scholarly journals Effects of Storage Temperature and Gibberellin Pretreatment on Germination Rate of Swertia japonica Seed

2011 ◽  
Vol 10 (3) ◽  
pp. 321-324 ◽  
Author(s):  
Mamiko Yamada ◽  
Satoru Tsukagoshi ◽  
Kazuma Yanagisawa ◽  
Maya Kaneko ◽  
Satoru Motoki ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Germination Rate ◽  
Swertia Japonica

scholarly journals Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay
Keyword(s):  
Relative Humidity ◽  
Storage Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Significant Decline ◽  
Rapid Decline ◽  
Seed Moisture Content ◽  
C Storage ◽  
Seed Moisture ◽  
Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

scholarly journals Effects of storage temperature and duration on pollen grain viability and pollen-tube elongation in Chinese chinqapin (Castanea henryi Skan)

2020 ◽  
Vol 49 (2) ◽  
pp. 297-304
Author(s):  
Huan Xiong ◽  
Deyi Yuan ◽  
Zhi-Yu Deng ◽  
Genhua Niu ◽  
Feng Zou
Keyword(s):  
Pollen Tube ◽  
Room Temperature ◽  
Storage Temperature ◽  
Southern China ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grain ◽  
Tube Length ◽  
Storage Duration ◽  
Pollen Tube Length

Chinese chinquapin [Castanea henryi (Skan) Rehder & E.H. Wilson] is used as a food and timber crop in southern China. Most chinquapin cultivars are self-incompatible and bloom at different times; consequently, artificial pollination is used to ensure fruit set and nut yield. Effective pollen storage that enables producers and breeders to use stored pollen for cross-pollination at a later date is important. In this study, the cultivar Changmangzi was used to estimate the viability and pollen tube length of pollen stored at room temperature, and at 4, −20, and −80°C using in vitro germination tests. It was observed that pollen grain germination significantly decreased at all four storage temperatures. Pollen viability was 14.4% after only 24 days of storage at room temperature. The germination rate was 13.3% after 90 days of storage at 4°C, and 14.5% after 180 days at −20°C. The initial germination rate of pollen stored at −80°C was 56.3% at the beginning of the test and decreased to 15.4% after 240 days. Pollen-tube length decreased with increased storage duration; mean pollen-tube lengths ranged from 109.44 to 257.51 μm. Based on these results, it is suggested that a storage temperature of −80°C for Changmangzi pollen is good.

scholarly journals Desiccation, dormancy, and storage of Pterocarya fraxinifolia (Juglandaceae) seeds: application in Hyrcanian and Colchian forest conservation

2020 ◽  
Vol 50 (1) ◽  
pp. 24-31
Author(s):  
Mikołaj Krzysztof Wawrzyniak ◽  
Anna Katarzyna Jasińska ◽  
Paweł Chmielarz ◽  
Gregor Kozlowski
Keyword(s):  
Moisture Content ◽  
Desiccation Tolerance ◽  
Storage Temperature ◽  
Germination Rate ◽  
Plant Genetic Resources ◽  
Natural Habitat ◽  
Seedling Emergence ◽  
Population Decline ◽  
Ex Situ ◽  
Seed Moisture Content

Pterocarya fraxinifolia (Poir.) Spach (Juglandaceae) is a model relict tree species native to South Caucasus and is a typical element of threatened riparian forests. Intensive land transformations, which are common in Transcaucasia, have resulted in loss of natural habitat and population decline of the species. One of the methods of ex situ conservation is seed banking. Cryopreservation in liquid nitrogen (−196 °C) is of particular interest, as it allows safe preservation of valuable plant genetic resources. However, the feasibility of seed cryopreservation is related to the desiccation tolerance and intrinsic composition of the seeds. In this study, we examined the physiological traits of Pterocarya fraxinifolia seeds, for which desiccation tolerance is unknown or controversial, and their feasibility for cryopreservation. Additionally, we tested stratification methods for dormancy assessment. Results showed that seeds survived desiccation to a moisture content of 2.8% with a germination rate of 64%. Stratification at a temperature of 3 °C for 8 weeks proved to be both fast and effective. Seed moisture content ranging from 2.8% to 18.1% was determined to be safe for cryopreservation. There was no difference in seedling emergence in seeds stored for 1 year regardless of the storage temperature (−3, −18, or −196 °C). Based on our results, Pterocarya fraxinifolia seeds can be classified as orthodox. This study demonstrates for the first time the feasibility of cryopreserving Pterocarya fraxinifolia seeds.

scholarly journals Respon Perkecambahan Polen Pepaya IPB 6 dan IPB 9 terhadap Penyimpanan pada Suhu Rendah

2015 ◽  
Vol 6 (1) ◽  
pp. 29
Author(s):  
Fidianinta , ◽  
Ketty Suketi ◽  
Winarso D. Widodo
Keyword(s):  
Low Temperature ◽  
Pollen Tube ◽  
Key Words ◽  
Reproductive Biology ◽  
Pollen Germination ◽  
Storage Temperature ◽  
Germination Rate ◽  
Experimental Results ◽  
Pollen Storage ◽  
Pollen Diameter

<p>ABSTRACT</p><p>An experiment was conducted to study the germination and storability of papaya pollen IPB 6 and  IPB  9  stored for 4 weeks at 3  levels  of  low temperature. The experiment was conducted at the Laboratory of Microtechnic and Laboratory of Biophysics  and  Reproductive Biology, Bogor Agricultural University in the month of April to June 2014. Three  variables  were observed in this experiment:  the germination rate, pollen diameter, and length of pollen. The experimental results showed that storage temperature affected the three variables observed. The best germination for IPB 6 was indicated by pollen storage at 10 0C (23.1%) while for IPB 9 was indicated by pollen stored at 5 0C (30.7%). The longest pollen tube  of  IPB 6  after storage  for 4 weeks  was indicated by pollen stored at -20 0C  (63 µm) while for IPB 9, indicated by pollen stored at 10 0C (47.72 µm). The experimental results showed that there was  no corellation between pollen diameter and length of pollen tube with pollen germination in papaya pollen of IPB 6 dan IPB 9.</p><p>Key words: diameter pollen, pollen tube, germination, storability</p><p> </p><p>ABSTRAK</p><p>Percobaan  dilakukan untuk  mempelajari daya simpan dan daya berkecambah  polen  pepaya IPB 6 dan IPB 9 yang disimpan pada suhu 5 0C, 10 0C, dan -20 0C dengan waktu penyimpanan 0-4 minggu.  Percobaan  dilaksanakan  di Laboratorium Mikroteknik dan Laboratorium Biofisik dan Biologi Reproduksi, Institut Pertanian Bogor pada bulan April-Juni 2014. Tiga variabel yang diamati pada percobaan ini adalah daya berkecambah, diameter polen dan panjang polen. Hasil percobaan menunjukkan  bahwa  suhu  penyimpanan mempengaruhi  ketiga  variabel yang  diamati. Daya berkecambah terbaik untuk pepaya IPB 6 ditunjukkan oleh polen yang disimpan pada 10 0C (23.1%)sedangkan  untuk  IPB 9 ditunjukkan oleh  polen  yang  disimpan pada 5 0C  (30.7%).  Tabung Polen pepaya  terpanjang  setelah  disimpan  selama  4 minggu  pada  IPB 6 ditunjukkan  oleh  polen  yang disimpan pada -20 0C (63 µm)  sedangkan untuk IPB 9 ditunjukkan oleh polen yang disimpan pada 10 0C (47.72 µm). Hasil  percobaan  menunjukkan  bahwa  tidak  ada  hubungan antara  diameter  dan panjang tabung polen dengan daya berkecambah pada polen pepaya IPB 9 dan IPB 6.</p><p>Kata kunci: daya berkecambah, daya simpan, diameter polen, tabung polen</p>

scholarly journals Effect of Storage Temperature on Biochemical and Mixolab Pasting Properties of Chinese Japonica Paddy

2014 ◽  
Vol 4 (2) ◽  
pp. 57
Author(s):  
Xing-jun Li ◽  
Ping Jiang
Keyword(s):  
Storage Temperature ◽  
Germination Rate ◽  
Soluble Sugars ◽  
Bromothymol Blue ◽  
Biochemical Properties ◽  
Pasting Properties ◽  
Water Soluble ◽  
Acid Dye ◽  
Scanning Calorimetry ◽  
Starch Gelatinization

<p>This study investigated the changes in germination rate, contents of oleic acids, protein, water soluble sugars, enthalpy of flour gelatinization, and Mixolab dough pasting properties of three varieties of <em>japonica</em> rough rice after 18-month storage at four temperatures of 4,15,25 and 35 ºC. After an 18-month storage, the paddy stored at or below 25 ºC had more than 70% germination rate, their flour extracts by acid dye mixture of methyl red and bromothymol blue showed greenish color, but the paddy stored at 35 ºC was only 30% germination rates, their flour extracts by acid dye mixture seem to be yellowish. With an increase in storage temperature, mositure content in paddy decreased, but total protein changed unsignificantly. In comparison to the paddy stored at 15 ºC, the higher storage temperature (25 and 35 ºC) tends to decrease the contents of damaged starch, water-soluble reducing sugars, total sugars, and uronic acids. The content of oleic acid in paddy stored at 4 ºC was markedly higher than that at the temperatures of 15, 25 and 35 ºC. Compared to lower temperate (4 and 15 ºC), the higher storage temperature (35 ºC) increased the gelatinization enthalpy of paddy flour and its starch determined by a differential scanning calorimetry (DSC), also increased Mixolab characteristic torque parameters such as starch gelatinization peak (C3), starch gelatinization minimum (C4), starch retrogradation minimum (C5), degrees of starch decay (C3-C4) and retrogradation (C5-C4) with a decrease in protein weakening (C2). The results of this study indicate storage temperature is an important factor affecting the physiological and biochemical properties of paddy, and lower temperature below room temperature are recommended to maintain paddy quality.</p>

scholarly journals Seed production characters and germination performance of reed canary grass in Finland

2000 ◽  
Vol 9 (3) ◽  
pp. 239-251 ◽  
Author(s):  
M. SAHRAMAA ◽  
L. HÖMMÖ
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Seed Yield ◽  
Seed Weight ◽  
Storage Temperature ◽  
Germination Rate ◽  
Reed Canary Grass ◽  
Harvest Time ◽  
Canary Grass ◽  
Thousand Seed Weight

Production of reed canary grass (Phalaris arundinacea L.) seed in Finland may be of interest in the near future. Seed production characters of cultivars Palaton and Venture were studied between 1995 and 1998 at Jokioinen. Effects of harvest time on seed yield, thousand seed weight and seed germination were evaluated for 'Palaton'. Seed yield varied from 2 to 432 kg ha-1, being only 100 kg ha-1 on average. In the years of highest seed yields the optimum harvest time was 15 days after complete anthesis. At that time high seed yield was associated with a low proportion of shattered seeds and high thousand seed weight and good seed germination. In an additional experiment, the effect of storage temperature and storage time on seed germination were investigated for 'Venture'. Storage temperature had no significant influence on final germination, but warmer storage significantly speeded up germination rate as recorded after 7 days of germination. After 7 days the percentage of germinated seeds was also significantly higher for seeds stored for >10 months than with seeds stored for

scholarly journals Determination of Optimal Storage Temperature to Maintain Viable Pawpaw [Asimina triloba (L.) Dunal] Seed

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 557A-557 ◽  
Author(s):  
Kirk W. Pomper ◽  
Snake C. Jones ◽  
LaTeasa Barnes
Keyword(s):  
Native American ◽  
Seed Germination ◽  
Storage Temperature ◽  
Germination Rate ◽  
Germplasm Collection ◽  
Peat Moss ◽  
Short Period ◽  
Seed Stratification ◽  
And Storage ◽  
Storage Temperatures

Pawpaw is a native American tree fruit that has great potential as a new commercial crop. The USDA National Clonal Germplasm Repository for Asimina sp. is located at Kentucky State Univ. (KSU); therefore, germplasm collection and storage are important components of the research program. Recalcitrant seeds do not tolerate desiccation, have a relatively short period of viability, and tend not to tolerate subfreezing temperatures. Since pawpaw seed shows a moderate level of recalcitrance, the objectives of this experiment were to determine which storage temperatures (20, 5, -15, and -70 °C for 8.5 weeks) would maintain viable seed, and whether prior seed stratification (5 °C for 17.5 weeks) would influence survival at the various storage temperatures. Seeds were placed in ziplock bags in moist peat moss and subjected to the range of storage temperatures either before or after stratification. After storage and stratification treatments, seed germination rate was examined for 10 weeks at 25 °C on moist filter paper in petri dishes. Both stratification and storage temperature significantly affected seed germination rate. Seeds did not germinate after storage at subfreezing temperatures, regardless of stratification treatment. The best germination rate, ≈70%, was obtained with stratification followed by storage at 20 °C. However, for long-term storage of viable nongerminating pawpaw seed, stratification followed by storage at 5 °C would be most appropriate. Subfreezing storage temperatures were found to be lethal to pawpaw seeds.

scholarly journals Germination Characteristics and Dynamic Changes of Antioxidant Enzymes during Storage of Viola dissecta Pollen

2021 ◽  
Vol 25 (04) ◽  
pp. 838-844
Author(s):  
Wenqing Jia
Keyword(s):  
Antioxidant Enzymes ◽  
Pollen Germination ◽  
Room Temperature ◽  
Storage Temperature ◽  
Germination Rate ◽  
Pollen Grains ◽  
Suitable Medium ◽  
Different Temperatures ◽  
Surface Ornamentation

Knowledge about pollen ultra-morphology, storage characteristics and germination rate are essential for directional plant breeding and plant improvement. The objective of this study was to determine a suitable medium for pollen germination in vitro of Viola dissecta and to evaluate the effect of different storage temperatures on its pollen longevity. The pollen ultra-morphology of V. dissecta was observed using scanning electron microscopy (SEM), the suitable medium for pollen germination in vitro was determined by orthogonal test. Dried pollen of V. dissecta was stored at different temperatures (room temperature, 4, -20 and -80°C) and different storage times (24, 40, 72, 120, 184, 264 and 365 d), the germination rate of the stored pollen and the activities of SOD, POD and CAT were investigated. Pollen grains of V. dissecta were medium-sized with three germination ditches. The surface ornamentation was smooth with small grains set on the surface, which was different from Viola spp. pollen. The most suitable medium for V. dissecta was composed of 285 g•L-1 sucrose, 6 g•L-1 agar, 50 mg•L-1 GA3, 250 mg•L-1 boric acid, and 200 mg•L-1 Ca(NO3)2, The best storage temperature of pollen was -80oC, after 365 d of storage, the germination rate was still 57.86%. During storage, the pollen germination rate decreased significantly after the peak of the activities of the three antioxidant enzymes. Correlation analysis showed that SOD was major factor affecting the germination rate of V. dissecta pollen, and it has a significant positive correlation with pollen germination rate, followed by CAT and POD. SOD was a sensitive antioxidant enzyme at room temperature, 4 and -80°C, whereas at -20°C, both SOD and CAT were sensitive antioxidant enzymes. © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers©

Optimising storage and in vitro germination of Eucalyptus pollen

2007 ◽  
Vol 55 (1) ◽  
pp. 83 ◽  
Author(s):  
Tasmien N. Horsley ◽  
Steven D. Johnson ◽  
Terrence K. Stanger
Keyword(s):  
Boric Acid ◽  
Pollen Germination ◽  
Storage Temperature ◽  
Sucrose Solution ◽  
Germination Rate ◽  
Pollen Viability ◽  
Eucalyptus Grandis ◽  
In Vitro Germination ◽  
Sucrose Solutions

The best sucrose solution for maximum in vitro germination of Eucalyptus pollen was investigated in order to evaluate pollen germination rate as an indicator of pollen viability. In vitro germination of both freshly collected and 1-year-old pollen (stored at 4°C) of Eucalyptus grandis, E. smithii, E. nitens, E. dunnii and E. macarthurii was carried out in 0, 10, 20, 30, 40 and 50% (w/v) sucrose solutions, either with (0.15 mg L–1) or without boric acid. Similar trends were obtained for both fresh and 1-year-old pollen, with all species responding most favourably to 30% (w/v) sucrose and 0.15 mg L–1 boric acid. When an optimal in vitro germination medium had been established, the viabilities (%germination) of E. smithii, E. nitens and E. grandis pollen, stored at room (25°C), fridge (4°C), freezer (–10°C) and liquid nitrogen (–196°C) temperatures, were compared. For all tested species, germination declined as storage temperature increased, and by 8 months, the highest survival was obtained with cryostored pollen.

scholarly journals Temporary storage of jussara palm seeds: effects of time, temperature and pulp on germination and vigor

2004 ◽  
Vol 22 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Cibele C. Martins ◽  
Marilene L. A. Bovi ◽  
João Nakagawa ◽  
Gentil Godoy Júnior
Keyword(s):  
Storage Temperature ◽  
Germination Rate ◽  
High Sensitivity ◽  
Sao Paulo ◽  
São Paulo ◽  
Test Installation ◽  
Paulo State ◽  
Euterpe Edulis ◽  
São Paulo State ◽  
Fruit Harvest

Seeds of jussara palm (Euterpe edulis Mart.) are recalcitrant, presenting low longevity, high sensitivity to dehydration and to low temperatures of storage. Temperature conditions for temporary preservation of those seeds, with and without pulp, were studied. Mature fruits were harvested from 24 plants belonging to the palm collection of the Instituto Agronômico (IAC) located at Ubatuba, São Paulo State, Brazil, and taken in moisture-proof containers to the Universidade Estadual de São Paulo, in Botucatu, São Paulo State. Half of the fruits were shelled and the seeds stored, with and without pulp, in sealed plastic bags (20 µm thick) maintained in chambers with temperatures of 5; 10; 15 and 20-30ºC. Samples for the quality tests were taken at 0; 3; 6; 9 and 12 days after fruit harvest. Seeds stored with pulp were immediately shelled before test installation. Several characteristics were evaluated, especially seed water content, germination rate, seedling length and dry matter. There is a positive after-ripening effect on Euterpe edulis seeds. A period of storage of 9 to 12 days, after fruit harvest and before sowing, favored germination and vigor of jussara seeds. These effects were higher in shelled seeds when compared to unshelled ones. Short-term storage temperatures ranging from 5 to 20-30ºC are equally adequate for the preservation of seeds without pulp, but should not exceed 20ºC for those with pulp, since a decrease in germination and vigor, and an increase in rotted germinative button and dead seeds were observed at 20-30ºC temperature.

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