Characterization of NO Production Over Metallic Surfaces in Air Plasmas Using Laser Induced Fluorescence

2016 ◽  
Author(s):  
Luke D. Allen ◽  
Jason M. Meyers ◽  
Douglas G. Fletcher
Keyword(s):  
Laser Induced Fluorescence ◽  
No Production ◽  
Metallic Surfaces ◽  
Air Plasmas

Electrochemical characterization of micro-cracks in polyurethane resin films deposited on metallic surfaces

2016 ◽  
Vol 46 (12) ◽  
pp. 1237-1243 ◽  
Author(s):  
Karun K. Rao ◽  
Molly Ferguson ◽  
Kyle Murphy ◽  
Jean Zhao ◽  
Daniel Lacks ◽  
...  
Keyword(s):  
Electrochemical Characterization ◽  
Metallic Surfaces ◽  
Polyurethane Resin ◽  
Micro Cracks

Isolation and characterization of sesquiterpenes from Arecophila saccharicola YMJ96022401 with NO production inhibitory activity

2013 ◽  
Vol 85 ◽  
pp. 129-136 ◽  
Author(s):  
Lin-Wen Lee ◽  
Guei-Jane Wang ◽  
Mei-Hsiang Lin ◽  
Yu-Min Ju ◽  
Yen-Wen Lin ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
No Production ◽  
Isolation And Characterization

scholarly journals A novel long intergenic non-coding RNA, Nostrill, regulates iNOS gene transcription and neurotoxicity in microglia

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Nicholas W. Mathy ◽  
Olivia Burleigh ◽  
Andrew Kochvar ◽  
Erin R. Whiteford ◽  
Matthew Behrens ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
No Production ◽  
Cortical Tissue ◽  
Non Coding Rna ◽  
Transcriptional Regulatory ◽  
Lncrna Locus ◽  
Rna Immunoprecipitation ◽  
Long Non Coding Rna

Abstract Background Microglia are resident immunocompetent and phagocytic cells in the CNS. Pro-inflammatory microglia, stimulated by microbial signals such as bacterial lipopolysaccharide (LPS), viral RNAs, or inflammatory cytokines, are neurotoxic and associated with pathogenesis of several neurodegenerative diseases. Long non-coding RNAs (lncRNA) are emerging as important tissue-specific regulatory molecules directing cell differentiation and functional states and may help direct proinflammatory responses of microglia. Characterization of lncRNAs upregulated in proinflammatory microglia, such as NR_126553 or 2500002B13Rik, now termed Nostrill (iNOS Transcriptional Regulatory Intergenic LncRNA Locus) increases our understanding of molecular mechanisms in CNS innate immunity. Methods Microglial gene expression array analyses and qRT-PCR were used to identify a novel long intergenic non-coding RNA, Nostrill, upregulated in LPS-stimulated microglial cell lines, LPS-stimulated primary microglia, and LPS-injected mouse cortical tissue. Silencing and overexpression studies, RNA immunoprecipitation, chromatin immunoprecipitation, chromatin isolation by RNA purification assays, and qRT-PCR were used to study the function of this long non-coding RNA in microglia. In vitro assays were used to examine the effects of silencing the novel long non-coding RNA in LPS-stimulated microglia on neurotoxicity. Results We report here characterization of intergenic lncRNA, NR_126553, or 2500002B13Rik now termed Nostrill (iNOS Transcriptional Regulatory Intergenic LncRNA Locus). Nostrill is induced by LPS stimulation in BV2 cells, primary murine microglia, and in cortical tissue of LPS-injected mice. Induction of Nostrill is NF-κB dependent and silencing of Nostrill decreased inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in BV2 and primary microglial cells. Overexpression of Nostrill increased iNOS expression and NO production. RNA immunoprecipitation assays demonstrated that Nostrill is physically associated with NF-κB subunit p65 following LPS stimulation. Silencing of Nostrill significantly reduced NF-κB p65 and RNA polymerase II recruitment to the iNOS promoter and decreased H3K4me3 activating histone modifications at iNOS gene loci. In vitro studies demonstrated that silencing of Nostrill in microglia reduced LPS-stimulated microglial neurotoxicity. Conclusions Our data indicate a new regulatory role of the NF-κB-induced Nostrill and suggest that Nostrill acts as a co-activator of transcription of iNOS resulting in the production of nitric oxide by microglia through modulation of epigenetic chromatin remodeling. Nostrill may be a target for reducing the neurotoxicity associated with iNOS-mediated inflammatory processes in microglia during neurodegeneration.

scholarly journals A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum

Microbiology ◽  
2006 ◽  
Vol 152 (12) ◽  
pp. 3517-3528 ◽  
Author(s):  
Miguel Balado ◽  
Carlos R. Osorio ◽  
Manuel L. Lemos
Keyword(s):  
Gene Cluster ◽  
Vibrio Anguillarum ◽  
No Production ◽  
Genetic Determinants ◽  
Dihydroxybenzoic Acid ◽  
Peptide Synthetase ◽  
Genes Encoding ◽  
In Trans ◽  
Culture Supernatants

Vibrio anguillarum serotype O2 strains produce a catechol siderophore named vanchrobactin, which has been identified as N-[N′-(2,3-dihydroxybenzoyl)-arginyl]-serine. This work describes a chromosomal region that harbours the genetic determinants necessary for the biosynthesis of vanchrobactin. The authors have identified the genes involved in 2,3-dihydroxybenzoic acid (DHBA) biosynthesis (vabA, vabB and vabC) and activation (vabE), and a gene (vabF) encoding a non-ribosomal peptide synthetase, which is putatively involved in the assembly of the siderophore components. Also described are the identification and characterization of genes encoding a putative vanchrobactin exporter (vabS) and a siderophore esterase (vabH). In-frame deletion mutants in vabA, vabB, vabC, vabE, vabF and vabH were impaired for growth under conditions of iron limitation, and the analysis of culture supernatants by chrome azurol-S and cross-feeding assays showed almost no production of siderophores in any of the vabABCEF mutants. In addition, deletion mutations of vabA, vabB and vabC abolished production of DHBA, as assessed by chemical and biological analyses. Complementation of each mutant with the corresponding gene provided in trans confirmed the involvement of this gene cluster in the biosynthesis of DHBA and vanchrobactin in V. anguillarum strain RV22. Based on chemical and genetic data, and on published models for other catechol siderophores, a model for vanchrobactin biosynthesis is proposed.

Laser-Induced Fluorescence Photogrammetry for Dynamic Characterization of Membrane Structures

AIAA Journal ◽  
2004 ◽  
Vol 42 (10) ◽  
pp. 2124-2129 ◽  
Author(s):  
Adrian A. Dorrington ◽  
Thomas W. Jones ◽  
Paul M. Danehy ◽  
Richard S. Pappa
Keyword(s):  
Laser Induced Fluorescence ◽  
Dynamic Characterization ◽  
Membrane Structures
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