0852 Effect of cortisol on mammary epithelial cell Bax and Bcl-2 gene expression at lactation peak of goats

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 410-410
Author(s):  
G. F. Bomfim
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial

scholarly journals Gene Expression Profile in Response to Doxorubicin–Rapamycin Combined Treatment of HER-2–Overexpressing Human Mammary Epithelial Cell Lines

2011 ◽  
Vol 11 (2) ◽  
pp. 464-474 ◽  
Author(s):  
Adriana Priscila Trapé ◽  
Maria Lucia Hirata Katayama ◽  
Rosimeire Aparecida Roela ◽  
Helena Brentani ◽  
Graziela Rosa Ravacci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Gene Expression Profile ◽  
Mammary Epithelial Cell ◽  
Combined Treatment ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Lines ◽  
Human Mammary Epithelial ◽  
Her 2

scholarly journals Glucocorticoid Receptor Activation Signals through Forkhead Transcription Factor 3a in Breast Cancer Cells

2006 ◽  
Vol 20 (10) ◽  
pp. 2304-2314 ◽  
Author(s):  
Wei Wu ◽  
Min Zou ◽  
Deanna R. Brickley ◽  
Travis Pew ◽  
Suzanne D. Conzen
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Transcription Factor ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Breast Cancer Cells ◽  
Receptor Activation ◽  
Mammary Epithelial ◽  
Forkhead Transcription Factor ◽  
Igfbp 3

Abstract Activation of the glucocorticoid receptor (GR) plays a critical role in the stress response of virtually all cell types. Despite recent advances in large-scale genomic and proteomic data acquisition, identification of physiologically relevant molecular events downstream of nuclear hormone receptor activation remains challenging. By analyzing gene expression changes 30 min after dexamethasone (Dex) treatment, we previously found that immediate induction of serum and glucocorticoid-regulated kinase-1 (SGK-1) expression is required for GR-mediated mammary epithelial cell survival signaling. We now report that activation of the GR mediates Forkhead transcription factor 3a (FOXO3a) phosphorylation and inactivation in mammary epithelial cells. GR-mediated induction of SGK-1 expression is required for FOXO3a inactivation; additional growth factor stimulation is not required. To further explore the gene expression changes that occur downstream of GR-mediated FOXO3a inactivation, we analyzed temporal gene expression data and selected GR-down-regulated genes containing core FOXO3a binding motifs in their proximal promoters. This approach revealed several previously unrecognized transcriptional target genes of FOXO3a, including IGF binding protein-3 (IGFBP-3). Endogenous IGFBP-3 expression was confirmed to be dependent on the GR-SGK-1-FOXO3a signaling pathway. Moreover, GR activation decreased FOXO3a-induced apoptosis in SK-BR-3 breast cancer cells. Collectively, our data suggest that GR-mediated FOXO3a inactivation is an important mechanism contributing to glucocorticoid-mediated mammary epithelial cell survival.

scholarly journals Modulation of ATM gene expression in a murine mammary epithelial cell line

2000 ◽  
Vol 2 (S1) ◽  
Author(s):  
GV Vutskits ◽  
P Salmon ◽  
JV Soriano ◽  
C Cudre-Mauroux ◽  
D Trono ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Atm Gene

Effect of growth factors and lactogenic hormones on expression of plasminogen activator-related genes and cell proliferation in a bovine mammary epithelial cell line

2011 ◽  
Vol 78 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Georgios Theodorou ◽  
Chiara Pecorini ◽  
Raffaella Rebucci ◽  
Francesca Saccone ◽  
Christina Lecchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Growth Factors ◽  
Epithelial Cell ◽  
Plasminogen Activator ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Bovine Mammary Epithelial Cell ◽  
Pai 1

There is conflicting evidence in the literature as to whether up-regulation of urokinase plasminogen activator (u-PA) expression is related to bovine mammary epithelial cell growth. The role of u-PA receptor (u-PAR) and that of the plasminogen activator inhibitors type 1 and type 2 (PAI-1 and PAI-2) in bovine mammary epithelial cell proliferation is not known. The effect of growth factors and various hormones known to affect mammary function on expression of u-PA, u-PAR, PAI-1, PAI-2 and cell proliferation using the BME-UV1 bovine mammary epithelial cell line was examined. Cell proliferation was measured using the MTT assay and direct cell enumeration. Results showed that both IGF-1 and EGF increased cell proliferation but EGF was a more potent mitogen than IGF-1. Furthermore, IGF-1 increased by 2-fold expression of both u-PA and u-PAR while EGF increased by 3·8-fold the expression of only u-PAR. Both growth factors had no effect on expression of PAI-1 and PAI-2. In a manner consistent with changes in gene expression, EGF and to a lesser extent IGF-1 up-regulated total cell associated, membrane-bound and secreted u-PA activity. Thus, a strong correlation exists between u-PAR gene expression along with the activity of u-PA present on cell membranes and cell proliferation. Dexamethasone, prolactin and surprisingly insulin had no effect on cell proliferation. Dexamethasone alone and when combined with insulin or prolactin up-regulated gene expression of both PAI- and PAI-2 but not that of u-PA and u-PAR. Decreased total cell-associated, membrane-bound and secreted u-PA activity was detected in cells cultured in the presence of dexamethasone when combined with insulin or prolactin. However no such effect was observed in the presence of dexamethasone alone. Thus, dexamethasone acting synergistically with prolactin or insulin inhibits the activation of the plasmin-plasminogen system but this inhibition is not correlated with any changes in cell proliferation.

scholarly journals Prolactin and ErbB4/HER4 Signaling Interact via Janus Kinase 2 to Induce Mammary Epithelial Cell Gene Expression Differentiation

2008 ◽  
Vol 22 (10) ◽  
pp. 2307-2321 ◽  
Author(s):  
Rebecca S. Muraoka-Cook ◽  
Melissa Sandahl ◽  
Debra Hunter ◽  
Leah Miraglia ◽  
H. Shelton Earp
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Janus Kinase ◽  
Janus Kinase 2 ◽  
Cell Gene Expression ◽  
Cell Gene
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