Substrate elasticity affects bovine satellite cell activation kinetics in vitro

2013 ◽  
Vol 91 (5) ◽  
pp. 2083-2090 ◽  
Author(s):  
M. R. Lapin ◽  
J. M. Gonzalez ◽  
S. E. Johnson
Keyword(s):  
Satellite Cell ◽  
Cell Activation ◽  
Activation Kinetics ◽  
Satellite Cell Activation ◽  
Substrate Elasticity

scholarly journals Culture conditions influence satellite cell activation and survival of single myofibers

2018 ◽  
Vol 28 (2) ◽  
Author(s):  
Alessandra Renzini ◽  
Anna Benedetti ◽  
Marina Bouché ◽  
Leopoldo Silvestroni ◽  
Sergio Adamo ◽  
...  
Keyword(s):  
Satellite Cell ◽  
Satellite Cells ◽  
Cell Activation ◽  
Culture Conditions ◽  
Experimental Conditions ◽  
Direct Observations ◽  
Satellite Cell Activation ◽  
Indispensable Tool

Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.

scholarly journals An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

2021 ◽  
Vol 2 (2) ◽  
pp. 100482
Author(s):  
Rodrigo Canibano-Fraile ◽  
Emma Boertjes ◽  
Stela Bozhilova ◽  
W.W.M. Pim Pijnappel ◽  
Gerben J. Schaaf
Keyword(s):  
Satellite Cell ◽  
Cell Activation ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Satellite Cell Activation

Hepatocyte growth factor affects satellite cell activation and differentiation in regenerating skeletal muscle

2000 ◽  
Vol 278 (1) ◽  
pp. C174-C181 ◽  
Author(s):  
Kristy J. Miller ◽  
Deepa Thaloor ◽  
Sarah Matteson ◽  
Grace K. Pavlath
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Satellite Cell ◽  
Cell Activation ◽  
Inhibitory Effect ◽  
Satellite Cell Activation ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF) is the only known growth factor that activates quiescent satellite cells in skeletal muscle. We hypothesized that local delivery of HGF may enhance regeneration after trauma by increasing the number of myoblasts available for restoring normal tissue architecture. Injection of HGF into muscle at the time of injury increases myoblast number but does not enhance tissue repair as determined using quantitative histological analyses. Rather, depending on the dose and the timing of HGF administration relative to the injury, regeneration can be inhibited. The greatest inhibitory effect is observed when HGF is administered on the day of injury and continued for 3 days, corresponding to the time when satellite cell activation, proliferation, and early differentiation normally occur. To establish a mechanism for this inhibition, we show that HGF can act directly on primary muscle cells to block differentiation. These results demonstrate that 1) exogenous HGF synergizes with factors in damaged muscle to increase myoblast number, 2) regeneration is not regulated solely by myoblast number, and 3) HGF inhibits muscle differentiation both in vitro and in vivo.

scholarly journals Lack of robust satellite cell activation and muscle regeneration during the progression of Pompe disease

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Gerben J. Schaaf ◽  
Tom JM van Gestel ◽  
Esther Brusse ◽  
Robert M. Verdijk ◽  
Irenaeus FM de Coo ◽  
...  
Keyword(s):  
Satellite Cell ◽  
Pompe Disease ◽  
Muscle Regeneration ◽  
Cell Activation ◽  
Satellite Cell Activation

scholarly journals Metformin Delays Satellite Cell Activation and Maintains Quiescence

2019 ◽  
Vol 2019 ◽  
pp. 1-19 ◽  
Author(s):  
Theodora Pavlidou ◽  
Milica Marinkovic ◽  
Marco Rosina ◽  
Claudia Fuoco ◽  
Simone Vumbaca ◽  
...  
Keyword(s):  
Satellite Cell ◽  
Muscle Tissue ◽  
Satellite Cells ◽  
Cell Activation ◽  
Myogenic Differentiation ◽  
Metabolic State ◽  
Cell Pool ◽  
Age Related ◽  
Satellite Cell Activation

The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of muscle tissue. These disorders are associated with the depletion of the satellite cell pool or with the loss of satellite cell functionality. Recently, the establishment and maintenance of quiescence in satellite cells have been linked to their metabolic state. In this work, we aimed to modulate metabolism in order to preserve the satellite cell pool. We made use of metformin, a calorie restriction mimicking drug, to ask whether metformin has an effect on quiescence, proliferation, and differentiation of satellite cells. We report that satellite cells, when treated with metformin in vitro, ex vivo, or in vivo, delay activation, Pax7 downregulation, and terminal myogenic differentiation. We correlate the metformin-induced delay in satellite cell activation with the inhibition of the ribosome protein RPS6, one of the downstream effectors of the mTOR pathway. Moreover, in vivo administration of metformin induces a belated regeneration of cardiotoxin- (CTX-) damaged skeletal muscle. Interestingly, satellite cells treated with metformin immediately after isolation are smaller in size and exhibit reduced pyronin Y levels, which suggests that metformin-treated satellite cells are transcriptionally less active. Thus, our study suggests that metformin delays satellite cell activation and differentiation by favoring a quiescent, low metabolic state.

scholarly journals Castration induces satellite cell activation that contributes to skeletal muscle maintenance

2018 ◽  
Vol 1 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Alanna Klose ◽  
Wenxuan Liu ◽  
Nicole D. Paris ◽  
Sophie Forman ◽  
John J. Krolewski ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cell ◽  
Cell Activation ◽  
Satellite Cell Activation

Satellite cell activation and retention of muscle regenerative potential after long-term denervation

Stem Cells ◽  
2020 ◽  
Vol 39 (3) ◽  
pp. 331-344
Author(s):  
Alvin Wong ◽  
Steven M. Garcia ◽  
Stanley Tamaki ◽  
Katharine Striedinger ◽  
Emilie Barruet ◽  
...  
Keyword(s):  
Satellite Cell ◽  
Cell Activation ◽  
Satellite Cell Activation

Satellite cell activation after muscle damage in young and adult rats

1995 ◽  
Vol 242 (3) ◽  
pp. 329-336 ◽  
Author(s):  
S. C. J. M. Jacobs ◽  
J. H. J. Wokke ◽  
P. R. Bär ◽  
A. L. Bootsma
Keyword(s):  
Muscle Damage ◽  
Satellite Cell ◽  
Cell Activation ◽  
Adult Rats ◽  
Satellite Cell Activation
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