0705 Effect of milk protein composition on in vivo gastric digestion of a model infant formula

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 337-338
Author(s):  
N. Rafiee Tari ◽  
M. Z. Fan ◽  
M. Corredig
Keyword(s):  
Infant Formula ◽  
Milk Protein ◽  
Protein Composition ◽  
Gastric Digestion

scholarly journals Effect of milk protein composition of a model infant formula on the physicochemical properties of in vivo gastric digestates

2018 ◽  
Vol 101 (4) ◽  
pp. 2851-2861 ◽  
Author(s):  
N. Rafiee Tari ◽  
M.Z. Fan ◽  
T. Archbold ◽  
E. Kristo ◽  
A. Guri ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Infant Formula ◽  
Milk Protein ◽  
Protein Composition

In vitro dynamic gastric digestion of soya protein/milk protein blended beverages: influence of protein composition and co-processing

2021 ◽  
Vol 12 (6) ◽  
pp. 2605-2616
Author(s):  
Teresa Francis Wegrzyn ◽  
Alejandra Acevedo-Fani ◽  
Simon M. Loveday ◽  
Harjinder Singh
Keyword(s):  
Whey Protein ◽  
Milk Protein ◽  
Protein Composition ◽  
Soya Protein ◽  
Gastric Digestion ◽  
Heat Treated ◽  
Protein Milk

The gastric digestion behaviours of blended protein beverages containing different ratios of casein, whey protein and soya protein that were heat-treated at 60 °C or 80 °C were investigated using an in vitro dynamic human gastric simulator.

Formula and complementary feeding

2018 ◽  
Author(s):  
John Puntis
Keyword(s):  
Infant Formula ◽  
Soy Protein ◽  
Milk Protein ◽  
Complementary Feeding ◽  
Protein Composition ◽  
Bottle Feeding ◽  
Cow Milk ◽  
International Variation ◽  
Simple Rules ◽  
Nutritional Needs

Some mothers are unable, or choose not, to breastfeed; bottle-feeding carries certain risks that can be minimized by following simple rules. Formula must satisfy all the nutritional needs of an infant and recommendations for the composition of infant formula have been adopted in the Codex Alimentarius. The two main types of formula differ in protein composition (whey or casein predominant). Follow-on formula are designed for infants from 6–12 months of age. Soy protein is an alternative to cow milk protein, but because of its high phytoestrogen content, should not be used before 6 months. Unmodified cow’s milk as the main drink before 12 months of age is associated with iron deficiency. ‘Complementary feeding’ embraces all solid and liquid feeds other than breast milk and infant formula. There is considerable international variation in practice with regard to introduction of complementary feeds, but in general this should not be before 17 weeks, and not after 26 weeks.

scholarly journals Molecular Characterization of Dendritic Cell-Derived Exosomes

1999 ◽  
Vol 147 (3) ◽  
pp. 599-610 ◽  
Author(s):  
Clotilde Théry ◽  
Armelle Regnault ◽  
Jérôme Garin ◽  
Joseph Wolfers ◽  
Laurence Zitvogel ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Heat Shock ◽  
Immune Responses ◽  
Milk Fat ◽  
Protein Composition ◽  
Antitumor Effects ◽  
Peripheral Tissues ◽  
Peptide Mass ◽  
Dc Maturation

Exosomes are membrane vesicles secreted by hematopoietic cells upon fusion of late multivesicular endosomes with the plasma membrane. Dendritic cell (DC)-derived exosomes induce potent antitumor immune responses in mice, resulting in the regression of established tumors (Zitvogel, L., A. Regnault, A. Lozier, J. Wolfers, C. Flament, D. Tenza, P. Ricciardi-Castagnoli, G. Raposo, and S. Amigorena. 1998. Nat. Med. 4:594–600). To unravel the molecular basis of exosome-induced immune stimulation, we now analyze the regulation of their production during DC maturation and characterize extensively their protein composition by peptide mass mapping. Exosomes contain several cytosolic proteins (including annexin II, heat shock cognate protein hsc73, and heteromeric G protein Gi2α), as well as different integral or peripherally associated membrane proteins (major histocompatiblity complex class II, Mac-1 integrin, CD9, milk fat globule-EGF-factor VIII [MFG-E8]). MFG-E8, the major exosomal component, binds integrins expressed by DCs and macrophages, suggesting that it may be involved in exosome targeting to these professional antigen-presenting cells. Another exosome component is hsc73, a cytosolic heat shock protein (hsp) also present in DC endocytic compartments. hsc73 was shown to induce antitumor immune responses in vivo, and therefore could be involved in the exosome's potent antitumor effects. Finally, exosome production is downregulated upon DC maturation, indicating that in vivo, exosomes are produced by immature DCs in peripheral tissues. Thus, DC-derived exosomes accumulate a defined subset of cellular proteins reflecting their endosomal biogenesis and accounting for their biological function.

A multi-scale approach to identify the role of heat treatment, milk protein composition and starter culture on the gel formation and the texture defects of acid milk gel

2018 ◽  
Vol 85 ◽  
pp. 299-310 ◽  
Author(s):  
An Thi-Binh Nguyen ◽  
Michaël Nigen ◽  
Luciana Jimenez ◽  
Hassina Ait-Abderahim ◽  
Charles Cunault ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Milk Protein ◽  
Starter Culture ◽  
Protein Composition ◽  
Gel Formation ◽  
Multi Scale ◽  
Acid Milk Gel

The effect of level and type of supplement offered to grazing dairy cows on herbage intake, animal performance and rumen fermentation characteristics

2003 ◽  
Vol 76 (3) ◽  
pp. 439-454 ◽  
Author(s):  
H. J. Sayers ◽  
C. S. Mayne ◽  
C. G. Bartram
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Milk Protein ◽  
Protein Composition ◽  
Experimental Period ◽  
Yield Response ◽  
Animal Performance ◽  
Two Phase ◽  
Grazed Pasture ◽  
Herbage Intake

AbstractA study was undertaken to examine the effect of level and type of supplement and changes in the chemical composition of herbage through the grazing season on herbage intake, animal performance and rumen characteristics with high-yielding dairy cows. Thirty-two high genetic merit dairy cows were allocated to one of four treatments in a two-phase change-over design experiment. The four treatments involved offering either high-fibre (HF) or high-starch (HS) supplements at either 5 or 10 kg dry matter (DM) per day. Animals rotationally grazed perennial ryegrass swards offered a daily herbage allowance of 23 kg DM. High levels of animal performance were maintained throughout the study with mean milk yields over the 21-week experimental period of 35·5 and 37·2 kg per cow per day on the 5- and 10-kg treatments respectively. Supplement type had no significant effect on herbage intake. Increasing the level of supplementation reduced herbage intake by 0·49 and 0·46 kg herbage DM per kg supplement DM and increased milk yield, with a milk yield response of 0·55 and 0·65 kg/kg supplement DM offered in phases 1 and 2. Supplement type had no significant effect on milk yield and milk butterfat content. However, in both phases 1 and 2, milk protein content was significantly higher with cows offered the HS supplement compared with the HF supplement, this being particularly evident at the higher level of supplementation. Supplement type also had marked effects on milk protein composition. These results indicate that high levels of performance can be achieved with dairy cows on grazed pasture with moderate levels of supplementation.

scholarly journals Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma

NAR Cancer ◽  
2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Verónica Freire-Benéitez ◽  
Nicola Pomella ◽  
Thomas O Millner ◽  
Anaëlle A Dumas ◽  
Maria Victoria Niklison-Chirou ◽  
...  
Keyword(s):  
Chromatin Immunoprecipitation ◽  
Protein Composition ◽  
Mrna Splicing ◽  
Cholesterol Transport ◽  
Normal Brain ◽  
Regulatory Functions ◽  
Polycomb Repressive Complex 1 ◽  
Normal Brain Development

Abstract Glioblastoma (GBM) is the most common and aggressive intrinsic brain tumour in adults. Epigenetic mechanisms controlling normal brain development are often dysregulated in GBM. Among these, BMI1, a structural component of the Polycomb Repressive Complex 1 (PRC1), which promotes the H2AK119ub catalytic activity of Ring1B, is upregulated in GBM and its tumorigenic role has been shown in vitro and in vivo. Here, we have used protein and chromatin immunoprecipitation followed by mass spectrometry (MS) analysis to elucidate the protein composition of PRC1 in GBM and transcriptional silencing of defining interactors in primary patient-derived GIC lines to assess their functional impact on GBM biology. We identify novel regulatory functions in mRNA splicing and cholesterol transport which could represent novel targetable mechanisms in GBM.

scholarly journals A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria

2021 ◽  
Vol 8 ◽  
Author(s):  
Pei Kun Richie Tay ◽  
Pei Yu Lim ◽  
Dave Siak-Wei Ow
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Surface Display ◽  
Bioactive Molecules ◽  
Gastric Digestion ◽  
Functional Protein ◽  
Fluorescent Reporter ◽  
Surface Conditions ◽  
Bacterial Surface

Lactic acid bacteria (LAB) are a group of gut commensals increasingly recognized for their potential to deliver bioactive molecules in vivo. The delivery of therapeutic proteins, in particular, can be achieved by anchoring them to the bacterial surface, and various anchoring domains have been described for this application. Here, we investigated a new cell anchoring domain (CAD4a) isolated from a Lactobacillus protein, containing repeats of a SH3_5 motif that binds non-covalently to peptidoglycan in the LAB cell wall. Using a fluorescent reporter, we showed that C-terminal CAD4a bound Lactobacillus fermentum selectively out of a panel of LAB strains, and cell anchoring was uniform across the cell surface. Conditions affecting CAD4a anchoring were studied, including temperature, pH, salt concentration, and bacterial growth phase. Quantitative analysis showed that CAD4a allowed display of 105 molecules of monomeric protein per cell. We demonstrated the surface display of a functional protein with superoxide dismutase (SOD), an antioxidant enzyme potentially useful for treating gut inflammation. SOD displayed on cells could be protected from gastric digestion using a polymer matrix. Taken together, our results show the feasibility of using CAD4a as a novel cell anchor for protein surface display on LAB.

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