1607 Effect of nitrate, monensin, and the combination of additives on rumen fermentation using a semicontinuous culture system

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 781-782
Author(s):  
M. Capelari ◽  
K. A. Johnson ◽  
B. Latack ◽  
J. Roth ◽  
W. Powers
Keyword(s):  
Culture System ◽  
Rumen Fermentation ◽  
Semicontinuous Culture

scholarly journals Design and development of a continuous culture system for studying rumen fermentation

1989 ◽  
Vol 61 (5) ◽  
pp. 463-473
Author(s):  
Harri Miettinen ◽  
Jouko Setälä
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Artificial Saliva ◽  
Present Report ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Liquid Volume ◽  
Desktop Computer ◽  
Continuous Culture System

The present report describes an in vitro continuous culture system to simulate rumen fermentation. The complete assembly consists of six culture vessels (liquid volume 700 ml) fed twice daily with finely ground feed. The artificial saliva enters the vessel continuously, and the effluent leaves it continuously through the overflow port. The intermittent stirring of the fermentor content and the pH regulation are automatically controlled by a desktop computer. Two replicate experiments with ten fermentors given a diet of silage (50 %) and barley (50 %) were made in order to evaluate the system. The results indicate that the system reaches steady-state conditions within three to five days, ammonia concentration being an exeption. It takes for the ammonia concentration approximately 11—14 days to stabilize. The plateau values for the total volatile fatty acid (VFA) concentrations, the molar proportions of individual VFAs, and the ammonia concentrations were found to be within the accepted range in the rumen of animals given similar diets or in other artificial rumen systems. There was a tenfold decrease in the numbers of protozoa in the fermentors during the first four days of incubation. However, the average plateau value for the protozoa numbers (2.5 x 104/ml) is in the same range as in the dual flow continuous culture systems. The efficiency of the microbial N production was higher than that usually observed in vivo or in vitro (45 vs. 30 g/kg organic matter digested). The results indicate that this continuous culture system provides a reasonable estimate of rumen fermentation.

094 The effect of two additives on ruminal fermentation using a semicontinuous culture system

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 47-47
Author(s):  
M. Capelari ◽  
K. A. Johnson ◽  
B. Latack ◽  
J. Roth ◽  
W. Powers
Keyword(s):  
Culture System ◽  
Ruminal Fermentation ◽  
Semicontinuous Culture

scholarly journals Effects of hydrolyzed and live yeasts on rumen microbial fermentation in a semicontinuous culture system (Rusitec)

2015 ◽  
Vol 39 ◽  
pp. 556-559 ◽  
Author(s):  
Hakan ÖZTÜRK ◽  
Yasemin SALGIRLI DEMİRBAŞ ◽  
Farah Gönül AYDIN ◽  
İlksin PİŞKİN ◽  
Ferhunde Melis ÜNLER ◽  
...  
Keyword(s):  
Culture System ◽  
Microbial Fermentation ◽  
Semicontinuous Culture
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