0850 Consumption of endophyte-infected fescue seed during the dry period and lactation affects mammary gland gene expression in dairy cows

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 409-409
Author(s):  
R. L. Baldwin ◽  
C. Li ◽  
D. M. Bickhart ◽  
C. M. Evock-Clover ◽  
P. Grossi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Dry Period

445 Toxy-Nil and Unike Plus modulate differences in gene expression of milk somatic cells isolated from mammary gland of lactating dairy cows fed aflatoxin B1

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 218-219
Author(s):  
R. O. Rodrigues ◽  
R. O. Rodrigues ◽  
D. R. Ledoux ◽  
G. E. Rottinghaus ◽  
R. Borutova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Aflatoxin B1 ◽  
Somatic Cells ◽  
Lactating Dairy Cows ◽  
Milk Somatic Cells

scholarly journals Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Atsushi Watanabe ◽  
Jiro Hirota ◽  
Shinya Shimizu ◽  
Shigeki Inumaru ◽  
Kazuhiro Kimura
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Inflammatory Responses ◽  
Clinical Mastitis ◽  
Interleukin 8 ◽  
Dry Period ◽  
Leukocyte Elastase ◽  
Inflammatory Reactions ◽  
Post Infusion

A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot formation from 1 to 28 days post infusion (PI). In the mammary secretions of the mastitic glands, high levels of IL-8 were sustained from 8 hours to 28 days PI, peaking at 1–3 days PI. The levels of leukocyte-derived elastase and inflammatory 22 and 23 kDa lactoferrin derived peptides (LDP) were also increased in the mammary secretions from the mastitic glands. In addition to the experimentally induced mastitis, the mammary secretions from the glands of cattle with spontaneousStaphylococcus aureusdry-period mastitis displayed milk clot formations and significant increases in their levels of PMNL counts, elastase, LDP, and IL-8, compared with those of the mammary secretions from the uninfected glands. These results suggest that after an intramammary infusion of IL-8 has elicited inflammatory responses, it induces the prolonged secretion of elastase, inflammatory LDP, and IL-8, and that long-lasting IL-8-induced inflammatory reactions are involved in the pathogenesis ofS. aureusdry-period mastitis.

Functional analysis of the dairy cow mammary transcriptome between early lactation and mid-dry period

2019 ◽  
Vol 86 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Ye Lin ◽  
He Lv ◽  
Minghui Jiang ◽  
Jinyu Zhou ◽  
Shuyuan Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cows ◽  
Digital Gene Expression ◽  
Mammary Glands ◽  
Differentially Expressed ◽  
Mammary Tissue ◽  
Early Lactation ◽  
Gene Ontology Analysis ◽  
Dry Period ◽  
Molecular Events

AbstractIn this research communication we used digital gene expression (DGE) analysis to identify differences in gene expression in the mammary glands of dairy cows between early lactation and the mid-dry period. A total of 741 genes were identified as being differentially expressed by DGE analysis. Compared with their expression in dry cows, 214 genes were up-regulated and 527 genes were down-regulated in lactating cow mammary glands. Gene Ontology analysis showed that lactation was supported by increased gene expression related to metabolic processes and nutrient transport and was associated with decreased gene expression related to cell proliferation. Pathway mapping using the Kyoto Encyclopedia of Genes and Genomes showed that 579 differentially expressed genes had pathway annotations related to 204 pathways. Metabolic pathway-related genes were the most significantly enriched. Genes and pathways identified by the present study provide insights into molecular events that occur in the mammary gland between early lactation and mid-dry period, which can be used to facilitate further investigation of the mechanisms underlying lactation and mammary tissue remodeling in dairy cows.

scholarly journals Supplements of vitamins B9 and B12 affect hepatic and mammary gland gene expression profiles in lactating dairy cows

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Bazoumana Ouattara ◽  
Nathalie Bissonnette ◽  
Melissa Duplessis ◽  
Christiane L. Girard
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Lactating Dairy Cows

scholarly journals Changes in gene expression in the rumen and colon epithelia during the dry period through lactation of dairy cows and effects of live yeast supplementation

2018 ◽  
Vol 101 (3) ◽  
pp. 2631-2640 ◽  
Author(s):  
A. Bach ◽  
I. Guasch ◽  
G. Elcoso ◽  
F. Chaucheyras-Durand ◽  
M. Castex ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cows ◽  
Dry Period ◽  
Live Yeast

Prepartal dietary energy alters transcriptional adaptations of the liver and subcutaneous adipose tissue of dairy cows during the transition period

2014 ◽  
Vol 46 (9) ◽  
pp. 328-337 ◽  
Author(s):  
S. Selim ◽  
S. Salin ◽  
J. Taponen ◽  
A. Vanhatalo ◽  
T. Kokkonen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Dairy Cows ◽  
Subcutaneous Adipose Tissue ◽  
Transition Period ◽  
Live Weight ◽  
High Energy ◽  
Dry Period ◽  
Nonesterified Fatty Acids ◽  
Subcutaneous Adipose

Overfeeding during the dry period may predispose cows to increased insulin resistance (IR) with enhanced postpartum lipolysis. We studied gene expression in the liver and subcutaneous adipose tissue (SAT) of 16 Finnish Ayrshire dairy cows fed either a controlled energy diet [Con, 99 MJ/day metabolizable energy (ME)] during the last 6 wk of the dry period or high-energy diet (High, 141 MJ/day ME) for the first 3 wk and then gradually decreasing energy allowance during 3 wk to 99 MJ/day ME before the expected parturition. Tissue biopsies were collected at −10, 1, and 9 days, and blood samples at −10, 1, and 7 days relative to parturition. Overfed cows had greater dry matter, crude protein, and ME intakes and ME balance before parturition. Daily milk yield, live weight, and body condition score were not different between treatments. The High cows tended to have greater plasma insulin and lower glucagon/insulin ratio compared with Con cows. No differences in circulating glucose, glucagon, nonesterified fatty acids and β-hydroxybutyrate concentrations, and hepatic triglyceride contents were observed between treatments. Overfeeding compared with Con resulted in lower CPT1A and PCK1 and a tendency for lower G6PC and PC expression in the liver. The High group tended to have lower RETN expression in SAT than Con. No other effects of overfeeding on the expression of genes related to IR in SAT were observed. In conclusion, overfeeding energy prepartum may have compromised hepatic gluconeogenic capacity and slightly affected IR in SAT based on gene expression.

scholarly journals Short Time-Series Expression Transcriptome Data Reveal the Gene Expression Patterns of Dairy Cow Mammary Gland as Milk Yield Decreased Process

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 942
Author(s):  
Yongliang Fan ◽  
Ziyin Han ◽  
Xubin Lu ◽  
Abdelaziz Adam Idriss Arbab ◽  
Mudasir Nazar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Function Analysis ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Short Time Series ◽  
Series Expression ◽  
Short Time

The existing research on dairy cow mammary gland genes is extensive, but there have been few reports about dynamic changes in dairy cow mammary gland genes as milk yield decrease. For the first time, transcriptome analysis based on short time-series expression miner (STEM) and histological observations were performed using the Holstein dairy cow mammary gland to explore gene expression patterns in this process of decrease (at peak, mid-, and late lactation). Histological observations suggested that the number of mammary acinous cells at peak/mid-lactation was significantly higher than that at mid-/late lactation, and the lipid droplets area secreted by dairy cows was almost unaltered across the three stages of lactation (p > 0.05). Totals of 882 and 1439 genes were differentially expressed at mid- and late lactation, respectively, compared to peak lactation. Function analysis showed that differentially expressed genes (DEGs) were mainly related to apoptosis and energy metabolism (fold change ≥ 2 or fold change ≤ 0.5, p-value ≤ 0.05). Transcriptome analysis based on STEM identified 16 profiles of differential gene expression patterns, including 5 significant profiles (false discovery rate, FDR ≤ 0.05). Function analysis revealed DEGs involved in milk fat synthesis were downregulated in Profile 0 and DEGs in Profile 12 associated with protein synthesis. These findings provide a foundation for future studies on the molecular mechanisms underlying mammary gland development in dairy cows.

Sign in / Sign up

Export Citation Format

Share Document