0348 The polymorphisms of Toll-like receptor 4 gene influences milk production traits in Chinese Holstein cows

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 167-168 ◽  
Author(s):  
X. Zhu ◽  
M. Wang ◽  
S. Xing ◽  
Z. Yang ◽  
Y. Mao
Keyword(s):  
Milk Production ◽  
Holstein Cows ◽  
Production Traits ◽  
Toll Like Receptor ◽  
Milk Production Traits ◽  
Toll Like Receptor 4 ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows

2018 ◽  
Vol 85 (4) ◽  
pp. 407-411 ◽  
Author(s):  
Mengqi Wang ◽  
Hailiang Song ◽  
Xiaorui Zhu ◽  
Shiyu Xing ◽  
Meirong Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Milk Production ◽  
Gene Polymorphisms ◽  
Holstein Cows ◽  
Production Traits ◽  
Toll Like Receptor ◽  
Milk Production Traits ◽  
Toll Like Receptor 4 ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

The research reported in this Research Communication aimed to describe the influence of Toll-like receptor 4 gene polymorphisms on milk production traits in Chinese Holstein cows. Toll-like receptor 4 (TLR4) is an important member of the toll-like receptor gene family that is widely found in various organisms. Since TLR4 can identify molecular patterns from various pathogenic microorganisms and induce natural and acquired immunity, it plays an important role in disease resistance in dairy cows. Two single nucleotide polymorphisms (SNPs) of TLR4 (c.−226 G > C and c.2021 C > T) that were previously found to be associated with health traits were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) for Chinese Holstein cows (n = 866). The associations between SNPs or their haplotypes and milk production traits and somatic cell count were analyzed by the generalized linear model procedure of Statistics Analysis System software (SAS). The c.−226 G > C and c.2021 C > T showed low linkage disequilibrium (r2 = 0·192). There was no association between these two SNPs and SCC, but significant effects were found for SNP c.−226 G > C on test-day milk yield, fat content, protein content, and total solid and milk urea nitrogen (P < 0·05), and SNP c.2021 C > T and the SNP haplotypes on test-day milk yield, fat content, protein content, lactose content and total solids (P < 0·05). The software MatInspector revealed that c.−226 G > C was located within several potential transcription factor binding sites, including transcription factor AP-2. The polymorphisms c.−226 G > C and c.2021 C > T had significant effects on the milk production for Chinese Holstein, and these SNP could be used for molecular marker-assisted selection of milk production.

scholarly journals Polymorphisms in Fatty Acid Desaturase 2 Gene Are Associated with Milk Production Traits in Chinese Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 671 ◽  
Author(s):  
Mingxun Li ◽  
Qisong Gao ◽  
Mengqi Wang ◽  
Yan Liang ◽  
Yujia Sun ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Production ◽  
Milk Yield ◽  
Fatty Acid Desaturase ◽  
Holstein Cows ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows ◽  
Fatty Acid Desaturase 2

This study investigated the single nucleotide polymorphisms (SNPs) of Fatty acid desaturase 2 (FADS2) gene and further explored their genetic effects on conventionally collected milk production traits in Chinese Holstein cows using 18,264 test-day records of 841 cows. One missense mutation c. 908 C > T (SNP site in the complementary DNA sequence), which caused an amino acid change from alanine to valine (294Ala > Val), and two 3’ untranslated region (UTR) SNPs, c.1571 G > A and c.2776 A > G were finally identified. The SNP c.908 C > T was significantly associated with test-day milk yield, fat percentage and 305-day milk, fat and protein yield. In particular, the T allele of the SNP c.908 C > T showed a significant association with decreased somatic cell score (SCS) in the investigated population. Significant relationship between the SNP c.1571 G > A and 305-day milk yield showed that genotype GG was linked to the highest milk yield. Substituting the allele G for A at the c.2776 A > G locus resulted in a decrease of protein percentage. Our results demonstrated that FADS2 was an interesting candidate for selection to increase milk production and improve resistance against mastitis.

scholarly journals Polymorphisms of the ACSL1 Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2282
Author(s):  
Yan Liang ◽  
Qisong Gao ◽  
Qiang Zhang ◽  
Abdelaziz Adam Idriss Arbab ◽  
Mingxun Li ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Milk Production ◽  
Somatic Cell Score ◽  
Holstein Cows ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chain Acyl ◽  
Chinese Holstein Cows ◽  
Long Chain

Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (ACSL1) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in ACSL1 on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine ACSL1 gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (p < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (p < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (p < 0.01), and also TDMY (p < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (p < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (p < 0.01) and significantly influenced PC (p < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (p < 0.05). In summary, the pleiotropic effects of bovine ACSL1 for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.

scholarly journals Associations between variants of the HALgene and milk production traits in Chinese Holstein cows

BMC Genetics ◽  
2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Haifei Wang ◽  
Li Jiang ◽  
Wenwen Wang ◽  
Shengli Zhang ◽  
Zongjun Yin ◽  
...  
Keyword(s):  
Milk Production ◽  
Holstein Cows ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

scholarly journals Polymorphism in the promoter of <i>TLR4</i> gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

2014 ◽  
Vol 57 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zhixiong Li ◽  
Huilin Zhang ◽  
Hongliang Wang ◽  
Ling Chen ◽  
Lijun Wang ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Milk Production ◽  
Somatic Cell Score ◽  
Upstream Region ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows ◽  
Tlr4 Gene

Abstract. The objective of this study was to examine the effect of the polymorphism in the promoter of toll-like receptor 4 (TLR4) on mastitis and milk production traits. Two novel single nucleotide polymorphisms (SNPs) G-1539A before 5’-upstream region and G+265C in the 5’-UTR of TLR4 gene were detected, and genetic association analysis was applied on 421 Chinese Holstein cows. The statistical analysis indicated that two SNPs was significantly associated with 305 Days milk yield (P<0.05) and somatic cell score (SCS) respectively (P<0.01). Our results provide evidence that polymorphisms in the promoter of TLR4 are associated with milk production traits and SCS, and may be used as a possible candidate for marker-assisted selection and management in Holstein cattle breeding program.

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