scholarly journals Effect of organic load of refinery wastewater in municipal wastewater treatment using bench scale extended aeration activated sludge system

2011 ◽  
Author(s):  
M. A. R. M. M. Amin ◽  
S. R. M. Kutty ◽  
M. H. Isa
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Organic Load ◽  
Municipal Wastewater Treatment ◽  
Refinery Wastewater ◽  
Bench Scale ◽  
Activated Sludge System

scholarly journals Impact of Operational Parameters on Bacterial Community in a Full-Scale Municipal Wastewater Treatment Plant

2012 ◽  
Vol 61 (1) ◽  
pp. 41-49 ◽  
Author(s):  
AGNIESZKA CYDZIK-KWIATKOWSKA ◽  
MAGDALENA ZIELIŃSKA ◽  
IRENA WOJNOWSKA-BARYŁA
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Organic Load ◽  
Municipal Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate ◽  
Ammonia Oxidising Bacteria

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

scholarly journals Characterization of the microbial communities responsible for ammonia oxidation in activated sludge systems of municipal wastewater treatment plants

2021 ◽  
Author(s):  
GM Itheshamul Islam
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Community Composition ◽  
Municipal Wastewater ◽  
Ammonia Oxidation ◽  
Amoa Gene ◽  
Ammonia Oxidizing Bacteria ◽  
Municipal Wastewater Treatment ◽  
Operational Parameters ◽  
Activated Sludge System

Nitrification is an essential microbial process in the global nitrogen cycle. The first step of nitrification is ammonia oxidation which is achieved by bacteria and archaea and is crucial in decreasing ammonia concentrations that are persistently high in wastewater. This study examined the composition, abundance and identity of the microbial community in activated sludge with a focus on characterizing ammonia oxidizing bacteria and archaea in a full-scale municipal wastewater treatment plant (MWTP). Specifically, two pharmaceutical compounds Tetracycline and Ibuprofen, and their effects on the community composition of bacteria and protozoa in activated sludge was investigated using PCR coupled with denaturing gradient gel electrophoresis (DGGE). In addition, the composition, abundance and activity of the ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) were analyzed from aerobic activated sludge, recycled sludge and anaerobic digesters of the Humber MWTP using molecular techniques such as PCR, Quantitative PCR, Reverse Transcription-PCR and DGGE. The findings demonstrated that Tetracycline did not appear to alter community composition of bacteria in the activated sludge, rather, the operational parameters of the sequencing batch reactors such as feeding rates and SRT have shown to alter the richness of bacterial communities. However, Ibuprofen affected some members in the protozoan community in activated sludge. In the full-scale Humber MWTP using the conventional activated sludge system, the aeration tanks contained 1.8 × 105 copies of the AOB amoA gene per 100 ng of DNA. In contrast, the anaerobic digester tanks contained 7.3 × 102 copies of the AOA amoA gene per 100ng of DNA. This study also found that AOB were dominant in activated sludge samples, regardless of the operational parameters. The quantification of cDNA transcripts of the amoA gene also indicated that AOB may be more active than AOA in the activated sludge system. Overall, it appears that AOA are very niche specific and thrive in very low oxygenated environments, while AOB proliferate and play a major role in aerobic ammonia oxidation occurring in MWTPs.

scholarly journals Characterization of the microbial communities responsible for ammonia oxidation in activated sludge systems of municipal wastewater treatment plants

2021 ◽  
Author(s):  
GM Itheshamul Islam
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Community Composition ◽  
Municipal Wastewater ◽  
Ammonia Oxidation ◽  
Amoa Gene ◽  
Ammonia Oxidizing Bacteria ◽  
Municipal Wastewater Treatment ◽  
Operational Parameters ◽  
Activated Sludge System

Nitrification is an essential microbial process in the global nitrogen cycle. The first step of nitrification is ammonia oxidation which is achieved by bacteria and archaea and is crucial in decreasing ammonia concentrations that are persistently high in wastewater. This study examined the composition, abundance and identity of the microbial community in activated sludge with a focus on characterizing ammonia oxidizing bacteria and archaea in a full-scale municipal wastewater treatment plant (MWTP). Specifically, two pharmaceutical compounds Tetracycline and Ibuprofen, and their effects on the community composition of bacteria and protozoa in activated sludge was investigated using PCR coupled with denaturing gradient gel electrophoresis (DGGE). In addition, the composition, abundance and activity of the ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) were analyzed from aerobic activated sludge, recycled sludge and anaerobic digesters of the Humber MWTP using molecular techniques such as PCR, Quantitative PCR, Reverse Transcription-PCR and DGGE. The findings demonstrated that Tetracycline did not appear to alter community composition of bacteria in the activated sludge, rather, the operational parameters of the sequencing batch reactors such as feeding rates and SRT have shown to alter the richness of bacterial communities. However, Ibuprofen affected some members in the protozoan community in activated sludge. In the full-scale Humber MWTP using the conventional activated sludge system, the aeration tanks contained 1.8 × 105 copies of the AOB amoA gene per 100 ng of DNA. In contrast, the anaerobic digester tanks contained 7.3 × 102 copies of the AOA amoA gene per 100ng of DNA. This study also found that AOB were dominant in activated sludge samples, regardless of the operational parameters. The quantification of cDNA transcripts of the amoA gene also indicated that AOB may be more active than AOA in the activated sludge system. Overall, it appears that AOA are very niche specific and thrive in very low oxygenated environments, while AOB proliferate and play a major role in aerobic ammonia oxidation occurring in MWTPs.

Municipal wastewater treatment in Berlin with deep tanks and flotation for secondary clarification

1996 ◽  
Vol 33 (12) ◽  
pp. 199-210
Author(s):  
R. Gnirss ◽  
A. Peter-Fröhlich ◽  
V. Schmidt
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Energy Requirements ◽  
Municipal Wastewater Treatment ◽  
Space Saving ◽  
Process Energy ◽  
Near Future ◽  
Interesting Alternative ◽  
Pilot Tests

For municipal wastewater treatment, space-saving 10m deep activated sludge tanks are an interesting alternative to conventional tanks of shallow construction. Results from pilot tests in the Berlin-Ruhleben WWTP have shown that the biological P-elimination, nutrification and denitrification processes can be implemented as in shallow tanks. However, the activated sludge did not settle satisfactorily. Flotation was implemented in the process for secondary clarification and in the meanwhile has shown to be advantageous. Tests run over a period of some years with the pilot plant have proven the feasibility of this process. Energy requirements for both systems were found to be approximately the same. A cost estimate based on a preplan revealed a 10% advantage in favour of the 10m WWTP with flotation. For these reasons, one 10m WWTP with flotation for secondary clarification and a capacity of 80,000 m3/d will be built in Berlin in the near future.

PARAMETERS FOR DYNAMIC SIMULATION OF W ASTEW ATER TREATMENT PLANTS WITH HIGH-RATE AND LOW-RATE ACTIVATED SLUDGE TANKS

1994 ◽  
Vol 30 (4) ◽  
pp. 211-214 ◽  
Author(s):  
E. Brands ◽  
M. Liebeskind ◽  
M. Dohmann
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Dynamic Simulation ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
High Rate ◽  
Yield Coefficient ◽  
Municipal Wastewater Treatment ◽  
Municipal Wastewater Treatment Plants ◽  
Low Rate

This study shows a comparison of important parameters for dynamic simulation concerning the highrate and low-rate activated sludge tanks of several municipal wastewater treatment plants. The parameters for the dynamic simulation of the single-stage process are quite well known, but parameters for the high-ratellow-rate activated sludge process are still missi ng, although a considerable number of wastewater treatment plants are designed and operated that way. At present any attempt to simulate their operation is restricted to the second stage due to missing data concerning growth rate, decay rate, yield coefficient and others.

Characterization of wastewater and activated sludge from European municipal wastewater treatment plants using the NUR test

1998 ◽  
Vol 38 (1) ◽  
pp. 303-310 ◽  
Author(s):  
V. Naidoo ◽  
V. Urbain ◽  
C. A. Buckley
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Municipal Wastewater Treatment ◽  
Batch Tests ◽  
Denitrification Kinetics ◽  
Municipal Wastewater Treatment Plants

Denitrification kinetics and wastewater characterization of eight different plants in Europe are discussed. Denitrification batch tests revealed three distinct rates except in the cases of Plaisir, Rostock and Orense where 4 rates were observed. The latter three plants revealed atypical rapid initial rates which were between 7 and 21 mgN/gVSS.h. All denitrification kinetics under non-limiting carbon conditions revealed fast first rates which ranged between 3.0 and 7.3 mgN/gVSS.h. Acetate was used to simulate denitrification kinetics with readily biodegradable COD present. Two subsequent rates were observed. Rates 2 and 3 ranged between 2 and 3 mgN/gVSS.h, and 1 and 2 mgN/gVSS.h, respectively. The RBCOD fraction varied between 10 and 19%, except for one of the plants where the value determined was 7%.

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