scholarly journals Characteristics in Polyploid Meiwa Kumquat (Fortunella crassifolia Swingle) Induced by Colchicine Treatment to Nucellar Embryos and Their Utilization for Triploid Breeding

2011 ◽  
Vol 10 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Tsunaki Nukaya ◽  
Tomohiro Ohta ◽  
Kiichi Yasuda ◽  
Masaki Yahata ◽  
Hisato Kunitake ◽  
...  
Keyword(s):  
Colchicine Treatment ◽  
Triploid Breeding ◽  
Nucellar Embryos

Characteristics in autotetraploid kumquats (Fortunella spp.) induced by colchicine treatment to nucellar embryos and their utilization for triploid breeding

2019 ◽  
Vol 245 ◽  
pp. 210-217 ◽  
Author(s):  
Tsunaki Nukaya ◽  
Miki Sudo ◽  
Masaki Yahata ◽  
Yoshiyuki Nakajo ◽  
Tomohiro Ohta ◽  
...  
Keyword(s):  
Colchicine Treatment ◽  
Triploid Breeding ◽  
Nucellar Embryos

scholarly journals The Confirmation of a Ploidy Periclinal Chimera of the Meiwa Kumquat (Fortunella crassifolia Swingle) Induced by Colchicine Treatment to Nucellar Embryos and Its Morphological Characteristics

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 562 ◽  
Author(s):  
Tsunaki Nukaya ◽  
Miki Sudo ◽  
Masaki Yahata ◽  
Tomohiro Ohta ◽  
Akiyoshi Tominaga ◽  
...  
Keyword(s):  
Ploidy Level ◽  
Sugar Content ◽  
Flow Cytometric Analysis ◽  
Morphological Characteristics ◽  
Colchicine Treatment ◽  
Reproductive Organs ◽  
Periclinal Chimera ◽  
High Sugar Content ◽  
Diploid Cells ◽  
Nucellar Embryos

A ploidy chimera of the Meiwa kumquat (Fortunella crassifolia Swingle), which had been induced by treating the nucellar embryos with colchicine, and had diploid (2n = 2x = 18) and tetraploid (2n = 4x = 36) cells, was examined for its ploidy level, morphological characteristics, and sizes of its cells in its leaves, flowers, and fruits to reveal the ploidy level of each histogenic layer. Furthermore, the chimera was crossed with the diploid kumquat to evaluate the ploidy level of its reproductive organs. The morphological characteristics and the sizes of the cells in the leaves, flowers, and fruits of the chimera were similar to those of the tetraploid Meiwa kumquat and the ploidy periclinal chimera known as “Yubeni,” with diploids in the histogenic layer I (L1) and tetraploids in the histogenic layer II (L2) and III (L3). However, the epidermis derived from the L1 of the chimera showed the same result as the diploid Meiwa kumquat in all organs and cells. The sexual organs derived from the L2 of the chimera were significantly larger than those of the diploid. Moreover, the ploidy level of the seedlings obtained from the chimera was mostly tetraploid. In the midrib derived from the L3, the chimera displayed the fluorescence intensity of a tetraploid by flow cytometric analysis and had the same size of the cells as the tetraploid and the Yubeni. According to these results, the chimera is thought to be a ploidy periclinal chimera with diploid cells in the outermost layer (L1) and tetraploid cells in the inner layers (L2 and L3) of the shoot apical meristem. The chimera had desirable fruit traits for a kumquat such as a thick pericarp, a high sugar content, and a small number of developed seeds. Furthermore, triploid progenies were obtained from reciprocal crosses between the chimera and diploid kumquat.

scholarly journals Megaspore Chromosome Doubling in Eucalyptus urophylla S.T. Blake Induced by Colchicine Treatment to Produce Triploids

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 728 ◽  
Author(s):  
Jun Yang ◽  
Jianzhong Wang ◽  
Zhao Liu ◽  
Tao Xiong ◽  
Jun Lan ◽  
...  
Keyword(s):  
Tree Species ◽  
Chromosome Doubling ◽  
Treatment Plant ◽  
Solution Treatment ◽  
Colchicine Treatment ◽  
Treatment Technique ◽  
Chromosome Counting ◽  
Triploid Breeding ◽  
Optimal Period ◽  
Colchicine Solution

Triploids generally provide an advantage in vegetative growth in forest trees. However, the technique of triploid breeding is still an open field in the Eucalyptus tree species. This study aims to explore the colchicine treatment technique for megaspore chromosome doubling to establish triploids in this tree species. Cytological observation on microsporogenesis and megasporogenesis was carried out to guide megaspore chromosome doubling induced by colchicine treatment. Ploidy level in progenies was detected by flow cytometry and somatic chromosome counting. A relationship between microsporogenesis and megasporogenesis was established to guide the colchicine treatment. Seven triploids were obtained in the progenies, and the highest efficiency of triploid production was 6.25% when the flower buds underwent a 0.25% colchicine solution treatment for 6 h using an aspiration method seven days after the first observation of leptotene during microsporogenesis on the floral branch. Cytological analysis showed that the megasporocyte from leptotene to diakinesis may be the optimal period for megaspore chromosome doubling by colchicine treatment. Plant height, ground diameter, leaf area, and the photosynthetic parameter of triploid eucalypt were significantly higher than those of the diploid plant at 6 months old. Hybridization with 2n megaspores induced by colchicine treatment is an effective way for Eucalyptus triploid breeding. These results should accelerate the development of advanced germplasms in this tree species.

Physiological and anatomical changes in Thai rice landrance (Oryza sativa L.) cv Pakaumpuel after colchicine treatment

2020 ◽  
Vol 52 (5) ◽  
Author(s):  
Worasitikulya Taratima ◽  
Pradub Reanprayoon ◽  
Sayam Raso ◽  
Mallika Chantarangsee ◽  
Pitakpong Maneerattanarungroj
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Colchicine Treatment ◽  
Anatomical Changes

Methods of experimental polyploidization and their use in apple breeding

2020 ◽  
Vol 62 ◽  
pp. 85-90
Author(s):  
L. V. Tashmatova ◽  
O. V. Matsneva ◽  
T. M. Khromova ◽  
V. V. Shakhov
Keyword(s):  
Exposure Time ◽  
Cell Walls ◽  
Prolonged Exposure ◽  
Ornamental Plants ◽  
Colchicine Treatment ◽  
Apple Trees ◽  
Open Ground ◽  
High Concentrations ◽  
Diploid Gametes

The article presents methods of experimental polyploidy of fruit, berry and ornamental plants. The purpose of this review is to highlight the problems and prospects of polyploidization of plants in the open ground and in vitro culture and the possibility of their application for apple trees. For the purpose of obtaining apple tetraploids as donors of diploid gametes, seed seedlings were treated with a solution of colchicine in concentrations of 0.1-0.4 % for 24 and 48 hours. Colchicine concentrations of 0.3 % and 0.4 % at 48 hours of treatment had a detrimental eff ect on their development. As a result, tetraploids and chimeras were obtained from seeds from free pollination of the varieties Orlik, Svezhest, Kandil Orlovsky, as well as from seeds obtained from crossing the varieties Svezhest×Bolotovskoe, Moskovskoe Оzherel’e×Imrus, Girlyanda×Venyaminovskoe. The optimal concentration of colchicine was 0.1 %. Methods of colchicine treatment have been studied: 1) adding to the nutrient medium, colchicine concentration: 0.01%, 0.02%, exposure time 24h-19 days; 2) applying amitotic solution to the growth point, colchicine concentration: 0.1 %, 0.2 %, exposure time 24h-7 days. To increase the penetration of colchicine through the cell walls, a 0.1 % dimexide solution was used. Studies have shown that high concentrations and prolonged exposure to colchicine reduce the viability of explants.

scholarly journals Hybrid Triploid Induced by Megaspore Chromosome Doubling in Jujube (Ziziphus jujuba Mill.) ‘Maya’ and Its Characteristics

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 112
Author(s):  
Songshan Liu ◽  
Chenxing Zhang ◽  
Weicong Yang ◽  
Xiang Li ◽  
Lu Hou ◽  
...  
Keyword(s):  
Chromosome Doubling ◽  
Colchicine Treatment ◽  
Fruit Trees ◽  
Growth Stress ◽  
Optimal Timing ◽  
Photosynthetic Parameters ◽  
Flower Bud ◽  
Treatment Methods ◽  
Triploid Individual ◽  
Colchicine Solution

Polyploid breeding is an important strategy for tree improvement because polyploid individuals typically show superior traits, such as improved growth, stress resistance, and superior fruit quality. Artificial induction of chromosome doubling of female gametes is an effective approach to obtain triploid progeny. However, no triploid fruit tree cultivars have been developed using this approach. The objective of this study was to explore the utility of chromosome doubling in female gametes of ‘Maya’ jujube to produce triploid individuals. The temporal relationship between flower bud morphology and the megaspore meiotic stage was studied to guide the optimal timing of colchicine treatment. Colchicine solution was applied to bearing shoots of mature ‘Maya’ jujube trees in a field experiment using two treatment methods (improved cotton leaching and injection method) and three concentrations (0.3%, 0.4%, and 0.5%). The water transport rate of ‘Maya’ jujube shoots was studied using dye solution to judge the effectiveness and timing of the colchicine treatment methods. Two triploids were identified among the progenies from the colchicine-treated shoots. The highest efficiency of triploid production was 3.3% when flower buds of diameter 1.76–2.12 mm were treated with 0.3% colchicine solution for 4 h using an improved cotton leaching method. The ground diameter, plant thorn length, leaf width, leaf area, stomatal length, stomatal width, chlorophyll content, and photosynthetic parameters of one triploid individual were significantly higher than those of diploids of identical parentage at 18 months old. Thus, induction of 2n megaspores is an effective approach to generate triploid jujube. These results are expected to promote and accelerate triploid breeding in fruit trees.

scholarly journals Colchicine treatment can improve outcomes of coronavirus disease 2019 (COVID‐19): A systematic review and meta‐analysis

2021 ◽  
Author(s):  
Timotius Ivan Hariyanto ◽  
Devina Adella Halim ◽  
Claudia Jodhinata ◽  
Theo Audi Yanto ◽  
Andree Kurniawan
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Colchicine Treatment

Effect of the Pairing Gene Ph1 and Premeiotic Colchicine Treatment on Intra- and Interchromosome Pairing of Isochromosomes in Common Wheat

Genetics ◽  
1998 ◽  
Vol 150 (3) ◽  
pp. 1199-1208 ◽  
Author(s):  
Juan M Vega ◽  
Moshe Feldman
Keyword(s):  
Common Wheat ◽  
Homologous Chromosome ◽  
Colchicine Treatment ◽  
Crossing Over ◽  
Meiotic Pairing ◽  
Factors Affecting ◽  
Homologous Chromosomes ◽  
Polyploid Wheat ◽  
Main Gene ◽  
Ph1 Gene

Abstract The analysis of the pattern of isochromosome pairing allows one to distinguish factors affecting presynaptic alignment of homologous chromosomes from those affecting synapsis and crossing-over. Because the two homologous arms in an isochromosome are invariably associated by a common centromere, the suppression of pairing between these arms (intrachromosome pairing) would indicate that synaptic or postsynaptic events were impaired. In contrast, the suppression of pairing between an isochromosome and its homologous chromosome (interchromosome pairing), without affecting intrachromosome pairing, would suggest that homologous presynaptic alignment was impaired. We used such an isochromosome system to determine which of the processes associated with chromosome pairing was affected by the Ph1 gene of common wheat—the main gene that restricts pairing to homologues. Ph1 reduced the frequency of interchromosome pairing without affecting intrachromosome pairing. In contrast, intrachromosome pairing was strongly reduced in the absence of the synaptic gene Syn-B1. Premeiotic colchicine treatment, which drastically decreased pairing of conventional chromosomes, reduced interchromosome but not intrachromosome pairing. The results support the hypothesis that premeiotic alignment is a necessary stage for the regularity of meiotic pairing and that Ph1 relaxes this alignment. We suggest that Ph1 acts on premeiotic alignment of homologues and homeologues as a means of ensuring diploid-like meiotic behavior in polyploid wheat.

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