Supplementation of corn dried distillers' grains plus solubles to gestating beef cows fed low-quality forage: II. Impacts on uterine blood flow, circulating estradiol-17β and progesterone, and hepatic steroid metabolizing enzyme activity1

2016 ◽  
Vol 94 (11) ◽  
pp. 4619-4628 ◽  
Author(s):  
V. C. Kennedy ◽  
B. R. Mordhorst ◽  
J. J. Gaspers ◽  
M. L. Bauer ◽  
K. C. Swanson ◽  
...  
Keyword(s):  
Blood Flow ◽  
Beef Cows ◽  
Distillers Grains ◽  
Uterine Blood Flow ◽  
Dried Distillers Grains ◽  
Metabolizing Enzyme ◽  
Estradiol 17Β

Regulation of types I and III NOS in ovine uterine arteries by daily and acute estrogen exposure

2000 ◽  
Vol 278 (6) ◽  
pp. H2134-H2142 ◽  
Author(s):  
Walid A. Salhab ◽  
Philip W. Shaul ◽  
Blair E. Cox ◽  
Charles R. Rosenfeld
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Smooth Muscle ◽  
Nitric Oxide Synthases ◽  
Uterine Blood Flow ◽  
Calcium Dependent ◽  
Uterine Arteries ◽  
Estrogen Exposure ◽  
The Media ◽  
Estradiol 17Β

Nitric oxide contributes to estrogen-mediated uterine vasodilation; however, the nitric oxide synthases (NOS) involved and their location within uterine arteries are incompletely documented. We investigated the effects of repetitive daily and acute estradiol-17β (E2β) exposure on uterine hemodynamics and NOS abundance and localization in uterine arteries from nonpregnant ovariectomized ewes receiving daily intravenous E2β (1 μg/kg, n = 5) or no E2β ( n = 7) for 5 days to determine NOS abundance, cGMP contents, and NOS immunohistochemistry. Daily E2β increased basal and E2β-mediated rises in uterine blood flow (UBF) 36 and 43% (<0.01), respectively, calcium-dependent NOS activity 150% ( P < 0.02) in endothelium-intact and -denuded (∼40% of total NOS) arteries, and cGMP contents 39% ( P < 0.05). Endothelial (eNOS) was detected in luminal endothelium, whereas neuronal NOS (nNOS) protein was only in the media. A second group of ewes received E2β (1 μg/kg iv) for 4 days and acute intravenous E2β ( n = 8) or vehicle ( n= 4) on day 5. UBF rose 5.5-fold ( P < 0.001) 115 min after E2β, at which time only endothelium-derived calcium-dependent NOS activity increased 30 ± 13% ( P < 0.05). Daily E2β enhances basal and E2β-mediated increases in UBF, which parallel increases in endothelium-derived eNOS and smooth muscle-derived nNOS. Acute E2β, however, selectively increases endothelium-derived eNOS.

scholarly journals Large Conductance Ca2+-Activated and Voltage-Activated K+ Channels Contribute to the Rise and Maintenance of Estrogen-Induced Uterine Vasodilation and Maintenance of Blood Pressure

Endocrinology ◽  
2012 ◽  
Vol 153 (12) ◽  
pp. 6012-6020 ◽  
Author(s):  
Charles R. Rosenfeld ◽  
Timothy Roy
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Blood Flow ◽  
Uterine Artery ◽  
K Channel ◽  
Uterine Blood Flow ◽  
K Channels ◽  
Channel Inhibition ◽  
Estradiol 17Β ◽  
Cgmp Synthesis

Abstract Uterine blood flow (UBF) increases greater than 4-fold 90 min after systemic estradiol-17β (E2β) in nonpregnant sheep and remains elevated longer than 6–8 h; mean arterial pressure (MAP) is unchanged. Large-conductance Ca+2-activated (BKCa) and voltage-activated (KV) K+ channels contribute to the acute rise in UBF; their role in maintaining UBF and MAP longer than 90 min is unknown. We examined this in five nonpregnant, ovariectomized ewes with uterine artery (UA) flow probes and catheters in a UA for infusion of K+ channel inhibitors and uterine vein to sample venous effluent. Animals received systemic E2β (1.0 μg/kg; control), E2β+UA tetraethylammonium (TEA; 0.4–0.8 mm, n = 4), and E2β+UA 4-aminopyridine (4-AP; 0.01–0.08 mm, n = 4) to block BKCa and KV, respectively, while monitoring MAP, heart rate, and UBF. Uterine cGMP synthesis was measured. Ninety minutes after E2β, UBF rose 4.5-fold, uterine vascular resistance (UVR) fell greater than 5-fold and MAP was unchanged [78 ± 0.8 (sem) vs. 77 ± 1.5 mm Hg] in control studies and before UA inhibition with TEA and 4-AP. Between 90 and 120min, UBF, UVR, and MAP were unchanged after E2β alone. E2β+TEA dose dependently decreased ipsilateral UBF and increased UVR (24 ± 8.9 and 38 ± 16%, respectively, at 0.8 mm; P &lt; 0.03); MAP was unchanged. Contralateral UBF/UVR were unaffected. E2β+4-AP also dose dependently decreased ipsilateral UBF and increased UVR (27 ± 5.3 and 76 ± 18%, respectively, at 0.08 mm; P &lt; 0.001); however, MAP rose 27 ± 6.9% (P ≤ 0.006). E2β increased uterine cGMP synthesis greater than 3.5-fold and was unaffected by local K+ channel inhibition. BKCa and KV contribute to the rise and maintenance of E2β-induced uterine vasodilation, which is partially cGMP dependent. Systemic vascular KV also contributes to maintaining MAP after systemic E2β.

scholarly journals Effects of maternal nutrient restriction followed by realimentation during midgestation on uterine blood flow in beef cows

2014 ◽  
Vol 81 (9) ◽  
pp. 1248-1256.e3 ◽  
Author(s):  
L.E. Camacho ◽  
C.O. Lemley ◽  
L.D. Prezotto ◽  
M.L. Bauer ◽  
H.C. Freetly ◽  
...  
Keyword(s):  
Blood Flow ◽  
Beef Cows ◽  
Nutrient Restriction ◽  
Uterine Blood Flow

Supplementation of dried distillers grains with solubles to beef cows consuming low-quality forage during late gestation and early lactation1

2012 ◽  
Vol 90 (6) ◽  
pp. 2014-2025 ◽  
Author(s):  
S. J. Winterholler ◽  
C. P. McMurphy ◽  
G. L. Mourer ◽  
C. R. Krehbiel ◽  
G. W. Horn ◽  
...  
Keyword(s):  
Beef Cows ◽  
Late Gestation ◽  
Distillers Grains ◽  
Distillers Grains With Solubles ◽  
Dried Distillers Grains
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