scholarly journals An Efficient Method to Evaluate Disease Resistance to Anthracnose by Tissue Culture of Strawberry Seedlings

2015 ◽  
Vol 14 (1) ◽  
pp. 1-6
Author(s):  
Keita Hirashima ◽  
Takao Katayama ◽  
Takaaki Ishii ◽  
Yasushi Shibato ◽  
Hisakazu Mitsui
Keyword(s):  
Tissue Culture ◽  
Disease Resistance ◽  
Efficient Method

Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses

2020 ◽  
Vol 142 (3) ◽  
pp. 493-504
Author(s):  
Shreeparna Ganguly ◽  
Gourab Ghosh ◽  
Sanatan Ghosh ◽  
Arnab Purohit ◽  
Rituparna Kundu Chaudhuri ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Efficient Method ◽  
Transformation System

A Comparison of Methods of Evaluating Resistance to Cylindrocladium crotalariae in Peanut Field Tests1

1983 ◽  
Vol 10 (2) ◽  
pp. 66-69 ◽  
Author(s):  
C. C. Green ◽  
M. K. Beute ◽  
J. C. Wynne
Keyword(s):  
Disease Resistance ◽  
Arachis Hypogaea ◽  
Efficient Method ◽  
Root Rot ◽  
Field Tests ◽  
Black Rot ◽  
Mean Square ◽  
Comparison Of Methods ◽  
Arachis Hypogaea L ◽  
The Mean

Abstract Three methods presently used to evaluate disease resistance in peanut (Arachis hypogaea L.) to Cylindrocladium black rot (CBR) were compared to identify the most efficient method to screen peanut lines in field tests. Forty randomly selected F2 families in the F5 generation from a cross of the CBR-resistant source NC 3033 with NC 6 and the two parents were evaluated for percent dead and diseased plants, root rot index and microsclerotia/g of root. Significant differences were detected between the parents (p = 0.05) and among segregates (familes) (p = 0.01) for percent dead and diseased plants only. Error components exceeded the mean square values for differences among segregates for the root rot index and microsclerotia/g of root resulting in no significant differences among entries for these traits and low to no correlations among traits. Percent dead and diseased plants was concluded to be the best of the three methods for screening peanut lines in the field for CBR resistance.

scholarly journals APPLICATION OF CALLUS CULTURE FOR THE STUDY OF BLACKSPOT DISEASE RESISTANCE IN ROSES

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1155e-1155
Author(s):  
J.M. Sherman ◽  
K.S. Reddy ◽  
S.E. Newman ◽  
J.A. Spencer
Keyword(s):  
Tissue Culture ◽  
Ascorbic Acid ◽  
Disease Resistance ◽  
Callus Formation ◽  
Callus Cultures ◽  
Protein Profiles ◽  
Biochemical Responses ◽  
Sds Page ◽  
Similarities And Differences ◽  
Basal Salts

The objective of this study was to determine whether tissue culture can be used for studying the blackspot resistance found in some roses. Callus was initiated from leaves, petioles, and stems of resistant and susceptible genotypes. Good callus formation for susceptible roses (hybrid teas) was obtained on a medium containing MS basal salts, vitamins, sucrose, and 8 g/L agar supplemented with 2 mg/L 2,4-D, 1 mg/L NAA, and 0.2 mg/L BA. Callus formation for resistant roses (species roses) was best when the concentrate ions of growth regulators in the medium were halved. Browning in species rose cultures, was decreased with the addition of 0.05 g/L ascorbic acid to the medium followed by incubation in the dark. The subculture calli were inoculated with the fungal conidia and were analyzed for proteins by SDS-PAGE. These protein profiles were compared to those of whole leaf samples. The results are discussed in terms of similarities and differences in the biochemical responses of callus cultures versus whole leaves to the blackspot infection.

scholarly journals In Vitro Selection and in Vitro Screening for Disease Resistance in Fruit Trees

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 695b-695
Author(s):  
Freddi A. Hammerschlag ◽  
Ghazala Hashmi ◽  
Robin Huettel ◽  
Dennis Werner ◽  
David Ritchie
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Disease Resistance ◽  
Pseudomonas Syringae ◽  
Xanthomonas Campestris ◽  
Fruit Trees ◽  
Culture Techniques ◽  
Somaclonal Variants ◽  
Tissue Culture Techniques

One approach for obtaining useful genetic variation is to select for somaclonal variants generated by tissue culture techniques. Increased levels of resistance to bacterial leaf spot (Xanthomonas campestris pv. pruni) have been observed in toxin-selected and unselected peach regenerants in vitro, in the greenhouse and under field conditions. Peach regenerants have also demonstrated increased levels of bacterial canker (Pseudomonas syringae pv. syringae) and root-knot nematode (Meloidogyne incognita) resistance. Random amplified polymorphic DNA (RAPD) primers have been used to study genetic variation at the DNA level among the somaclonal variants. Sixty RAPD primers (10-mers) were screened and 10 proved useful as markers to detect polymorphisms, thus establishing a genetic basis for somaclonal variation. These studies demonstrate the feasibility of using tissue culture techniques to generate fruit trees with increased levels of disease resistance.

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