scholarly journals Callus Formation and Plant Regeneration from Root Explant of Gladiolus (Gladiolus × grandiflora hort.).

2005 ◽  
Vol 4 (1) ◽  
pp. 7-10
Author(s):  
Masakazu Kasumi ◽  
Yasumasa Takatsu ◽  
Kazunori Suzuki ◽  
Takeru Gonai ◽  
Mitsuko Nogi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Formation ◽  
Root Explant

Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.)

2009 ◽  
Vol 98 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Yuichi Uno ◽  
Shigeyuki Nakao ◽  
Yumiko Yamai ◽  
Ryohei Koyama ◽  
Michio Kanechi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Transient Expression ◽  
Callus Formation ◽  
Aster Tripolium

scholarly journals Phloroglucinol Mediated Plant Regeneration of Ornithogalum dubium as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 929
Author(s):  
Carloalberto Petti
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Callus Formation ◽  
Total Phenolic ◽  
Naturally Occurring ◽  
Long Term Experiments ◽  
Direct Embryogenesis ◽  
The Media ◽  
Synthetic Hormones

Tissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is susceptible to tissue culture, and often complex media and hormone requirements are needed to achieve successful plant propagations. The availability of new hormones or chemicals acting as hormones are critical to the expansion of tissue culture potentials. Phloroglucinol has been shown to have certain hormone-like properties in a variety of studies. Ornithogalum dubium, an important geophyte species, was used to characterise the potential of phloroglucinol as the sole plant-like hormone in a tissue culture experiment. Tissue culture, plant regeneration, total phenolic and genetic variability were established by applying a variety of methods throughout long-term experiments. Phloroglucinol did induce callus formation and plant regeneration when used as the sole supplement in the media at a rate of 37%, thus demonstrating auxin/cytokines-like properties. Callus formation was of 3 types, friable and cellular, hard and compact, and a mixture of the two. The important finding was that direct somatogenesis did occur albeit more frequently on younger tissue, whereby rates of induction were up to 52%. It is concluded that phloroglucinol acts as a “hormone-like” molecule and can trigger direct embryogenesis without callus formation.

Effects of explants and growth regulators in garlic callus formation and plant regeneration

2006 ◽  
Vol 87 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Gabriela F. Luciani ◽  
Ana K. Mary ◽  
Cecilia Pellegrini ◽  
N. R. Curvetto
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Callus Formation
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