Supplementation with spermine during in vitro maturation of porcine oocytes improves early embryonic development after parthenogenetic activation and somatic cell nuclear transfer1

2016 ◽  
Vol 94 (3) ◽  
pp. 963-970 ◽  
Author(s):  
J. X. Jin ◽  
S. Lee ◽  
C. Khoirinaya ◽  
A. Oh ◽  
G. A. Kim ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Somatic Cell ◽  
In Vitro Maturation ◽  
Early Embryonic Development ◽  
Parthenogenetic Activation

scholarly journals Effect of D-Glucuronic Acid and N-acetyl-D-Glucosamine Treatment during In Vitro Maturation on Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1034
Author(s):  
Joohyeong Lee ◽  
Eunhye Kim ◽  
Seon-Ung Hwang ◽  
Lian Cai ◽  
Mirae Kim ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
In Vitro Maturation ◽  
Glucuronic Acid ◽  
Cumulus Cell ◽  
Cortical Granule ◽  
Oocyte Diameter

This study aimed to examine the effects of treatment with glucuronic acid (GA) and N-acetyl-D-glucosamine (AG), which are components of hyaluronic acid (HA), during porcine oocyte in vitro maturation (IVM). We measured the diameter of the oocyte, the thickness of the perivitelline space (PVS), the reactive oxygen species (ROS) level, and the expression of cumulus cell expansion and ROS-related genes and examined the cortical granule (CG) reaction of oocytes. The addition of 0.05 mM GA and 0.05 mM AG during the first 22 h of oocyte IVM significantly increased oocyte diameter and PVS size compared with the control (non-treatment). The addition of GA and AG reduced the intra-oocyte ROS content and improved the CG of the oocyte. GA and AG treatment increased the expression of CD44 and CX43 in cumulus cells and PRDX1 and TXN2 in oocytes. In both the chemically defined and the complex medium (Medium-199 + porcine follicular fluid), oocytes derived from the GA and AG treatments presented significantly higher blastocyst rates than the control after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). In conclusion, the addition of GA and AG during IVM in pig oocytes has beneficial effects on oocyte IVM and early embryonic development after PA and SCNT.

Effect of Antioxidants Treated During In Vitro Maturation of Porcine Oocytes on the Early Embryonic Development.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 162-163
Author(s):  
Changyong Choe ◽  
Sang-Rae Cho ◽  
Hyun-Jong Kim ◽  
Sun-Ho Choi ◽  
Sung-Hyum Yeon ◽  
...  
Keyword(s):  
Embryonic Development ◽  
In Vitro Maturation ◽  
Early Embryonic Development

Kisspeptin Enhances Porcine Oocyte In Vitro Maturation but May Adversely Affect Early Embryonic Development.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 434-434
Author(s):  
Islam M. Saadeldin ◽  
Ok Jae Koo ◽  
Jung Taek Kang ◽  
Dae Kee Kwon ◽  
Solji Park ◽  
...  
Keyword(s):  
Embryonic Development ◽  
In Vitro Maturation ◽  
Early Embryonic Development ◽  
Porcine Oocyte
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