0285 Genome-wide association study of bovine respiratory disease complex in U.S. feedlot cattle

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 135-135
Author(s):  
C. M. Seabury ◽  
H. L. Neibergs ◽  
J. F. Taylor ◽  
J. E. Womack
Keyword(s):  
Association Study ◽  
Respiratory Disease ◽  
Genome Wide Association Study ◽  
Bovine Respiratory Disease ◽  
Feedlot Cattle ◽  
Genome Wide Association ◽  
Disease Complex ◽  
Genome Wide ◽  
Bovine Respiratory Disease Complex

Genome-wide association study of aspirin-exacerbated respiratory disease in a Korean population

2012 ◽  
Vol 132 (3) ◽  
pp. 313-321 ◽  
Author(s):  
Byung Lae Park ◽  
Tae-Hoon Kim ◽  
Jeong-Hyun Kim ◽  
Joon Seol Bae ◽  
Charisse Flerida A. Pasaje ◽  
...  
Keyword(s):  
Association Study ◽  
Respiratory Disease ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Korean Population ◽  
Aspirin Exacerbated Respiratory Disease ◽  
Genome Wide

scholarly journals Closed Genome Sequences and Antibiograms of 16 Pasteurella multocida Isolates from Bovine Respiratory Disease Complex Cases and Apparently Healthy Controls

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Gregory P. Harhay ◽  
Dayna M. Harhay ◽  
James L. Bono ◽  
Timothy P. L. Smith ◽  
Sarah F. Capik ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Bacterial Species ◽  
Bovine Respiratory Disease ◽  
Opportunistic Pathogen ◽  
Feedlot Cattle ◽  
Content Type ◽  
Disease Complex ◽  
Bovine Respiratory Disease Complex ◽  
Apparently Healthy

Pasteurella multocida is an animal-associated Gram-negative member of the Pasteurellaceae family. It is an opportunistic pathogen and is one of the principal bacterial species contributing to bovine respiratory disease complex (BRDC) in feedlot cattle.

scholarly journals Cattle with increased severity of bovine respiratory disease complex exhibit decreased capacity to protect against histone cytotoxicity

2015 ◽  
Vol 93 (4) ◽  
pp. 1841-1849
Author(s):  
J. A. Matera ◽  
B. K. Wilson ◽  
J. A. Hernandez Gifford ◽  
D. L. Step ◽  
C. R. Krehbiel ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Serum Proteins ◽  
Inflammatory Responses ◽  
Animal Cell ◽  
Bovine Respiratory Disease ◽  
Feedlot Cattle ◽  
Protective Capacity ◽  
Disease Complex ◽  
Bovine Respiratory Disease Complex ◽  
Initial Processing

Abstract Bovine respiratory disease complex (BRDC) is the leading cause of morbidity and mortality in feedlot cattle. Significant inflammation and lesions are often observed in lungs of infected cattle. During acute inflammatory responses, histones contribute to mortality in rodents and humans and serum proteins can protect against histone-induced cytotoxicity. We hypothesized that cattle experiencing chronic or fatal cases of BRDC have reduced ability to protect against cytotoxic effects of histones. Serum samples were collected from 66 bull calves at the time of normal feedlot processing procedures. Animals were retrospectively assigned to groups consisting of calves never treated for BRDC (control [CONT]; n = 10), calves treated with antimicrobials once for BRDC (1T; n = 16), calves treated twice for BRDC (2T; n = 13), calves treated 3 times for BRDC (3T; n = 14), or calves treated 4 times for BRDC (4T; n = 13). Samples were also collected each time animals received antimicrobial treatment; animals within a group were further sorted by calves that recovered and calves that died to test histone cytotoxicity. Bovine kidney cells were cultured in duplicate in 96-well plates and exposed to 0 or 50 μg/mL of total histones for 18 h with 1% serum from each animal. Cell viability was assessed by the addition of resazurin for 6 h followed by fluorescent quantification. Fluorescent values from serum alone were subtracted from values obtained for histone treatment for each animal. Serum from CONT, 1T, and 2T at initial processing all exhibited a similar (P > 0.10) response to histone treatment with fluorescent values of –312 ± 557, –1,059 ± 441, and –975 ± 489, respectively. However, 3T and 4T demonstrated an impaired capacity (P < 0.05) to protect against histones (–2,778 ± 471 and –3,026 ± 489) at initial processing when compared to the other groups. When sorted by mortality within group, calves that were treated twice and recovered (–847 ± 331) demonstrated a greater (P < 0.05) protective capacity than calves that were treated twice and died (–2,264 ± 412), indicating that calves that contract BRDC and ultimately die might have reduced protective capacity against histone cytotoxicity. Results suggest that calves that require multiple treatments for BRDC have reduced ability to protect against cytotoxicity of histones. Understanding the primary mechanism responsible for protecting against histone cytotoxicity could lead to improved identification of animals susceptible to severe cases of BRDC, improved focus and use of available resources, or better treatments for severe cases of BRDC.

scholarly journals Genome-wide association study of chronic sputum production implicates loci involved in mucus production and infection

2022 ◽  
Author(s):  
Richard J Packer ◽  
Nick Shrine ◽  
Robert Hall ◽  
Carl Melbourne ◽  
Rebecca Thompson ◽  
...  
Keyword(s):  
Association Study ◽  
Respiratory Disease ◽  
Genome Wide Association Study ◽  
Association Studies ◽  
Genome Wide Association ◽  
Chromosome 11 ◽  
Genome Wide ◽  
Sputum Production ◽  
A Genome ◽  
Wide Range

Background Chronic sputum production impacts on quality of life and is a feature of many respiratory diseases. Identification of the genetic variants associated with chronic sputum production in a disease agnostic sample could improve understanding of its causes and identify new molecular targets for treatment. Methods We conducted a genome-wide association study (GWAS) of chronic sputum production in UK Biobank. Signals meeting genome-wide significance (P<5x10-8) were fine-mapped and putative causal genes identified by gene expression analysis. GWAS of respiratory traits were interrogated to identify whether the signals were driven by existing respiratory disease amongst the cases and variants were further investigated for wider pleiotropic effects using phenome-wide association studies (PheWAS). Findings From a GWAS of 9,714 cases and 48,471 controls, we identified six novel genome-wide significant signals for chronic sputum production including the Human Leukocyte Antigen (HLA) locus, chromosome 11 mucin locus (containing MUC2, MUC5AC and MUC5B) and the FUT2 locus. The mucin locus signal had previously been reported for association with moderate-to-severe asthma. The HLA signal was fine-mapped to an amino-acid change of threonine to arginine (frequency 36.8%) in HLA-DRB1 (HLA-DRB1*03:147). The signal near FUT2 was associated with expression of several genes including FUT2, for which the direction of effect was tissue dependent. Our PheWAS identified a wide range of associations. Interpretation Novel signals at the FUT2 and mucin loci highlight mucin flucosylation as a driver of chronic sputum production even in the absence of diagnosed respiratory disease and provide genetic support for this pathway as a target for therapeutic intervention.

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