scholarly journals RAPID COMMUNICATION: Nerve growth factor influences cleavage rate and embryo development in sheep1,2

2016 ◽  
Vol 94 (10) ◽  
pp. 4447-4451 ◽  
Author(s):  
M. Crispo ◽  
P. C. dos Santos-Neto ◽  
M. Vilariño ◽  
A. P. Mulet ◽  
A. de León ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Embryo Development ◽  
Cleavage Rate ◽  
Nerve Growth ◽  
Rapid Communication

117 Supplementation of IVF medium with nerve growth factor improved bovine embryonic cleavage rates during summer months

2020 ◽  
Vol 32 (2) ◽  
pp. 185
Author(s):  
E. Amiss ◽  
J. W. Stewart ◽  
V. M. Negrón-Pérez ◽  
K. Jones ◽  
H. Haines ◽  
...  
Keyword(s):  
Heat Stress ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Embryo Development ◽  
Subsequent Development ◽  
Cumulus Cells ◽  
Cleavage Rate ◽  
Nerve Growth ◽  
Oocyte Competence ◽  
Embryonic Cleavage

Nerve growth factor-β (NGF), a protein originally associated with regulation of neuron development, has been found to play a role in the reproductive system of mammals. Previous research showed that administration of NGF to cows resulted in enhanced conceptus development. Although these effects were speculated to be a result of improved corpus luteum function, whether NGF could act directly on the embryo remained undetermined. Therefore, the direct effects of NGF on fertilization and embryo development warrant investigation to see whether it can be used as a novel tool to improve cleavage and blastocyst rates when producing embryos via IVF during periods of suboptimal oocyte quality, such as with heat stress. The objective of this study was to explore how supplementation of NGF, purified from bull seminal plasma, during IVF may directly affect embryo development in oocytes harvested in the summer. Abattoir-derived bovine ovaries were used for recovery of cumulus-oocyte complexes (COCs) over eight replicates through May and June. On Day −1, COCs were collected and matured for 20h in oocyte maturation medium incubated at 38.5°C. On Day 0, matured oocytes were added to a solution of IVF-Tyrode's albumin lactate pyruvate (TALP) and either phosphate-buffered saline (PBS; control) or 100ngmL−1 NGF. Pooled frozen-thawed semen from two different bulls per replicate were added to the IVF solutions and incubated with COCs for 20h at 38.5°C in a humidified atmosphere of 5% CO2. On Day 1, zygotes were washed in HEPES-TALP, and cumulus cells were removed using 1% hyaluronidase. The zygotes were plated in synthetic oviductal fluid (SOF-BE2) culture medium and incubated at 38.5°C in a tri-gas chamber (5% CO2, 5% O2, and balanced N2). Cleavage rates were recorded at 24 and 48h, calculated by dividing the number of cleaved embryos by the total zygote count. Embryos were incubated until Day 8, when the rate of blastocysts was assessed. This study found that the treatment of IVF medium with NGF increased the cleavage rate of embryos after 48h (Control: 59%; NGF: 66%; P=0.04) and the hatched blastocyst percentage per oocyte on Day 8 (Control: 6.7%; NGF: 9.4%; P=0.01). The treatment did not affect the percentage of blastocysts per cleaved embryos (Control: 21%; NGF: 22%; P=0.16) or the hatched blastocyst rate at Day 8 (Control: 53%; NGF: 55%; P=0.67). These results show that NGF can act directly on the oocyte during fertilization to alter subsequent development, specifically through increased embryonic cleavage rates. Further studies are needed to assess different dosages of NGF in order to mitigate the detrimental effects of heat stress on oocyte competence for use in IVF. Follow-up studies using a whole-animal model are needed to understand the clinical relevance of these findings in the ability of embryos to promote maternal recognition of pregnancy.

PSX-A-23 Late-Breaking: Supplementation of Nerve Growth Factor (β-NGF) in the maturation medium and efficiency of in vitro embryo production in cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 365-365
Author(s):  
Lucas Gonçalves ◽  
Muller C Martins ◽  
Natalia Arle ◽  
Rafaela T Torres ◽  
Luisa Migilo ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Oocyte Maturation ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Nerve Growth ◽  
Maturation Medium ◽  
In Vitro Embryo Production ◽  
Blastocyst Rate

Abstract The aim of this study was to evaluate the supplementation of Nerve Growth Factor (β-NGF) in the maturation medium in in vitro embryo production routines. Antral follicles were aspirated from ovaries of cows obtained from slaughterhouses and then oocytes were selected for quality (grades I and II) for in vitro maturation and subjected to 4 successive in vitro embryo production routines (IVEP). Supplementation of 100 ng of β-NGF was performed in the oocyte maturation medium 22 hours before in vitro fertilization. 48 hours after fertilization of the oocytes, an analysis was made of their cleavage rate by counting blastomeres with the aid of a stereoscopic microscope (cleavage rate = number of embryos / number of initial oocytes). Seven days after fertilization, the blastocyst rate was determined through the relation to the number of oocytes that started cleavage and reached this stage of development (blastocyst rate = number of blastocyst / number of oocytes that started cleavage). To verify the existence of a difference between the supplemented and the non-supplemented groups, the paired T test was applied, using the Excel / Action software (Microsoft). In vitro embryo production routines supplemented with β-NGF in the maturation medium had, on average, a higher cleavage rate (P = 0.0072) and a higher blastocyst rate (P = 0.0033) compared to non-supplemented routines with β-NGF. In this study was demonstrated that Nerve Growth Factor supplementation in the maturation medium improves the efficiency of in vitro embryo production in cattle, and this protein has a probable action in the oocyte maturation process.

scholarly journals Nerve growth factor regulates axial rotation during early stages of chick embryo development

2012 ◽  
Vol 109 (6) ◽  
pp. 2009-2014 ◽  
Author(s):  
A. Manca ◽  
S. Capsoni ◽  
A. Di Luzio ◽  
D. Vignone ◽  
F. Malerba ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Chick Embryo ◽  
Embryo Development ◽  
Axial Rotation ◽  
Nerve Growth ◽  
Early Stages

Screening Natural Products for Effects on Nerve Growth Factor (NGF) Stimulated Neuritogenesis and Neuritic Outgrowth

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
ND Chaurasiya ◽  
R Sahu ◽  
V Samoylenko ◽  
M Ilias ◽  
LA Walker ◽  
...  
Keyword(s):  
Natural Products ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Neuritic Outgrowth

Nerve Growth Factor overexpression in Clara cells suppresses metastasis and tumor growth in a mouse model of experimental lung metastasis

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
K Seidler ◽  
A Sydykov ◽  
S Müller-Brüsselbach ◽  
R Müller ◽  
N Weißmann ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mouse Model ◽  
Tumor Growth ◽  
Lung Metastasis ◽  
Clara Cells ◽  
Nerve Growth

Degenerating and Painful Human Intervertebral Discs Release Pronociceptive Factors and Increase Neurite Sprouting and CGRP via Nerve Growth Factor

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376539-s-0034-1376539
Author(s):  
E. Krock ◽  
D. H. Rosenzweig ◽  
A. J. Chabot-Dore ◽  
P. Jarzem ◽  
M. H. Weber ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Intervertebral Discs ◽  
Nerve Growth
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