable increase in enzyme activity up to 30%. Also, the evaluation of far- and near-UV CD and fluorescence analysis indicated a modification in the protein structure. According to obtained result from in vitro assessment, in vivo assay evaluated the effect of CAP on diabetic mice through analyzing of blood glucose level (BGL), advanced glycation end products (AGEs), antioxidant activity, oxidative stress biomarkers such as malondialdehyde (MDA), advanced oxidation protein products (AOPP), and oxidized low-density lipoprotein (oxLDL), and inflammation factors including tumor necrosis factor (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6). The result of in vivo experiment also showed a 20% increase in antioxidant activity. Also, the reduction in AGEs, oxidative stress biomarkers, and inflammatory cytokines concentrations was observed. The result of this study revealed that CAP could be useful in diabetes treatment and can be utilized as a complementary method for diabetes therapy.

scholarly journals The Neuroprotective Effects of Carvacrol on Ethanol-Induced Hippocampal Neurons Impairment via the Antioxidative and Antiapoptotic Pathways

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Peng Wang ◽  
Qian Luo ◽  
Hui Qiao ◽  
Hui Ding ◽  
Yonggang Cao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Western Blot ◽  
Cognitive Dysfunction ◽  
Hippocampal Neurons ◽  
Morris Water Maze Test ◽  
Oxidative Stress Biomarkers ◽  
Neuroprotective Effects ◽  
Stress Injury

Chronic alcohol consumption causes hippocampal neuronal impairment, which is associated with oxidative stress and apoptosis. Carvacrol is a major monoterpenic phenol found in essential oils from the family Labiatae and has antioxidative stress and antiapoptosis actions. However, the protective effects of carvacrol in ethanol-induced hippocampal neuronal impairment have not been fully understood. We explored the neuroprotective effects of carvacrol in vivo and in vitro. Male C57BL/6 mice were exposed to 35% ethanol for 4 weeks to establish ethanol model in vivo, and hippocampal neuron injury was simulated by 200 mM ethanol in vitro. Morris water maze test was performed to evaluate the cognitive dysfunction. The oxidative stress injury of hippocampal neurons was evaluated by measuring the levels of oxidative stress biomarkers. Histopathological examinations and western blot were performed to evaluate the apoptosis of neurons. The results showed that carvacrol attenuates the cognitive dysfunction, oxidative stress, and apoptosis of the mice treated with ethanol and decreases hippocampal neurons apoptosis induced by ethanol in vitro. In addition, western blot analysis revealed that carvacrol modulates the protein expression of Bcl-2, Bax, caspase-3, and p-ERK, without influence of p-JNK and p-p38. Our results suggest that carvacrol alleviates ethanol-mediated hippocampal neuronal impairment by antioxidative and antiapoptotic effects.

Comparison of grain-based diet supplemented with synthetic vitamin E and lucerne hay-based diet on blood oxidative stress biomarkers and lamb meat quality

2019 ◽  
Vol 177 ◽  
pp. 146-152
Author(s):  
Benjamin W.B. Holman ◽  
Gianluca Baldi ◽  
Surinder S. Chauhan ◽  
David L. Hopkins ◽  
Greg R. Seymour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Meat Quality ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Synthetic Vitamin ◽  
Lamb Meat

Oxidative stress biomarkers in newborn calves: Comparison among artificial insemination, in vitro fertilization and cloning

2020 ◽  
Vol 219 ◽  
pp. 106538 ◽  
Author(s):  
Gabriela N. Dantas ◽  
Bianca P. Santarosa ◽  
Vitor H. Santos ◽  
Henrique B. Hooper ◽  
Ricardo A. Micai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Artificial Insemination ◽  
Oxidative Stress Biomarkers ◽  
Vitro Fertilization ◽  
Stress Biomarkers ◽  
Newborn Calves
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