0512 Identification of protein fractions in ripened American style natural cheese manufactured utilizing recombinant bovine and camel chymosin by capillary electrophoresis

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 246-246 ◽  
Author(s):  
A. C. Biswas ◽  
L. Metzger
Keyword(s):  
Capillary Electrophoresis ◽  
Protein Fractions ◽  
American Style

Separation of the unique proteins of wheat protein fractions by capillary electrophoresis

2000 ◽  
Vol 51 (1) ◽  
pp. S130-S134 ◽  
Author(s):  
E. Scholz ◽  
K. Ganzler ◽  
Sz. Gergely ◽  
A. Salgo
Keyword(s):  
Capillary Electrophoresis ◽  
Protein Fractions ◽  
Wheat Protein ◽  
Wheat Protein Fractions

scholarly journals Analytical Evaluation of the Novel Helena V8 Capillary Electrophoresis System

2013 ◽  
Vol 32 (3) ◽  
pp. 245-249
Author(s):  
Giuseppe Lippi ◽  
Luisita Battistelli ◽  
Arialdo Vernocchi ◽  
Michele Mussap
Keyword(s):  
Capillary Electrophoresis ◽  
Biological Fluids ◽  
Protein Fractions ◽  
The Novel ◽  
Medical Systems ◽  
Suitable Alternative ◽  
Electrophoresis System ◽  
The Mean ◽  
Beta 2 ◽  
Monoclonal Component

Summary Background: Capillary electrophoresis (CE) is used for the screening of protein abnormalities in serum and other biological fluids. Since the Helena V8 CE system has been recently commercialized, the aim of this study was to assess its analytical performance under routine laboratory conditions. Methods: The evaluation of Helena V8 (Medical Systems S.p.A., Genova, Italy) for protein fractions (i.e., albumin, alpha-1, alpha-2, beta-1, beta-2, gamma, albumin/globulin ratio [A/G]) and monoclonal component (MC) quantification included imprecision studies, linearity, and comparison with Sebia Capillarys (Sebia, Evry Cedex, France), which was considered the reference analyzer. Results: The imprecision of Helena V8 on all parameters was comprised between 0.8% and 15.2%; the linearity was excellent up to an MC value of 14.5% (r=1.00). The comparison studies showed excellent Spearman’s correlations with Sebia Capillarys, with coefficients comprised between 0.84 and 0.99 (all p<0.001). The mean bias of Helena V8 versus Sebia Capillarys was -0.02% for G/A, -0.2% for albumin, 2.1% for alpha-1, 0.1% for alpha-2, 0.3% for beta-1, -0.2% for beta-2, -2.2% for gamma and 1 g/L for MC. Conclusions: The results of this evaluation attest that Helena V8 is a suitable alternative for routine separation and quantification of protein fractions and monoclonal component.

Reference intervals for electrophoretograms obtained by capillary electrophoresis of dialyzed urine from healthy dogs

2021 ◽  
pp. 104063872110201
Author(s):  
Paula F. Navarro ◽  
Laura Gil ◽  
Germán Martín ◽  
Salceda Fernández-Barredo
Keyword(s):  
Capillary Electrophoresis ◽  
Nonparametric Methods ◽  
Reference Intervals ◽  
Protein Fractions ◽  
Urine Samples ◽  
Tubular Damage ◽  
Urine Protein ◽  
Electrophoretic Patterns ◽  
Healthy Dogs

Electrophoresis of urine to evaluate protein fractions in dogs with proteinuria to differentiate glomerular from tubular damage has increased in recent years; however, capillary electrophoresis (CE) of urine has not been reported in a study of > 40 healthy animals, to our knowledge. We aimed to establish reference intervals (RIs) for the urine protein fractions obtained by CE of urine from healthy dogs. We obtained urine samples from 123 clinically healthy dogs of both sexes between December 2016 and April 2019; urine was frozen until CE was performed. The electrophoretic patterns obtained were divided into 5 protein fractions, and RIs were established in percentages and absolute values using nonparametric methods. RIs were obtained for the fractions (F) as follows: 5.5 to 56.2% for F1, 3.2 to 16.5% for F2, 3.5 to 16.2% for F3, 17.8 to 69.8% for F4, and 5.1 to 23.9% for F5. These RIs obtained by CE might be useful clinically as a basis for comparison with pathologic samples. Age was a statistically significant factor for F2 ( p = 0.01) and F3 ( p = 0.02), and sex was a statistically significant factor for F1 ( p = 0.03).

Determination of cows' milk in goats' milk and cheese by capillary electrophoresis of the whey protein fractions

1999 ◽  
Vol 846 (1-2) ◽  
pp. 135-141 ◽  
Author(s):  
Giampaolo Cartoni ◽  
Franco Coccioli ◽  
Renata Jasionowska ◽  
Maurizio Masci
Keyword(s):  
Capillary Electrophoresis ◽  
Whey Protein ◽  
Protein Fractions
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