Technical note: Evaluation of urinary purine derivatives in comparison with duodenal purines for estimating rumen microbial protein supply in sheep

2017 ◽  
Vol 95 (2) ◽  
pp. 884
Author(s):  
G. V. Kozloski ◽  
C. M. Stefanello ◽  
L. Oliveira ◽  
H. M. N. Ribeiro Filho ◽  
T. J. Klopfenstein
Keyword(s):  
Technical Note ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Protein Supply

Technical note: Evaluation of urinary purine derivatives in comparison with duodenal purines for estimating rumen microbial protein supply in sheep1

2017 ◽  
Vol 95 (2) ◽  
pp. 884-891 ◽  
Author(s):  
G. V. Kozloski ◽  
C. M. Stefanello ◽  
L. Oliveira ◽  
H. M. N. Ribeiro Filho ◽  
T. J. Klopfenstein
Keyword(s):  
Technical Note ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Protein Supply

Purine derivatives in spot urine samples and plasma of sheep: a possible index of microbial protein supply

1993 ◽  
Vol 1993 ◽  
pp. 5-5 ◽  
Author(s):  
X. B. Chen ◽  
Adriana T. Mejia ◽  
D. J. Kyle ◽  
E. R. Ørskov
Keyword(s):  
Urinary Excretion ◽  
Feed Intake ◽  
Urine Samples ◽  
Plasma Samples ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Feeding Regime ◽  
Spot Urine ◽  
Alternative Index ◽  
Protein Supply

In ruminants, daily urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply (Chen, Ørskov and Hovell, 1991). The application could be extended to farm conditions if measurements based on spot urine samples or plasma could serve as an alternative index. The objective of this study was to examine whether PD concentrations in spot urine or plasma samples vary diurnally during a given feeding regime and if they reflect differences in daily PD excretion induced by varying feed intake.

scholarly journals PSI-27 Excretion of purine derivatives and rumen microbial protein synthesis in Nelore steers supplemented with low-moisture, sugarcane molasses-based block and fed low-quality forage

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 249-250
Author(s):  
Andresa L Feliciano ◽  
Sérgio A G Pereira-Junior ◽  
Yury Granja-Salcedo ◽  
Livia Maria Sampaio Ferraz Sepini de Souza Grilo ◽  
Luís Felipe Arelaro Artioli ◽  
...  
Keyword(s):  
Random Effect ◽  
Latin Square ◽  
Experimental Period ◽  
Sugarcane Molasses ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Microbial Nitrogen ◽  
Protein Supply ◽  
Protein Block ◽  
Corn Grain

Abstract The objective of this study was to determine the effects of supplementation of low-moisture, sugarcane molasses-based block (LMB) on steers fed low quality forage in the excretion of purine derivatives and in the synthesis of ruminal microbial protein. Six rumen cannulated Nellore steers steers (23 months, 350 ± 10 kg) were distributed in a 3 × 3 double Latin square design. The treatments were composed of Brachiaria brizantha ‘Marandu’ hay ad libtum as an exclusive source of bulky (93.65% DM, 3.97% CP and 81.76% NDF) and supplements: complete mineral blend with urea [UR, (urea, salt, mineral-vitamin premix)], a commercial protein supplement [PS, (corn grain, soybean meal, urea, salt and mineral-vitamin premix)] or low-moisture, cooked sugarcane molasses-based protein block [LMB, (cane molasses, cottonseed meal, soybean oil, urea, salt and mineral-vitamin premix)]. Each experimental period lasted 21 days (14 days of adaptation and 7 days of data collection). The total urinary volume was measured for five days in each experimental period. The urine was collected in rubber funnels fixed by elastic loops on the backs of the animals. The urine was conduct through hoses connected to a 20 L polyethylene bucket containing 250 mL of 20% H2SO4 solution. Every 24 hours, the collected urine was homogenized and the total excreted volume was measured. The data were analyzed using Software R, having as fixed effect the treatments and as animal random effect, period, Latin square and error. Supplementation with LMB lead to greater excretion of allantoin (P = 0.046), microbial nitrogen flow (P = 0.023) and higher microbial crude protein (P = 0.023) into the intestine compared to UR and PS. While no effect was observed on total purines and purines absorbed (P > 0.05). Thus, LMB supplementation for rumen cannulated Nellore steers fed low quality forage was effective to improve metabolizable protein supply.

Measurement of microbial protein synthesis in Iranian buffalo rumen (Mazandran Province) by purine derivatives excretion method

2007 ◽  
Vol 2007 ◽  
pp. 216-216
Author(s):  
Yashar Vakil Faraji ◽  
Mojtaba Zahedifar ◽  
Jafari Khorshidi Kaveh
Keyword(s):  
Protein Synthesis ◽  
Uric Acid ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Mazandaran Province ◽  
Rumen Microbes ◽  
Microbial Protein Synthesis ◽  
North Of Iran ◽  
Swamp Buffalo ◽  
Protein Supply

Rumen microbes are rich in nucleic acid: around 18% of total nitrogen is present on nucleic acids or 11% in purines. Rumen microbes constitue the major source of protein supply to the ruminant. The purines from the rumen microbes are metabolized and excreted in the urine as their end products: hypoxanthine, xanthine, uric acid and allantoin. In buffalo and cattle because of high xanthine oxidase activity in intestine and blood, hypoxanthine and xanthine convert to uric acid therefore only uric acid and allantoin excreted in urine way (Chen, X. B., Ørskov, E. R., 2003). This research carried out to use excretion of purine derivatives namely allantoin and uric acid as a parameter to estimate the microbial protein synthesis in the rumen of native swamp buffalo in north of iran, Mazandaran Province.

Comparison of methods for the measurement of microbial protein supply in sheep

1997 ◽  
Vol 1997 ◽  
pp. 125-125
Author(s):  
M.A. Samaniego ◽  
X.B. Chen ◽  
F.DeB. Hovell ◽  
J.C. MacRae ◽  
R. W. Mayes
Keyword(s):  
Surgical Intervention ◽  
Dual Phase ◽  
Comparison Of Methods ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Flow Rates ◽  
N Supply ◽  
Microbial Nitrogen ◽  
Protein Supply ◽  
Microbial N

The excretion of purine derivatives (PD) in urine has been used as an index for the estimation of microbial nitrogen (N) supply in ruminants. This method is simple and does not require surgical intervention of the animal. However, there have been few attempts to compare the results of this method with other methods for the measurement of microbial N supply. In the present study, two experiments were performed. The main experiment (Experiment 2) compared the estimated duodenal microbial N flow based on urinary PD excretion, with the more direct measurement based on duodenal RNA flow rates obtained from cannulated sheep using n-alkanes as the digesta flow marker. However, a preliminary experiment (Experiment 1) was needed to validate the use of alkane as a digesta marker in comparison with the commonly accepted dual phase marker method of Faichney (1975).

Urinary Excretion of Purine Derivatives in Fed and Fasted Yaks (Bos gruniens)

1998 ◽  
Vol 1998 ◽  
pp. 134-134
Author(s):  
R. J. Long ◽  
X. B. Chen ◽  
S. K. Dong ◽  
D. G. Zhang ◽  
E. R. Ørskov ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Urinary Excretion ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Ruminant Species ◽  
Feeding Experiments ◽  
Protein Supply ◽  
Different Levels

Previous studies in sheep and cattle have demonstrated that the urinary excretion of purine derivatives (PD) provides an index of the intestinal flow of microbial protein (Chen et al. 1990). Yak is the most important ruminant species in Tibetan Plateau. It was of interest to establish whether the approach of PD excretion to estimate microbial protein supply is applicable in yaks. The objective of this work is to measure the effect of fasting and different levels of feeding on PD excretion in yaks.The feeding experiments were carried out in Xi-ning, China at an altitude of 3100m and a latitude of 36.8° N. Six 5-year old female yaks were used and 3 experiments were completed.

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