scholarly journals In Vivo Electroporation: A Convenient Method for Gene Transfer to Testicular Cells in Mice

1996 ◽  
Vol 67 (11) ◽  
pp. 975-982
Author(s):  
Tatsuo MURAMATSU ◽  
Osamu SHIBATA ◽  
Yasushige OHMORI ◽  
Jun-ichi OKUMURA
Keyword(s):  
Gene Transfer ◽  
Convenient Method ◽  
In Vivo Electroporation ◽  
Testicular Cells

In vivo electroporation enhances plasmid-based gene transfer of basic fibroblast growth factor for the treatment of ischemic limb

2004 ◽  
Vol 120 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Seiji Nishikage ◽  
Hiroyuki Koyama ◽  
Tetsuro Miyata ◽  
Shigeyuki Ishii ◽  
Hirohumi Hamada ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
In Vivo Electroporation ◽  
Ischemic Limb

Direct gene transfer into rat articular cartilage by in vivo electroporation

2003 ◽  
Vol 17 (8) ◽  
pp. 829-835 ◽  
Author(s):  
Lagrent Grossin ◽  
Christel Cournil-Henrionnet ◽  
Lluis M. Mir ◽  
Bertrand Liagre ◽  
Dominique Dumas ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
In Vivo Electroporation ◽  
Direct Gene

Improvement of Flap Necrosis in a Rat Random Skin Flap Model by In Vivo Electroporation-Mediated HGF Gene Transfer

2017 ◽  
Vol 139 (5) ◽  
pp. 1116e-1127e ◽  
Author(s):  
S. Morteza Seyed Jafari ◽  
Maziar Shafighi ◽  
Helmut Beltraminelli ◽  
Thomas Geiser ◽  
Robert E. Hunger ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Skin Flap ◽  
Flap Necrosis ◽  
In Vivo Electroporation

Neuronal Nitric Oxide Synthase Gene Transfer into the Rat Prostate Using In Vivo Electroporation

2010 ◽  
Vol 2 (2) ◽  
pp. 83-87
Author(s):  
Masayuki OTANI ◽  
Masaki YOSHIDA ◽  
Koichi MASUNAGA ◽  
Takashi NAGATA ◽  
Makoto YONO ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Gene Transfer ◽  
Neuronal Nitric Oxide Synthase ◽  
In Vivo Electroporation ◽  
Rat Prostate ◽  
Oxide Synthase

scholarly journals In Vivo Electroporation-Mediated, Intrahepatic Alpha1 Antitrypsin Gene Transfer Reduces Pulmonary Emphysema in Pallid Mice

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 793
Author(s):  
Marco Sutter ◽  
Tiziana Cremona ◽  
Izabela Nita ◽  
Eleonora Cavarra ◽  
Giuseppe Lungarella ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Lung Function ◽  
Pulmonary Emphysema ◽  
Standard Of Care ◽  
In Vivo Electroporation ◽  
Efficient Procedure ◽  
Novel Approach ◽  
Alpha1 Antitrypsin ◽  
Circulating Levels

Rationale: Mutation in the alpha1 antitrypsin (AAT) gene leads to low circulating levels of AAT, which is associated with several disease processes including pulmonary emphysema. The standard of care relies on substitution with plasma-purified AAT. We studied a novel approach to obtain sustained therapeutic levels of circulating AAT using nonviral in vivo electroporation-mediated gene transfer to the liver. Methods: In vivo intrahepatic electroporation-mediated human AAT gene transfer was performed in C57 Bl/6J mice carrying a genetic deficiency of murine AAT (pallid mice) and suffering from pulmonary emphysema. The animals were evaluated for lung function using flexiVent and detailed stereological assessments. Lung neutrophilic burden was assessed. Results: Pallid mice showed morphologically detectable pulmonary emphysema. Thirty days after in vivo electroporation-mediated gene transfer directly aimed at the liver, circulating human AAT was elevated and lung function was significantly improved compared to non-treated pallid mice. Stereological analysis revealed a reduction in pulmonary emphysema. Conclusion: Our data indicate that in vivo intrahepatic electroporation-mediated gene transfer of AAT is a safe and efficient procedure resulting in reduction of pulmonary emphysema in pallid mice.

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