scholarly journals Characterization of the High-Density Bacteria in Biological Phosphorus Removal Process by using Buoyant Density Separation

2007 ◽  
Vol 43 (1) ◽  
pp. 51-62
Author(s):  
TAKASHI KONDO ◽  
YOSHITAKA EBIE ◽  
SATOSHI TSUNEDA ◽  
YUHEI INAMORI
Keyword(s):  
Phosphorus Removal ◽  
Buoyant Density ◽  
High Density ◽  
Biological Phosphorus Removal ◽  
Density Separation ◽  
Removal Process ◽  
Biological Phosphorus

Density separation and molecular methods to characterize enhanced biological phosphorus removal system populations

2002 ◽  
Vol 46 (1-2) ◽  
pp. 195-198 ◽  
Author(s):  
A.J. Schuler ◽  
M. Onuki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
High Density ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
High Concentration ◽  
Density Fractions ◽  
Density Separation ◽  
Storage Products ◽  
Biological Phosphorus

A novel approach to the identification of microorganisms that accumulate high density microbial storage products based on density separation, denaturing gradient gel electrophoresis (DGGE), and DNA sequencing was developed and applied to bench and pilot scale enhanced biological phosphorus removal (EBPR) systems. Polyphosphate (PP), glycogen, and polyhydroxyalkanoates (PHAs), are all of higher density than a typical bacterial cell. PP-accumulating organisms (PAOs), the organisms responsible for EBPR, accumulate all three of these storage products. Density separation in a homogenous solution of Percoll produced a high-density biomass fraction with a relatively high concentration of PAOs, as determined by Neisser staining. DNA was extracted from these fractions, amplified, and separated by DGGE. DGGE profiles demonstrated some bacterial strains were present at a greater concentration in the high density fractions than in low density fractions. These strains were considered PAO candidates. 5 of 12 PAO candidates from high density fractions were γ Proteobacteria and only 1 was a β Proteobacterium. 2 PAO candidates were most similar to recently identified γ Proteobacteria sequences obtained by DGGE analysis of a deteriorated benchtop EBPR system.

A metabolic model of the biological phosphorus removal process: II. Validation during start-up conditions

1995 ◽  
Vol 48 (3) ◽  
pp. 234-245 ◽  
Author(s):  
G. J. F. Smolders ◽  
D. J. Bulstra ◽  
R. Jacobs ◽  
M. C. M. van Loosdrecht ◽  
J. J. Heijnen
Keyword(s):  
Phosphorus Removal ◽  
Metabolic Model ◽  
Biological Phosphorus Removal ◽  
Removal Process ◽  
Start Up ◽  
Biological Phosphorus
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