scholarly journals High-affinity glutamate transporter and glutamine synthetase content in longissimus dorsi and adipose tissues of growing Angus steers differs among suckling, weanling, backgrounding, and finishing production stages1

2016 ◽  
Vol 94 (3) ◽  
pp. 1267-1275 ◽  
Author(s):  
J. C. Matthews ◽  
J. Huang ◽  
G. Rentfrow
Keyword(s):  
Glutamine Synthetase ◽  
Glutamate Transporter ◽  
Longissimus Dorsi ◽  
Adipose Tissues ◽  
High Affinity

Conformationally restricted inhibitors of the high affinity -glutamate transporter

1993 ◽  
Vol 3 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Richard J. Bridges ◽  
Frank E. Lovering ◽  
John M. Humphrey ◽  
Mark S. Stanley ◽  
Tracy N. Blakely ◽  
...  
Keyword(s):  
Glutamate Transporter ◽  
Conformationally Restricted ◽  
High Affinity

scholarly journals Cellular distribution of a high-affinity glutamate transporter in the nervous system of the cabbage looperTrichoplusia ni

2002 ◽  
Vol 205 (17) ◽  
pp. 2605-2613 ◽  
Author(s):  
Richard B. Gardiner ◽  
Kyrre Ullensvang ◽  
Niels C. Danbolt ◽  
Stanley Caveney ◽  
B. Cameron Donly
Keyword(s):  
Nervous System ◽  
Glial Cells ◽  
Trichoplusia Ni ◽  
Neuromuscular Junctions ◽  
Glutamate Transporter ◽  
Extracellular Fluid ◽  
Sensory Nerves ◽  
Cabbage Looper ◽  
Microscopical Examination ◽  
High Affinity

SUMMARYGlutamate functions as a neurotransmitter in the central nervous system(CNS) and neuromuscular junctions in insects. High-affinity glutamate transporters are responsible for keeping the resting levels of excitatory amino acids below the synaptic activation threshold by removing them from the extracellular fluid, thereby preventing them from reaching toxic levels. Peptides representing the N- and C-terminal regions of a glutamate transporter cloned from the cabbage looper caterpillar (Trichoplusia ni) were synthesized and used to generate polyclonal antibodies. The antibodies produced immunohistochemical staining in both muscular and nervous system T. ni tissues. Neuromuscular junctions in the skeletal muscles produced the most intense labelling, but no visceral muscle or sensory nerves were labelled. In the CNS, the neuropile of the ganglia, but not the connectives, gave a diffuse staining. Electron microscopical examination of ganglia and neuromuscular junctions showed that the plasma membrane of glial cells, but not that of neurons was labelled, in agreement with the notion that most of the glutamate uptake sites in this insect are in glial cells.

The alterations of glutamate transporter 1 and glutamine synthetase in the rat brain of a learned helplessness model of depression

2020 ◽  
Vol 237 (8) ◽  
pp. 2547-2553
Author(s):  
Kouhei Yoshino ◽  
Yasunori Oda ◽  
Makoto Kimura ◽  
Hiroshi Kimura ◽  
Masahito Nangaku ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Rat Brain ◽  
Learned Helplessness ◽  
Glutamate Transporter ◽  
Glutamate Transporter 1 ◽  
Helplessness Model

Localization of the high-affinity glutamate transporter EAAC1 in rat kidney

1997 ◽  
Vol 273 (6) ◽  
pp. F1023-F1029 ◽  
Author(s):  
Chairat Shayakul ◽  
Yoshikatsu Kanai ◽  
Wen-Sen Lee ◽  
Dennis Brown ◽  
Jeffrey D. Rothstein ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rat Kidney ◽  
Cell Volume Regulation ◽  
Glutamate Transporter ◽  
Transport Systems ◽  
Kidney Cortex ◽  
Acid Base Balance ◽  
Western Blots ◽  
High Affinity

Most amino acids filtered by the glomerulus are reabsorbed in the kidney via specialized transport systems. Recently, the cDNA encoding a high-affinity glutamate transporter, EAAC1, has been isolated and shown to be expressed at high levels in the kidney. To determine the potential role of EAAC1 in renal acidic amino acid reabsorption, the distribution of EAAC1 mRNA and protein in rat kidney was examined. In situ hybridization revealed that EAAC1 mRNA is expressed predominantly in S2 and S3 segments of the proximal tubules and at low levels in the inner stripe of outer medulla and inner medulla. Polyclonal antibodies raised against the carboxy terminus of EAAC1 recognized a single band of ∼70 kDa on Western blots of membrane protein from kidney cortex and medulla. Immunofluorescence microscopy revealed intense signals in the luminal membrane of S2 and S3 segments and weaker signals in S1 segments, descending thin limbs of long-loop nephrons, medullary thick ascending limbs, and distal convoluted tubules. These results are consistent with EAAC1 encoding the previously described apical high-affinity glutamate transporter in the kidney that mediates reabsorption of acidic amino acids in tubules beyond early proximal tubule S1 segments. Potential additional roles of EAAC1 in acid/base balance, cell volume regulation, and amino acid metabolism are discussed.

Discrete cellular and subcellular localization of glutamine synthetase and the glutamate transporter GLAST in the rat vestibular end organ

Neuroscience ◽  
1997 ◽  
Vol 79 (4) ◽  
pp. 1137-1144 ◽  
Author(s):  
Y Takumi ◽  
A Matsubara ◽  
N.C Danbolt ◽  
J.H Laake ◽  
J Storm-Mathisen ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Subcellular Localization ◽  
Glutamate Transporter

scholarly journals Effect of Benzodiazepines on the Epithelial and Neuronal High-Affinity Glutamate Transporter EAAC1

2002 ◽  
Vol 73 (6) ◽  
pp. 2389-2396 ◽  
Author(s):  
Mònica Palmada ◽  
Christoph Böhmer ◽  
Josep Joan Centelles ◽  
Rolf K. H. Kinne
Keyword(s):  
Glutamate Transporter ◽  
High Affinity
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