Effects of dietary roughage and sulfur in diets containing corn dried distillers grains with solubles on hydrogen sulfide production and fermentation by rumen microbes in vitro

2016 ◽  
Vol 94 (9) ◽  
pp. 3883-3893 ◽  
Author(s):  
E. Y. Binversie ◽  
M. Ruiz-Moreno ◽  
A. J. Carpenter ◽  
B. J. Heins ◽  
G. I. Crawford ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Distillers Grains ◽  
Rumen Microbes ◽  
Distillers Grains With Solubles ◽  
Dried Distillers Grains ◽  
Sulfide Production ◽  
Hydrogen Sulfide Production

scholarly journals Estimation of bacterial hydrogen sulfide production in vitro

2015 ◽  
Vol 7 (1) ◽  
pp. 28166 ◽  
Author(s):  
Amina Basic ◽  
Susanne Blomqvist ◽  
Anette Carlén ◽  
Gunnar Dahlén
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfide Production ◽  
Hydrogen Sulfide Production

Enhanced Sp1/YY1 Expression Directs CBS Transcription to Mediate VEGF-Stimulated Pregnancy-Dependent H 2 S Production in Human Uterine Artery Endothelial Cells

Hypertension ◽  
2021 ◽  
Author(s):  
Jin Bai ◽  
Dong-bao Chen
Keyword(s):  
Hydrogen Sulfide ◽  
Uterine Artery ◽  
Promoter Sequence ◽  
Vascular Endothelial ◽  
Promoter Activation ◽  
Sulfide Production ◽  
Hydrogen Sulfide Production ◽  
Specificity Protein ◽  
Endothelial Growth Factor

Pregnancy and VEGF (vascular endothelial growth factor) stimulate uterine artery endothelial cell (UAEC) hydrogen sulfide production via selectively upregulating CBS (cystathionine β-synthase) but not CSE (cystathionine γ-lyase) expression. This study was conducted to determine the mechanisms by which VEGF utilizes to stimulate pregnancy-dependent upregulation of CBS and hydrogen sulfide production in human UAEC. The proximal human CBS promoter contains 4 Sp1 (specificity protein 1; a/b/c/d) sites and 1 YY1 (Yin Yang 1) site; luciferase assays using reporter genes driven by human CBS promoter with a series of 5′-deletions identified a promoter sequence (−574 to −394) containing Sp1d and the YY1 sites critical for basal and VEGF-stimulated CBS promoter activation. VEGF stimulated pregnancy-dependent recruitment of Sp1 to Sp1d and YY1 to YY1 and also recruited YY1 to Sp1c and increased Sp1/YY1 association in pregnant human UAEC, suggesting formation of a Sp1/YY1 complex at the Sp1c site. Endothelial Sp1 and YY1 proteins were significantly greater in pregnant than nonpregnant human uterine artery. VEGF stimulated pregnancy-dependent Sp1 and YY1 protein expression in vitro. Treatment with Sp1 and YY1 siRNAs completely blocked Sp1/YY1-mediated pregnancy-dependent CBS protein upregulation and hydrogen sulfide production by VEGF in human UAEC. VEGF did not trans -activate CSE promoter or increase CSE expression, and Sp1/YY1 knockdown did not affect CSE expression in human UAEC. Thus, pregnancy augments EC Sp1 and YY1 expression and promotes the recruitment of Sp1/YY1 to their DNA-binding sequences in proximal human CBS promoter to upregulate CBS transcription, underlying a novel mechanism to mediate VEGF-stimulated pregnancy-dependent endothelial hydrogen sulfide production in the human uterine artery.

In vitro gas production including methane from bermudagrasses supplemented with dried distillers grains with solubles

2020 ◽  
Vol 36 (2) ◽  
pp. 172-182
Author(s):  
W.B. Smith ◽  
M.D. Miller ◽  
W.L. Crossland ◽  
T.R. Callaway ◽  
L.O. Tedeschi ◽  
...  
Keyword(s):  
Gas Production ◽  
Distillers Grains ◽  
Distillers Grains With Solubles ◽  
Dried Distillers Grains ◽  
In Vitro Gas Production
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