Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle1

2011 ◽  
Vol 89 (1) ◽  
pp. 12-22 ◽  
Author(s):  
T. Matsuhashi ◽  
S. Maruyama ◽  
Y. Uemoto ◽  
N. Kobayashi ◽  
H. Mannen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gene Polymorphisms ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Growth Hormone Gene ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

scholarly journals Sterol regulatory element binding transcription factor 1 (SREBF1) polymorphism and milk fatty acid composition

2013 ◽  
Vol 96 (4) ◽  
pp. 2605-2616 ◽  
Author(s):  
R.A. Nafikov ◽  
J.P. Schoonmaker ◽  
K.T. Korn ◽  
K. Noack ◽  
D.J. Garrick ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Regulatory Element ◽  
Milk Fatty Acid ◽  
Sterol Regulatory Element ◽  
Transcription Factor 1

Regulation of fatty acid synthase expression in breast cancer by sterol regulatory element binding protein-1c

2003 ◽  
Vol 282 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Y.u-A.n Yang ◽  
Patrice J. Morin ◽  
Wan Fang Han ◽  
Tinghua Chen ◽  
Daniel M. Bornman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Binding Protein ◽  
Regulatory Element ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

scholarly journals Insulin induction of glucokinase and fatty acid synthase in hepatocytes: analysis of the roles of sterol-regulatory-element-binding protein-1c and liver X receptor

2006 ◽  
Vol 399 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Franck Hansmannel ◽  
Sylvie Mordier ◽  
Patrick B. Iynedjian
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Glycogen Synthase ◽  
Binding Protein ◽  
Regulatory Element ◽  
Positive Control ◽  
Liver X Receptor ◽  
Transcription Activator ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

The transcription activator SREBP-1c (sterol-regulatory-element-binding protein-1c) is induced by insulin in the liver and is considered a master regulator of lipogenic genes such as FASN (fatty acid synthase). The question of whether SREBP-1c is also a mediator of insulin action on the regulatory enzyme of glucose metabolism GCK (glucokinase) is controversial. In the present paper, we induced SREBP-1c to various levels with insulin or the liver X receptor ligand T0901317 in primary hepatocytes and asked if these levels correlated with those of GCK or FASN mRNA expression, using the latter as positive control. Insulin and T0901317 triggered the accumulation of precursor and processed forms of SREBP-1c to similar levels and with comparable kinetics, and both effectors together caused synergistic increases in SREBP-1c protein levels. These effects were accompanied by commensurate elevation of FASN mRNA, notably by a synergistic response to both effectors. By contrast, GCK mRNA was unresponsive to T0901317 and was induced only by insulin. Treatment of hepatocytes with insulin and/or T0901317 resulted in the recruitment of SREBP-1c to the FASN promoter as shown by chromatin immunoprecipitation, whereas SREBP-1c did not bind to the GCK promoter. Lastly, we observed that the glycogen synthase kinase-3 inhibitor SB216763 produced a small increase in SREBP-1c protein level, which was further augmented in the presence of T0901317. The level of FASN mRNA varied in parallel with SREBP-1c, while GCK mRNA was unaffected. Collectively, these results showed that increases in SREBP-1c were neither necessary nor sufficient for GCK induction in hepatocytes, while at the same time they underscored the role of SREBP-1c as a key regulator of FASN.

scholarly journals Fatty acid composition and regulatory gene expression in late-term embryos of ACRB and COBB broilers

2019 ◽  
Author(s):  
Shengchen Su ◽  
Yidi Wang ◽  
Miyoung Suh ◽  
Michael Azain ◽  
Woo Kyun Kim
Keyword(s):  
Gene Expression ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Embryonic Development ◽  
Acid Composition ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Regulatory Gene ◽  
Production Performance ◽  
Apo B

Abstract Background:Cobb broilers (COBB) have been heavily selected for their production performance in the past several decades, while the Athens Canadian Random Bred (ACRB) chickens, a meat-type breed, has been kept as a non-selected control strain. The purpose of this study was to compare these two lines of chickens at late-embryonic development and identify the molecular markers and fatty acid profiles underlining their differences in growth performance due to selection. Results: COBB had higher egg weight, embryo weight, and breast and fat ratio. The gene expression in the liver showed an interaction between age and breed on FASN (fatty acid synthase) expression with the highest level in COBB at E18. ACRB had higher Apo-B (apolipoprotein B) and MTTP (microsomal triglyceride transfer protein) expression, but lower SREBP-1(regulatory element-binding protein 1) expression compared to COBB. No difference was found in myogenesis gene expression in the muscle between two breeds. For the fatty acid composition, muscle was largely affected by both breed and age. Yolk and liver were affected mainly by breed and age, respectively. Constant interaction effects in docosahexaenoic acid (DHA), indicating the highest level in all the tested tissues of ACRB at E14 and the constant main effects with higher myristic, palmitic and gondoic, but lower linolenic acid in the liver and yolk of COBB compared to in those of ACRB. At last, fat accumulation in the liver had no obvious difference between the breeds but was higher when embryo was older. Conclusions: Broiler breed affects egg, embryo and tissue weight, as well as FA composition in initial egg yolk and throughout the embryonic development. The highest docosahexaenoic percentage was observed in ACRB, indicating that genetic selection may result in fatty acid profile changes in chicken tissues and eggs.

scholarly journals Functional antagonism between inhibitor of DNA binding (Id) and adipocyte determination and differentiation factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP-1c) trans-factors for the regulation of fatty acid synthase promoter in adipocytes

1999 ◽  
Vol 344 (3) ◽  
pp. 873-880 ◽  
Author(s):  
Marthe MOLDES ◽  
Muriel BOIZARD ◽  
Xavier LE LIEPVRE ◽  
Bruno FÈVE ◽  
Isabelle DUGAIL ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dna Binding ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Expression Vectors ◽  
Element Binding Protein ◽  
Sterol Regulatory Element ◽  
Inhibitor Of Dna Binding ◽  
Isolated Rat

We show that Id (inhibitor of DNA binding) 2 and Id3, dominant negative members of the helix-loop-helix (HLH) family, interact with the adipocyte determination and differentiation factor 1 (ADD1)/sterol regulatory element-binding protein (SREBP) 1c, a transcription factor of the basic HLH-leucine zipper family that controls the expression of several key genes of adipose metabolism. Gel mobility-shift assays performed with in vitro-translated ADD1, Id2 or Id3 proteins and a fatty acid synthase (FAS) promoter oligonucleotide showed evidence for a marked inhibition of the formation of DNA-ADD1 complexes by Id2 or Id3 proteins. Co-immunoprecipitation studies using in vitro-translated proteins demonstrated further the physical interaction of Id and ADD1/SREBP-1c proteins in the absence of DNA. Using the FAS gene as a model of an ADD1-regulated promoter in transiently transfected isolated rat adipocytes or mature 3T3-L1 adipocytes, a potent inhibition of the activity of the FAS-chloramphenicol acetyltransferase reporter gene was observed by overexpression of Id2 or Id3. Reciprocally, co-transfection of Id3 antisense and ADD1 expression vectors in preadipocytes potentiated the ADD1/SREBP-1c effect on the FAS promoter activity. Finally, in the non adipogenic NIH-3T3 cell line, most of the ADD1-mediated trans-activation of the FAS promoter was counteracted by co-transfection of Id2 or Id3 expression vectors. Previous studies have indicated Id gene expression to be down-regulated during adipogenesis [Moldes, Lasnier, Fève, Pairault and Djian (1997) Mol. Cell. Biol. 17, 1796-1804]. We here demonstrated that there was a dramatic rise of Id2 and Id3 mRNA levels when 3T3-L1 adipocytes or isolated rat fat cells were exposed to lipolytic and anti-lipogenic agents, forskolin and isoproterenol. Taken together, our data show that Id products are functionally involved in modulating ADD1/SREBP-1c transcriptional activity, and thus lipogenesis in adipocytes.

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