1144 Wingless-type mouse mammary tumor virus integration site (WNT) regulation of ovarian thec cells of cattle

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 549-549 ◽  
Author(s):  
L. J. Spicer
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Integration Site ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Virus Integration ◽  
Virus Integration Site

Wingless-type mouse mammary tumor virus integration site (WNT) regulation of bovine theca cells

2021 ◽  
Author(s):  
Leon J Spicer
Keyword(s):  
Growth Factor ◽  
Mouse Mammary Tumor Virus ◽  
Integration Site ◽  
Mammary Tumor Virus ◽  
Theca Cells ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Virus Integration ◽  
Virus Integration Site

Abstract Ovarian paracrine mediation by components of the wingless-type mouse mammary tumor virus integration site ligands (WNT1 to 11) and their receptors, Frizzled family members (FZD1 to 10), has been proposed. Secreted truncated forms of FZD proteins (e.g., SFRP4) block the action of WNT ligands. Dickkopf-1 (DKK1) is another WNT antagonist, and R-spondin-1 (RSPO1) is one of a group of four secreted proteins that enhance WNT/β-catenin signaling. Our hypothesis was that granulosa cells signal theca cells (TC) via SFRP4, DKK1, RSPO1 and WNT secretion to regulate TC differentiation and proliferation. Therefore, in vitro experiments were conducted to study the effects of WNT family member 3A (WNT3A), WNT5A, RSPO1, DKK1, insulin-like growth factor 1 (IGF1), bone morphogenetic protein 7 (BMP7), Indian hedgehog (IHH), and fibroblast growth factor-9 (FGF9) on bovine TC proliferation and steroidogenesis. Theca cells of large (8 to 20 mm) and small (3 to 6 mm) follicles were collected from bovine ovaries, TC monolayers were established in vitro, and treated with various doses of recombinant human WNT3A, WNT5A, RSPO1, DKK1, IGF1, FGF9, BMP7, IHH and/or ovine LH in serum-free medium for 48 h. In experiment 1 using LH-treated TC, IGF1, IHH and WNT3A increased (P < 0.05) cell numbers and androstenedione production, whereas WNT3A and BMP7 inhibited (P < 0.05) progesterone production. In experiment 2, FGF9 blocked (P < 0.05) the WNT3A-induced increase in androstenedione production in LH plus IGF1-treated TC. In experiment 3, RSPO1 further increased (P < 0.05) LH plus IGF1-induced progesterone and androstenedione production. In experiment 4, SFRP4 and DKK1 alone had no significant effect on TC proliferation or progesterone production of large-follicle TC, but both blocked the inhibitory effect of WNT5A on androstenedione production. In contrast, DKK1 alone inhibited (P < 0.05) small-follicle TC androstenedione production whereas SFRP4 was without effect. We conclude that the ovarian TC WNT system is functional in cattle, with WNT3A increasing proliferation and androstenedione production of TC.

Mapping of a mouse mammary tumor virus integration site by retroviral LTR—arbitrary polymerase chain reaction

1998 ◽  
Vol 54 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Claus Casper ◽  
Christine Leib-Mösch ◽  
Brian Salmons ◽  
Walter H Günzburg ◽  
Gaby Baumann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mouse Mammary Tumor Virus ◽  
Integration Site ◽  
Chain Reaction ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Polymerase Chain ◽  
Virus Integration ◽  
Virus Integration Site

scholarly journals Association of the Gene Encoding Wingless-Type Mammary Tumor Virus Integration-Site Family Member 5B (WNT5B) with Type 2 Diabetes

2004 ◽  
Vol 75 (5) ◽  
pp. 832-843 ◽  
Author(s):  
Akio Kanazawa ◽  
Syuuichi Tsukada ◽  
Akihiro Sekine ◽  
Tatsuhiko Tsunoda ◽  
Atsushi Takahashi ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Family Member ◽  
Mammary Tumor ◽  
Integration Site ◽  
Gene Encoding ◽  
Mammary Tumor Virus ◽  
Tumor Virus ◽  
Virus Integration ◽  
Virus Integration Site

scholarly journals A New Common Integration Site, Int7, for the Mouse Mammary Tumor Virus in Mouse Mammary Tumors Identifies a Gene Whose Product Has Furin-Like and Thrombospondin-Like Sequences

2005 ◽  
Vol 79 (15) ◽  
pp. 10093-10096 ◽  
Author(s):  
William Lowther ◽  
Korah Wiley ◽  
Gilbert H. Smith ◽  
Robert Callahan
Keyword(s):  
Mammary Tumor ◽  
Transcription Initiation ◽  
Mouse Mammary Tumor Virus ◽  
Integration Site ◽  
Mammary Tumors ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumors ◽  
Tumor Virus ◽  
Common Integration Site

ABSTRACT A novel common integration site for the mouse mammary tumor virus (MMTV) was identified (designated Int7) in five independently arising mouse mammary tumors. The insertion sites all cluster within a 1-kb region that is 2 to 3 kb 5′ of the transcription initiation site of a gene, 2610028F08RIK, whose gene product contains furin-like and thrombospondin-like sequences. Expression of Int7 is normally very low or silent during various stages of mammary gland development, but MMTV integration at this site results in the activation of high steady-state levels of expression of the gene. These five tumors were also found to have two or three additional viral insertions, which in each case occurred flanking a member of either the Wnt and/or FGF gene family. Reverse transcriptase PCR results demonstrated that each of the viral insertions led to elevated expression of the presumed target flanking genes.

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