scholarly journals Heat Shock Protein 70 and Anti-heat Shock Protein 70 Antibodies in Nasal Secretions of Patients with Chronic Rhinosinusitis

2016 ◽  
Vol 7 (1) ◽  
pp. ar.2016.7.0149 ◽  
Author(s):  
Namjil N. Tsybikov ◽  
Elena V. Egorova ◽  
Boris I. Kuznik ◽  
Elena V. Fefelova ◽  
Eli Magen
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Chronic Rhinosinusitis ◽  
Heat Shock Protein 70 ◽  
Nasal Polyps ◽  
Immunoglobulin E ◽  
Clinical Severity ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Levels

Background The issue of heat shock protein (HSP) 70 and anti-HSP70 antibodies in chronic rhinosinusitis (CRS) has never been explored. Objective To determine the nasal secretion (NS) levels of HSP70 and anti-HSP70 antibodies in patients with CRS with nasal polyps (CRSwNP) and patients with CRS without nasal polyps (CRSsNP), and to evaluate their associations with CRS clinical severity and correlation with NS interleukin (IL), IL-5 and interferon λ. Methods CRS severity was determined by Lund-Mackay scores. Levels of immunoglobulin E (IgE), IL-4, IL-5, interferon A, HSP70, and anti-HSP70 antibody levels in NS were measured by enzyme-linked immunosorbent assay. Results Forty-six patients with CRSsNP (25 women [543%] and 21 men [45.7%], mean [standard deviation {SD}]) age, 34.1 ± 123 years; 54 patients with CRSwNP (24 women [44.4%] and 30 men [55.6%], mean [SD] age, 37.9 ± 17.5 years). A group of 40 healthy subjects served as controls. Compared with the controls (with a mean [SD] NS HSP70 level of 0.05 ± 0.03 μg/mL), mean [SD]NS HSP70 levels in both the CRSsNP group (0.16 ± 0.07 ixg/mL) and CRSwNP group (0.21 ± 0.10 μg/mL) were increased (p < 0.001). Similarly, the mean (SD) NS anti-HSP70 antibody levels were significantly higher in patients with CRSwNP (0.25 ± 0.09 optical density value [ODV]) compared with CRSsNP (0.13 ± 0.04 ODV) (p < 0.001) and healthy controls (0.14 ± 0.02 ODV) (p < 0.001). NS HSP70 in subjects with CRSwNP showed a significant positive correlation with the Lund-Mackay score (r = 0.31; p < 0.05). NS levels of either HSP70 or anti-HSP70 antibodies were strongly correlated with NS IL-4 in the CRSwNP group (r = 0.62, p < 0.001; and r = 0.69, p < 0.001, respectively). Conclusion NS concentrations of HSP70 and secretory IgA anti HSP70 antibodies are increased in CRSwNP (but not in CRSsNP) and correlate positively with the Lund-Mackay score, NS IL-4, and NS IL-5.

Relationship between heat shock protein 70 and B amyloids in patients with alzheimer disease and vascular dementia

2011 ◽  
Vol 26 (S2) ◽  
pp. 505-505
Author(s):  
Y. Fu ◽  
S. Xiao
Keyword(s):  
Heat Shock ◽  
Alzheimer Disease ◽  
Heat Shock Protein ◽  
Vascular Dementia ◽  
Heat Shock Protein 70 ◽  
High Plasma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Statistical Manual ◽  
The Relationship ◽  
Normal Controls

AimsExplore the relationship between levels of plasma Aβ1–40, Aβ1–42 and heat shock protein 70 (HSP70) in patients with Alzheimer disease (AD) and vascular dementia (VD) and in elderly non-demented controls.Methods23 patients with AD and 21 patients with VD who meet diagnostic criteria of the Diagnostic Statistical Manual 4th edition and 20 control subjects were enrolled, administered the Mini-Mental State Exam (MMSE) and the Activity of Daily Living (ADL) inventory and their levels of plasma Aβ1–40, Aβ1–42 and HSP70 were measured by sandwich enzyme-linked immunosorbent assay.ResultsThe levels of plasma Aβ1–40, Aβ1–42 and the Aβ1–40/Aβ1–42 ratio were not significantly different across groups, but levels of plasma HSP70 in VD patients was significantly higher than in AD patients and in normal controls (3.19 vs 1.91 vs 1.43ng/ml, respectively; F=6.464, P=0.003). In the AD group MMSE scores were inversely correlated with ADL scores (r=-0.617, P=0.002) and with levels of plasma HSP70 (r=-0.437, P=0.037); but HSP70 levels were positively correlated with age (r=0.616, P=0.002) and with plasma Aβ1–40 (r=0.497, P=0.016) in AD group. In the VD group levels of plasma HSP70 were positively correlated with plasma Aβ1–40 (r=0.436, P=0.048).ConclusionsOur findings provide further evidence that high plasma HSP70 levels may play a role in the diagnosis and differential diagnosis of AD. HSP70 levels in AD patients is inversely associated with cognitive performance and positively correlated with plasma Aβ1–40. Plasma HSP70 in VD patients is significantly elevated and positively correlated with plasma Aβ1–40.

Cognitive Function and Heat Shock Protein 70 in Children With Temporal Lobe Epilepsy

2016 ◽  
Vol 32 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Azza M. Oraby ◽  
Ehab R. Abdol Raouf ◽  
Mostafa M. El-Saied ◽  
Maha K. Abou-Khadra ◽  
Suzette I. Helal ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Temporal Lobe Epilepsy ◽  
Temporal Lobe ◽  
Heat Shock Protein 70 ◽  
Short Term Memory ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Composite Scores

We conducted the present study to examine cognitive function and serum heat shock protein 70 levels among children with temporal lobe epilepsy. The Stanford-Binet Intelligence Test was carried out to examine cognitive function in 30 children with temporal lobe epilepsy and 30 controls. Serum heat shock protein 70 levels were determined with an enzyme-linked immunosorbent assay. The epilepsy group had significantly lower cognitive function testing scores and significantly higher serum heat shock protein 70 levels than the control group; there were significant negative correlations between serum heat shock protein 70 levels and short-term memory and composite scores. Children with uncontrolled seizures had significantly lower verbal reasoning scores and significantly higher serum heat shock protein 70 levels than children with controlled seizures. Children with temporal lobe epilepsy have cognitive dysfunction and elevated levels of serum heat shock protein 70, which may be considered a stress biomarker.

scholarly journals Immunopathology Features of Chronic Rhinosinusitis in High-altitude Dwelling Tibetans

2013 ◽  
Vol 4 (2) ◽  
pp. ar.2013.4.0055 ◽  
Author(s):  
Ba Luo ◽  
Liu Feng ◽  
Du Jintao ◽  
Liu Yafeng ◽  
Liu Shixi ◽  
...  
Keyword(s):  
Mast Cells ◽  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Transforming Growth Factor ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Inflammatory Cells ◽  
Cold Weather ◽  
Enzyme Linked Immunosorbent Assay ◽  
Treg Function

Chronic rhinosinusitis (CRS) presents distinct inflammatory and remodeling patterns in different populations and environments. Tibetan ethnic groups live at high altitudes and in cold weather conditions. We sought to examine whether Tibetans exhibit distinct CRS pathology or characteristics. Sinonasal polyps and mucosal tissue were obtained from 14 Tibetan patients with CRS and nasal polyps (CRSwNPs), 13 patients with CRS without nasal polyps (CRSsNPs), and 12 Tibetan controls. Tissue homogenates and serum samples were assayed for several T-helper (TH) cell cytokines and mediators using enzyme linked immunosorbent assay profiles were measured using quantity polymerase chain reaction. Several key inflammatory cells were examined for immunohistochemical markers. CRSwNPs were characterized by increased mediator promoting eosinophilic inflammation (interleukin [IL]-5, eosinophil cationic protein, and total immunoglobulin E) and slight synergism with expression of IL-8, IL-2sRa, IL-1beta, IL-6, and myeloperoxidase, and a predominance of eosinophils, mast cells, and neutrophils. GATA-3 transcription factor was significantly increased and Foxp3 showed a tendency to be impaired in CRSwNPs compared with controls. CRSsNPs were characterized by significantly high levels of transforming growth factor beta1, increased interferon γ, and a significant enhancement of Foxp3 and T-beta compared with CRSwNPs. There were reduced numbers of inflammatory cells but increased levels of macrophages in CRSsNPs. Compared with CRSsNPs, CRSwNPs present a severe inflammatory reaction and show a TH2 milieu with apparently impaired regulatory T cells (Treg) function and increased inflammatory cells infiltration predominated by eosinophilic and mast cells. In contrast, TH1 polarization with enhanced Treg function and increased levels of macrophages appear in CRSsNPs.

scholarly journals Oyster heat shock protein 70 plays a role in binding of human noroviruses

2021 ◽  
Author(s):  
Zilei Zhang ◽  
Danlei Liu ◽  
Qingping Wu ◽  
Dapeng Wang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Specific Binding ◽  
Surface Display ◽  
Cell Surface Display ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oyster Tissue ◽  
Human Noroviruses ◽  
Viral Particles

Human noroviruses (HuNoVs) are important foodborne pathogens causing acute gastroenteritis. Oysters are an important vehicle for transmission of HuNoVs. Histo-blood group antigen (HBGA)-like substances are considered the primary ligands for bioaccumulation of HuNoVs in oyster tissues. In this study, proteinaceous ligands for specific binding of HuNoVs were mined from oyster tissues using a bacterial cell surface display system. The macromolecular target was captured and identified in proteomic analysis. The distribution of viral particles, oyster heat shock protein 70 (oHSP 70), and type A HBGA (positive control) in oyster tissue was investigated by multiplex immunofluorescence assays after artificial contamination with HuNoVs (GII.4). Our results demonstrated that oHSP 70 is a candidate vital ligand for specific binding of HuNoVs in oyster tissues. In addition, P proteins (GI.1 and GII.4) and viral particles (GI.1 and GII.4) were captured by recombinant oHSP 70 in an enzyme-linked immunosorbent assay with sample signal/negative signal of 7.8, 6.3, 17.0, and 8.8, respectively. The findings suggested that oHSP 70 plays an important role in the binding of these foodborne viruses. Importance Human noroviruses (HuNoVs) are the most important pathogen for non-bacterial epidemic gastroenteritis cases. Foodborne transmission plays an important role in HuNoVs infection. Oysters, filter-feeding epibenthic bivalves, can be contaminated by faecal discharge in harvest water. A new proteinaceous ligand for HuNoVs other than HBGA is identified in oyster tissues. The significance of our research is in identifying and verifying the ligands in oyster tissues for HuNoVs binding. Our data will allow a better understanding of HuNoVs attachment and transmission with oysters, leading to control of undesired foodborne disease.

scholarly journals Serum heat shock protein 70 levels as a biomarker for inflammatory processes in multiple sclerosis

2018 ◽  
Vol 4 (2) ◽  
pp. 205521731876719 ◽  
Author(s):  
Patricia Lechner ◽  
Dorothea Buck ◽  
Lisa Sick ◽  
Bernhard Hemmer ◽  
Gabriele Multhoff
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Neurological Diseases ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Potential Biomarker ◽  
Inflammatory Processes

Background Inflammatory and neurodegenerative processes are hallmarks of multiple sclerosis (MS). The synthesis of the major stress-inducible heat shock protein 70 (Hsp70) is induced by inflammation. Objective The purpose of this study is to determine whether Hsp70 in serum can serve as a potential biomarker to distinguish inflammatory and neurodegenerative processes in MS. Methods Serum was obtained from 94 patients: 26 clinically isolated syndrome (CIS), 40 relapsing–remitting MS (RRMS), 19 secondary progressive MS (SPMS), and nine primary progressive MS (PPMS). As controls, serum samples were collected from patients with non-inflammatory neurological diseases (NINDs, n = 41), other inflammatory neurological diseases (OINDs, n = 28) and healthy donors (HDs, n = 114). Serum levels of Hsp70 were quantified using the enzyme-linked immunosorbent assay detecting free and liposomal Hsp70 (lipHsp70 ELISA). Results Patients with MS displayed significantly higher Hsp70 serum levels than HDs ( p < 0.001) and significantly lower levels than OINDs ( p = 0.001). A subgroup analysis revealed that Hsp70 serum levels of CIS/RRMS patients are significantly higher than those of patients with progressive MS (SPMS/PPMS) ( p < 0.05). Conclusion Inflammation causes the release of Hsp70 into the blood. As CIS/RRMS are associated with higher Hsp70 serum levels than progressive MS, serum Hsp70 levels might provide a marker for inflammatory processes.

Effects of pepsin A on heat shock protein 70 response in laryngopharyngeal reflux patients with chronic rhinosinusitis

2017 ◽  
Vol 137 (12) ◽  
pp. 1253-1259 ◽  
Author(s):  
Jing Wang ◽  
Zhao Yu ◽  
Jianjun Ren ◽  
Yang Xu ◽  
Yuke Zhang ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Chronic Rhinosinusitis ◽  
Heat Shock Protein 70 ◽  
Laryngopharyngeal Reflux

scholarly journals Anti-Heat Shock Protein 70 antibody levels are increased in myasthenia gravis and Guillain-Barré syndrome

2010 ◽  
Vol 225 (1-2) ◽  
pp. 180-183 ◽  
Author(s):  
Geir Helgeland ◽  
Axel Petzold ◽  
Jana Midelfart Hoff ◽  
Nils Erik Gilhus ◽  
Gordon T. Plant ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Myasthenia Gravis ◽  
Heat Shock Protein 70 ◽  
Guillain Barre Syndrome ◽  
Guillain Barré Syndrome ◽  
Guillain Barré ◽  
Antibody Levels

An enzyme‐linked immunosorbent assay for heat‐shock protein 70 in plants

1991 ◽  
Vol 3 (1) ◽  
pp. 29-36 ◽  
Author(s):  
K. G. Adham ◽  
M. C. Wilkinson ◽  
C. J. Smith ◽  
D. L. Laidman
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Immunosorbent Assay ◽  
Heat Shock Protein 70 ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Pengaruh heat shock protein-70 (HSP70) dan heat shock factor-1 (HSF-1) pada polip hidung

2016 ◽  
Vol 46 (1) ◽  
pp. 16
Author(s):  
Iwan Setiawan Adji ◽  
Juliati Hood Assegaf ◽  
Manshur Shidiq Wiyadi
Keyword(s):  
Wound Healing ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Nasal Polyps ◽  
Heat Shock Factor ◽  
Hsp70 Expression ◽  
Heat Shock Factor 1 ◽  
Impaired Wound Healing ◽  
Unsatisfactory Result

Latar belakang: Penyebab rekurensi polip hidung multifaktorial dan pertumbuhan polip didugakarena pemulihan luka yang terganggu, dengan HSP70 dan HSF-1 sebagai molekul yang berperan.Tujuan: Mengetahui pengaruh HSP70 dan HSF-1 pada kejadian tumbuh polip hidung.Metode: Penelitiandi Rumah Sakit Umum Daerah Karanganyar, dengan desain potong lintang dan pengambilan percontohsecara berurutan untuk mengamati kejadian tumbuh polip hidung, menggunakan polip yang tumbuhkembali pasca terapi polipektomi sederhana dan kortikosteroid topikal selama 6 minggu. Pewarnaanimunohistokimia dilakukan untuk menilai imunoreaktivitas secara kuantitatif pada HSP70 dan HSF-1area epitel, area stroma dan area total (area epitel dan area stroma).Hasil: Ekspresi rerata HSP70 total(stroma dan epitel), stroma saja dan epitel saja lebih rendah bermakna (p<0,05) pada polip yang tumbuhkembali. Peningkatan ekspresi HSF-1 epitel meningkat bermakna pada polip yang tumbuh kembali(p=0,041<0,05), sedangkan ekspresi HSF-1 total dan area stroma mengalami peningkatan tetapi secarastatistik tidak bermakna (P>0,05). Hasil uji korelasi antara perubahan ekspresi HSP70 dan perubahanekspresi HSF-1 pada tiga area adalah bermakna (p<0,05). Nilai korelasi Pearson sebesar -0,540 (area total),-0,521 (area stroma) dan -0,517 (area epitel), menunjukkan korelasi negatif dengan kekuatan korelasi yangsedang.Kesimpulan: Penurunan ekspresi HSP70 total, stroma dan epitel, berpengaruh pada kejadianpolip hidung yang tumbuh kembali, sedangkan peningkatan HSF-1 hanya pada epitel yang berpengaruhpada kejadian polip hidung tumbuh. Didapati korelasi berkekuatan sedang dengan arah negatif, yaitupeningkatan ekspresi HSF-1 sementara ekspresi HSP70 menurun. Kata kunci: Heat shock protein 70, heat shock factor-1, polip hidung ABSTRACTBackground: Pathogenesis of chronic rhinosinusitis with nasal polyps is multifactorial, andremained unclear with unsatisfactory result of its treatment. Evidence suggests growth of polypsallegedly due to impaired wound healing. HSP 70 and HSF-1 have role in wound healing but its effecton the growth of nasal polyps is unknown. Objective: To determine the effect of HSP70 and HSF-1 innasal polyp growth. Methods: The study was performed in Distric Hospital of Karanganyar, involvingpatients who were consecutively sampled, to observe re-growth of the polyps after simple polypectomy andtopical corticosteroids therapy for 6 weeks. Immunoreactive cells of HSP70 and HSF-1 were calculatedin epithelium area, stroma area and total area of epithelium and stroma. Results: A decrease expressionof HSP70 in the total area (stroma and epithelium), stroma and epithelium were found in re-growth NPtissue, and statistically significant (p <0.05). An increased expression of epithelial HSF-1 was statisticallysignificant (p=0.041<0.05), while of total and stromal HSF-1 was statistically not significant (p>0.05).A decrease of the HSP70 and an enhanced of HSF-1 expression was significant (p <0.05). Pearsoncorrelation value of -0.540 for total area, -0.521 for stroma area and -0.517 for epithelium area, showed a negative correlation with moderate strength of the correlation. Conclusion: A decreased expressionof total, epithelial and stromal area of HSP70 influence the incidence of NP re-growth. Only epithelialHSF-1 associated with regrew of NP. The moderate strength of correlation with a negative directionoccurs in HSF-1 and HSP70 expression. Keywords: Heat shock protein 70, heat shock factor-1, nasal polyps Alamat korespondensi: DR. Dr. Iwan Setiawan Adji, SpTHT-KL, RSUD Karanganyar, Jl. Laksda YosSudarso, Kel Bejen, Kec, Karanganyar. Jawa Tengah. Email: [email protected].

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