scholarly journals FERMENTATION OF SACCHARIDES ISSUED FROM A DAIRY INDUSTRY BY A GENETICALLY MODIFIED STRAIN OF ESCHERICHIA COLI INTO ACETOIN AND 2,3-BUTANEDIOL

2018 ◽  
Author(s):  
DAVID FERNÁNDEZ-GUTIERREZ ◽  
MARC VEILLETTE ◽  
ANTONIO AVALOS RAMIREZ ◽  
ANNE GIROIR-FENDLER ◽  
NATHALIE FAUCHEUX ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Modified ◽  
Dairy Industry

scholarly journals Insect-Mediated Dispersal of the Rhizobacterium Pseudomonas chlororaphis

1998 ◽  
Vol 88 (12) ◽  
pp. 1248-1254 ◽  
Author(s):  
William E. Snyder ◽  
David W. Tonkyn ◽  
Daniel A. Kluepfel
Keyword(s):  
Escherichia Coli ◽  
Adult Stage ◽  
Genetically Modified ◽  
Insect Pest ◽  
Corn Rootworm ◽  
Transmission Route ◽  
Pseudomonas Chlororaphis ◽  
Naturally Occurring ◽  
Southern Corn Rootworm ◽  
Corn Plants

The southern corn rootworm, Diabrotica undecimpunctata subsp. howardi, a common and mobile insect pest, was shown to transmit the rhizobacte-rium Pseudomonas chlororaphis strain L11 between corn plants. Strain L11 has been genetically modified to contain the lacZY genes from Escherichia coli. It can reach high densities on roots and invade the roots and move into the foliage. D. undecimpunctata subsp. howardi became infested with L11 as larvae while feeding on roots of seed-inoculated corn and retained the bacteria through pupation, molting to the adult stage, and emergence from the soil. Bacterial densities on or in the insects increased 100-fold after they fed again as adults on L11-infested foliage. Adults retained the bacteria for at least 2 weeks after last exposure and could transmit L11 to new plants. The likelihood of transmission decreased with time since last exposure to L11, but increased with time spent on the new plants. This research demonstrates that rhizobacteria can escape the rhizosphere by moving in or onto foliage, where they can then be acquired and transmitted by insects. This transmission route may be common among naturally occurring rhizobacteria and facilitate the dispersal of both beneficial and harmful soilborne microorganisms.

scholarly journals Safety evaluation of the food enzyme β‐galactosidase from the genetically modified Escherichia coli NCIMB 30325

EFSA Journal ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Modified ◽  
Safety Evaluation

scholarly journals Protection against Shiga-Toxigenic Escherichia coli by Non-Genetically Modified Organism Receptor Mimic Bacterial Ghosts

2015 ◽  
Vol 83 (9) ◽  
pp. 3526-3533 ◽  
Author(s):  
Adrienne W. Paton ◽  
Austen Y. Chen ◽  
Hui Wang ◽  
Lauren J. McAllister ◽  
Florian Höggerl ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Efficiency ◽  
Genetically Modified ◽  
Genetically Modified Organism ◽  
Gastrointestinal Infections ◽  
Content Type ◽  
Recombinant Bacterium ◽  
Bacterial Ghosts

Shiga-toxigenicEscherichia coli(STEC) causes severe gastrointestinal infections in humans that may lead to life-threatening systemic sequelae, such as the hemolytic uremic syndrome (HUS). Rapid diagnosis of STEC infection early in the course of disease opens a window of opportunity for therapeutic intervention, for example, by administration of agents that neutralize Shiga toxin (Stx) in the gut lumen. We previously developed a recombinant bacterium that expresses a mimic of the Stx receptor globotriaosyl ceramide (Gb3) on its surface through modification of the lipopolysaccharide (A. W. Paton, R. Morona, and J. C. Paton, Nat Med6:265–270, 2000,http://dx.doi.org/10.1038/73111). This construct was highly efficaciousin vivo, protecting mice from otherwise fatal STEC disease, but the fact that it is a genetically modified organism (GMO) has been a barrier to clinical development. In the present study, we have overcome this issue by development of Gb3 receptor mimic bacterial ghosts (BGs) that are not classified as GMOs. Gb3-BGs neutralized Stx1 and Stx2in vitrowith high efficiency, whereas alternative Gb3-expressing non-GMO subbacterial particles (minicells and outer membrane blebs) were ineffective. Gb3-BGs were highly efficacious in a murine model of STEC disease. All mice (10/10) treated with Gb3-BGs survived challenge with a highly virulent O113:H21 STEC strain and showed no pathological signs of renal injury. In contrast, 6/10 mice treated with control BGs succumbed to STEC challenge, and survivors exhibited significant weight loss, neutrophilia, and histopathological evidence of renal damage. Thus, Gb3-BGs offer a non-GMO approach to treatment of STEC infection in humans, particularly in an outbreak setting.

Comparative study of two forms of aro A CP4 gene in Escherichia coli

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Satheesh Natarajan ◽  
Stanislav Stuchlík ◽  
Martina Kukučková ◽  
Veronika Renczésová ◽  
Silvia Vávrová ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Genetically Modified Food ◽  
Truncated Form ◽  
E Coli ◽  
Aroa Gene ◽  
Cp4 Epsps ◽  
Herbicide Glyphosate

AbstractThe enzyme CP4 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19) from Agrobacterium tumefaciens CP4, encoded by the aroA gene, has been used for the construction of genetically modified crops resistant to total herbicide glyphosate. During the study of possible horizontal gene transfer of aroA CP4 gene from genetically modified food in gastrointestinal tract to bacterial community living in the animal gut, we have discovered and characterized truncated form of aroA CP4 within the cloning experiments in Escherichia coli. We have compared properties of the recombinant E. coli strains with both CP4 EPSPS enzyme forms.

Subsidy changes may curb Russia's farming successes

2016 ◽  
Keyword(s):  
Agricultural Production ◽  
Genetically Modified ◽  
Import Substitution ◽  
Dairy Industry ◽  
Swine Flu ◽  
High Yield ◽  
Local Production ◽  
Content Type ◽  
Livestock Sector ◽  
Assistance Programme

Subject Challenges to sustaining agricultural production in Russia. Significance Russia has achieved a record grain harvest this year, maintaining its lead as the world's top wheat exporter. The livestock sector has recorded more modest growth, with pork output threatened by a swine flu outbreak and the dairy industry still waiting for a government assistance programme to materialise. Impacts Import substitution will prompt the development of high-yield, non-genetically modified seed. Egypt and Iran will become increasingly important as grain importers. Food exports to China will gain momentum. The resumption of Turkish fruit and vegetable imports will not curb efforts to increase local production.

scholarly journals Safety evaluation of the food enzyme maltogenic amylase from genetically modified Escherichia coli (strain BLASC)

EFSA Journal ◽  
2019 ◽  
Vol 17 (7) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Modified ◽  
Safety Evaluation ◽  
Coli Strain ◽  
Maltogenic Amylase
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