Research on glycerate kinase and its enzymatic properties

2014 ◽  
Author(s):  
Xiangqun Xu ◽  
Yanyan Gao ◽  
Yunqian Cui ◽  
Bo Liu
Keyword(s):  
Enzymatic Properties ◽  
Glycerate Kinase

A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties

2009 ◽  
Vol 31 (12) ◽  
pp. 1937-1941 ◽  
Author(s):  
Bo Liu ◽  
Lei Wu ◽  
Tianming Liu ◽  
Ye Hong ◽  
Yulong Shen ◽  
...  
Keyword(s):  
Enzymatic Properties ◽  
Glycerate Kinase ◽  
Sulfolobus Tokodaii

Enzymatic properties of mouse 25-hydroxyvitamin D3 1α-hydroxylase expressed in Escherichia coli

2001 ◽  
Vol 259 (3) ◽  
pp. 731-738 ◽  
Author(s):  
Toshiyuki Sakaki ◽  
Natsumi Sawada ◽  
Ken-ichi Takeyama ◽  
Shigeaki Kato ◽  
Kuniyo Inouye
Keyword(s):  
Escherichia Coli ◽  
Enzymatic Properties

scholarly journals Adult human liver contains intermediate-type proteasomes with different enzymatic properties

2014 ◽  
Vol 13 (4) ◽  
pp. 429-438 ◽  
Author(s):  
Sabrina Gohlke ◽  
Alexander Kloβ ◽  
Michael Tsokos ◽  
Kathrin Textoris-Taube ◽  
Christin Keller ◽  
...  
Keyword(s):  
Human Liver ◽  
Enzymatic Properties ◽  
Intermediate Type ◽  
Adult Human

scholarly journals Enzymatic Properties of a β-Glucanase from Bacillus subtilis

1961 ◽  
Vol 236 (11) ◽  
pp. 2858-2862
Author(s):  
Ezio A. Moscatelli ◽  
Edward A. Ham ◽  
Edward L. Rickes
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Properties

scholarly journals Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity

2021 ◽  
Vol 22 (13) ◽  
pp. 7205
Author(s):  
Matheus V. C. Grahl ◽  
Augusto F. Uberti ◽  
Valquiria Broll ◽  
Paula Bacaicoa-Caruso ◽  
Evelin F. Meirelles ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Stone Formation ◽  
Cell Types ◽  
Bioinformatic Analysis ◽  
Urinary Stone ◽  
Enzymatic Properties ◽  
Hek293 Cells ◽  
Urinary Stones ◽  
Isolated Cells ◽  
Tnf Α

Infection by Proteus mirabilis causes urinary stones and catheter incrustation due to ammonia formed by urease (PMU), one of its virulence factors. Non-enzymatic properties, such as pro-inflammatory and neurotoxic activities, were previously reported for distinct ureases, including that of the gastric pathogen Helicobacter pylori. Here, PMU was assayed on isolated cells to evaluate its non-enzymatic properties. Purified PMU (nanomolar range) was tested in human (platelets, HEK293 and SH-SY5Y) cells, and in murine microglia (BV-2). PMU promoted platelet aggregation. It did not affect cellular viability and no ammonia was detected in the cultures’ supernatants. PMU-treated HEK293 cells acquired a pro-inflammatory phenotype, producing reactive oxygen species (ROS) and cytokines IL-1β and TNF-α. SH-SY5Y cells stimulated with PMU showed high levels of intracellular Ca2+ and ROS production, but unlike BV-2 cells, SH-SY5Y did not synthesize TNF-α and IL-1β. Texas Red-labeled PMU was found in the cytoplasm and in the nucleus of all cell types. Bioinformatic analysis revealed two bipartite nuclear localization sequences in PMU. We have shown that PMU, besides urinary stone formation, can potentially contribute in other ways to pathogenesis. Our data suggest that PMU triggers pro-inflammatory effects and may affect cells beyond the renal system, indicating a possible role in extra-urinary diseases.

scholarly journals THE ENZYMATIC PROPERTIES OF ISOCITRIC DEHYDROGENASE

1957 ◽  
Vol 226 (2) ◽  
pp. 977-991 ◽  
Author(s):  
Guünther Siebert ◽  
M. Carsiotis ◽  
G.W.E. Plaut
Keyword(s):  
Enzymatic Properties ◽  
Isocitric Dehydrogenase
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