Nuclear receptor and nuclear receptor target gene messenger ribonucleic acid levels at different sites of the gastrointestinal tract and in liver of healthy dogs

2006 ◽  
Vol 84 (10) ◽  
pp. 2684-2691 ◽  
Author(s):  
F. N. C. Gropp ◽  
D. L. Greger ◽  
C. Morel ◽  
S. Sauter ◽  
J. W. Blum
Keyword(s):  
Gastrointestinal Tract ◽  
Nuclear Receptor ◽  
Ribonucleic Acid ◽  
Target Gene ◽  
Messenger Ribonucleic Acid ◽  
Healthy Dogs

scholarly journals 17β-Hydroxysteroid Dehydrogenase Type 1 and 2 Expression in the Human Fetus1

2000 ◽  
Vol 85 (1) ◽  
pp. 410-416 ◽  
Author(s):  
Junji Takeyama ◽  
Takashi Suzuki ◽  
Gen Hirasawa ◽  
Yasunari Muramatsu ◽  
Hiroshi Nagura ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Epithelial Cells ◽  
Ribonucleic Acid ◽  
Estrogenic Activity ◽  
Expression Patterns ◽  
Hydroxysteroid Dehydrogenase ◽  
Pcr Analysis ◽  
Maternal Circulation ◽  
Messenger Ribonucleic Acid

The present study investigates the expression patterns of 17β-hydroxysteroid dehydrogenase (17βHSD) isozymes in human fetal tissues to understand how estrogenic activity is regulated in the human fetus. Using enzyme assay, high 17βHSD activity was detected in the placenta and liver, and low levels of 17βHSD activity were also present in the gastrointestinal tract and kidney. After Northern blot analysis, we detected the messenger ribonucleic acid for 17βHSD type 1 (17βHSD1) only in the placenta, whereas that for 17βHSD type 2 (17βHSD2) was detected in the placenta, liver, gastrointestinal tract, and urinary tract at 20 gestational weeks. In RT-PCR analysis of the messenger ribonucleic acid transcripts, 17βHSD1 was predominantly expressed in the placenta, brain, heart, lung, and adrenal, whereas 17βHSD2 expression was predominantly detected in the liver, gastrointestinal tract, and kidney. In addition, we detected 17βHSD2 immunoreactive protein in surface epithelial cells of the stomach, absorptive epithelial cells of the small intestine and colon, hepatocytes of the liver, and interstitial cells surrounding the urinary tubules of the renal medulla. 17βHSD2 in these tissues may be functioning in the prevention of in utero exposure of the fetus to excessive estradiol from the maternal circulation and amniotic fluids.

Expression of Calbindin-D9k Messenger Ribonucleic Acid in the Gastrointestinal Tract of Dairy Cattle

2002 ◽  
Vol 49 (9) ◽  
pp. 461-465 ◽  
Author(s):  
N. Yamagishi ◽  
Y. A. Yukawa ◽  
N. Ishiguro ◽  
S. Soeta ◽  
I. H. Lee ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Dairy Cattle ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
Calbindin D9k

scholarly journals Quantitative Measurement of Ovalbumin Messenger Ribonucleic Acid Activity

1973 ◽  
Vol 248 (6) ◽  
pp. 2031-2039 ◽  
Author(s):  
Robert E. Rhoads ◽  
G. Stanley McKnight ◽  
Robert T. Schimke
Keyword(s):  
Ribonucleic Acid ◽  
Quantitative Measurement ◽  
Messenger Ribonucleic Acid
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