scholarly journals Development of DNA Markers for Ethylene Production during Fruit Ripening in Japanese Pear

2004 ◽  
Vol 3 (4) ◽  
pp. 323-328 ◽  
Author(s):  
Akihiro Itai
Keyword(s):  
Fruit Ripening ◽  
Dna Markers ◽  
Ethylene Production ◽  
Japanese Pear

scholarly journals Genetic dissection of climacteric fruit ripening in a melon population segregating for ripening behavior

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Lara Pereira ◽  
Miguel Santo Domingo ◽  
Valentino Ruggieri ◽  
Jason Argyris ◽  
Michael A. Phillips ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Ethylene Production ◽  
Genome Wide Association Study ◽  
Recombinant Inbred Lines ◽  
Introgression Line ◽  
Complex Trait ◽  
Chromosome 8 ◽  
Genetic Dissection ◽  
Climacteric Fruit ◽  
A Genome

Abstract Melon is as an alternative model to understand fruit ripening due to the coexistence of climacteric and non-climacteric varieties within the same species, allowing the study of the processes that regulate this complex trait with genetic approaches. We phenotyped a population of recombinant inbred lines (RILs), obtained by crossing a climacteric (Védrantais, cantalupensis type) and a non-climcteric variety (Piel de Sapo T111, inodorus type), for traits related to climacteric maturation and ethylene production. Individuals in the RIL population exhibited various combinations of phenotypes that differed in the amount of ethylene produced, the early onset of ethylene production, and other phenotypes associated with ripening. We characterized a major QTL on chromosome 8, ETHQV8.1, which is sufficient to activate climacteric ripening, and other minor QTLs that may modulate the climacteric response. The ETHQV8.1 allele was validated by using two reciprocal introgression line populations generated by crossing Védrantais and Piel de Sapo and analyzing the ETHQV8.1 region in each of the genetic backgrounds. A Genome-wide association study (GWAS) using 211 accessions of the ssp. melo further identified two regions on chromosome 8 associated with the production of aromas, one of these regions overlapping with the 154.1 kb interval containing ETHQV8.1. The ETHQV8.1 region contains several candidate genes that may be related to fruit ripening. This work sheds light into the regulation mechanisms of a complex trait such as fruit ripening.

CHANGES IN FLESH FIRMNESS AND ETHYLENE PRODUCTION OF DIFFERENT PEACH TYPES DURING FRUIT RIPENING

2008 ◽  
pp. 153-159
Author(s):  
Ming-Te Lu ◽  
Chia-Wei Song ◽  
Chao-Chia Huang ◽  
Shyi-Kuan Ou
Keyword(s):  
Fruit Ripening ◽  
Ethylene Production ◽  
Flesh Firmness

scholarly journals Molecular Cloning of β-Galactosidase from Japanese Pear (Pyrus pyrifolia) and its Gene Expression with Fruit Ripening

2001 ◽  
Vol 42 (5) ◽  
pp. 492-498 ◽  
Author(s):  
Akira Tateishi ◽  
Hiroaki Inoue ◽  
Hajime Shiba ◽  
Shohei Yamaki
Keyword(s):  
Gene Expression ◽  
Molecular Cloning ◽  
Fruit Ripening ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Its Gene

scholarly journals Identification of a Gene Encoding Glutamate Decarboxylase Involved in the Postharvest Fruit Ripening Process in Banana

HortScience ◽  
2014 ◽  
Vol 49 (8) ◽  
pp. 1056-1060 ◽  
Author(s):  
Wei Hu ◽  
Ju-Hua Liu ◽  
Xiao-Ying Yang ◽  
Jian-Bin Zhang ◽  
Cai-Hong Jia ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Ethylene Production ◽  
Glutamate Decarboxylase ◽  
Ethylene Biosynthesis ◽  
Gene Encoding ◽  
Ripening Process ◽  
Climacteric Fruit ◽  
Genes Encoding ◽  
Postharvest Ripening ◽  
Exogenous Ethylene

The banana, a typical climacteric fruit, undergoes a postharvest ripening process followed by a burst in ethylene production that signals the beginning of the climacteric period. Postharvest ripening plays an important role in improving the quality of the fruit as well as limiting its shelf life. To investigate the role of glutamate decarboxylase (GAD) in climacteric ethylene biosynthesis and fruit ripening in postharvest banana, a GAD gene was isolated from banana, designated MuGAD. Coincidently with climacteric ethylene production, MuGAD expression as well as the expression of the genes encoding the Musa 1-aminocyclopropane-1-carboxylate synthase (MaACS1) and Musa 1-aminocyclopropane-1-carboxylate oxidase (MaACO1) greatly increased during natural ripening and in ethylene-treated banana. Moreover, ethylene biosynthesis, ripening progress, and MuGAD, MaACS1, and MaACO1 expression were enhanced by exogenous ethylene application and inhibited by 1-methylcyclopropene (1-MCP). Taken together, our results suggested that MuGAD is involved in the fruit ripening process in postharvest banana.

scholarly journals Effect of 1-MCP on Apple Fruit Ripening and Volatile Production

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 536D-536 ◽  
Author(s):  
J. Song ◽  
M.S. Tian ◽  
D.R. Dilley ◽  
R.M. Beaudry
Keyword(s):  
Fruit Ripening ◽  
Ethylene Production ◽  
Quality Criterion ◽  
Apple Fruit ◽  
Climacteric Fruit ◽  
Aroma Production ◽  
Ethylene Action ◽  
Important Quality ◽  
The Relationship ◽  
Volatile Production

Aroma production by apple fruit is an important quality criterion and has been found to be a fruit-ripening-related process. 1-Methylcyclopropene (1-MCP), an effective ethylene action inhibitor, was used to study the relationship between volatile biosynthesis, ethylene action, and fruit ripening in `Golden Delicious' apple fruit. Pre-climacteric fruit were treated with 1-MCP vapors at a concentration of 500 parts per billion (v/v) at 23°C. 1-MCP prevented the climacteric rise of ethylene production, respiration, and volatile production, while untreated fruits developed typical climacteric changes in ethylene production, respiration and volatile production. Applying ethylene at 15–20 parts per million for 24 hr 11 days after 1-MCP treatment could not overcome the effect of 1-MCP, suggesting that 1-MCP inhibited ethylene action irreversibly. Interestingly, when 1-MCP-treated tissue were fed butanol and butyric acid, they converted these compounds to their corresponding esters butylacetate and butylbutanoate. Thus precursor supply is apparently limiting and appears to be ethylene-dependent.

scholarly journals 369 Relationship Between Ethylene Production and Accumulation of Cuticular Constituents in `Delicious' Apples During Fruit Ripening at Room Temperature

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 507C-507 ◽  
Author(s):  
Zhiguo Ju ◽  
William J. Bramlage
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Fruit Ripening ◽  
Ethylene Production ◽  
Room Temperature ◽  
Developmental Changes ◽  
Ethylene Synthesis ◽  
Fruit Peel ◽  
Carbohydrate Polymers ◽  
Fatty Acids And Alcohols

Developmental changes in total cuticle and cuticular constituents were studied with `Delicious' fruit. Total wax (0.31 mg/cm2) and total cutin (0.54 mg/cm2, including carbohydrate polymers) were low in young fruit. They increased during fruit growth and reached 1.41 and 2.47 mg/cm2 of fruit peel at harvest, respectively. During fruit ripening at 20 °C, total cutin did not change, but total wax increased rapidly and reached 2.15 mg•cm-2 at 6 weeks. The increase of cuticular wax paralleled the increase of internal ethylene in fruit. Wax was separated by column chromatograph into four portions, hydrocarbons and wax esters, free alcohols, free fatty acids, and diols. More than half of the diols was ursolic acid. During fruit development, more hydrocarbons and diols accumulated in cuticle than free fatty acids and alcohols. During fruit ripening, all of the four portions increased, coincident with the climacteric rise in ethylene, but the increase rates of free fatty acids and alcohols were higher than those of other portions. Aminoethoxyvinylglycine (AVG, 220 mg•L-1) preharvest treatment inhibited internal ethylene synthesis to below 0.5 μL•L-1 during 6 weeks at 20 °C, and also inhibited wax accumulation. Ethephon (200 mg/L) preharvest treatment increased ethylene production and accelerated wax accumulation. α-farnesene accumulation coincided with increased internal ethylene and paralleled free fatty acid and alcohol accumulation.

scholarly journals Brassinosteroids Suppress Ethylene Biosynthesis via Transcription Factor BZR1 in Pear and Apple Fruit

2020 ◽  
Author(s):  
Yinglin Ji ◽  
Yi Qu ◽  
Zhongyu Jiang ◽  
Xin Su ◽  
Pengtao Yue ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Ethylene Production ◽  
Plant Hormone ◽  
Expression Profiles ◽  
Acc Synthase ◽  
Ethylene Biosynthesis ◽  
Apple Fruit ◽  
Pear Fruit ◽  
Climacteric Fruit

ABSTRACTThe plant hormone ethylene is important for the ripening of climacteric fruit, such as pear (Pyrus ussuriensis), and the brassinosteroid (BR) class of phytohormones affects ethylene biosynthesis during ripening, although via an unknown molecular mechanism. Here, we observed that exogenous BR treatment suppressed ethylene production during pear fruit ripening, and that the expression of the transcription factor PuBZR1 was enhanced by epibrassinolide (EBR) treatment during pear fruit ripening. PuBZR1 was shown to interact with PuACO1, which converts 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, and suppress its activity. We also observed that BR-activated PuBZR1 bound to the promoters of PuACO1 and of PuACS1a, which encodes ACC synthase, and directly suppressed their transcription. Moreover, PuBZR1 suppressed the expression of transcription factor PuERF2 by binding its promoter, and PuERF2 bound to the promoters of PuACO1 and PuACS1a. We concluded that PuBZR1 indirectly suppresses the transcription of PuACO1 and PuACS1a through its regulation of PuERF2. Ethylene production and the expression profiles of the corresponding apple (Malus domestica) homologs showed similar changes following EBR treatment. Together, these results suggest that BR-activated BZR1 suppresses ACO1 activity and the expression of ACO1 and ACS1a, thereby reducing ethylene production during pear and apple fruit ripening. This likely represents a conserved mechanism by which exogenous BR suppresses ethylene biosynthesis during climacteric fruit ripening.One-sentence summaryBR-activated BZR1 suppresses ACO1 activity and expression of ACO1 and ACS1a, which encode two ethylene biosynthesis enzymes, thereby reducing ethylene production during pear and apple fruit ripening.

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