scholarly journals Set-up of a dynamic in vitro exposure device for the study of indoor air pollutant effects on human derived cells

2006 ◽  
Author(s):  
F. Pariselli ◽  
M. G. Sacco ◽  
D. Rembges
Keyword(s):  
Indoor Air ◽  
Air Pollutant ◽  
In Vitro Exposure ◽  
Set Up

scholarly journals New Approach Methods to Evaluate Health Risks of Air Pollutants: Critical Design Considerations for In Vitro Exposure Testing

2020 ◽  
Vol 17 (6) ◽  
pp. 2124 ◽  
Author(s):  
Jose Zavala ◽  
Anastasia N. Freedman ◽  
John T. Szilagyi ◽  
Ilona Jaspers ◽  
John F. Wambaugh ◽  
...  
Keyword(s):  
Air Pollution ◽  
Lessons Learned ◽  
Air Pollutant ◽  
In Vivo Studies ◽  
Design Parameters ◽  
Vast Number ◽  
New Approach ◽  
In Vitro Exposure

Air pollution consists of highly variable and complex mixtures recognized as major contributors to morbidity and mortality worldwide. The vast number of chemicals, coupled with limitations surrounding epidemiological and animal studies, has necessitated the development of new approach methods (NAMs) to evaluate air pollution toxicity. These alternative approaches include in vitro (cell-based) models, wherein toxicity of test atmospheres can be evaluated with increased efficiency compared to in vivo studies. In vitro exposure systems have recently been developed with the goal of evaluating air pollutant-induced toxicity; though the specific design parameters implemented in these NAMs-based studies remain in flux. This review aims to outline important design parameters to consider when using in vitro methods to evaluate air pollutant toxicity, with the goal of providing increased accuracy, reproducibility, and effectiveness when incorporating in vitro data into human health evaluations. This review is unique in that experimental considerations and lessons learned are provided, as gathered from first-hand experience developing and testing in vitro models coupled to exposure systems. Reviewed design aspects include cell models, cell exposure conditions, exposure chambers, and toxicity endpoints. Strategies are also discussed to incorporate in vitro findings into the context of in vivo toxicity and overall risk assessment.

scholarly journals Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an In Vitro Exposure System

2010 ◽  
Vol 115 (2) ◽  
pp. 453-461 ◽  
Author(s):  
Stacey E. Anderson ◽  
Laurel G. Jackson ◽  
Jennifer Franko ◽  
J. R. Wells
Keyword(s):  
Indoor Air ◽  
Exposure System ◽  
In Vitro Exposure

Comparison of sampling methods of indoor air particulate matter for in vitro exposure studies

2017 ◽  
Author(s):  
Maria-Elisa Nordberg ◽  
Martin Täubel ◽  
Kaisa Jalkanen ◽  
Anne Hyvärinen ◽  
Kati Huttunen
Keyword(s):  
Particulate Matter ◽  
Indoor Air ◽  
Sampling Methods ◽  
Air Particulate Matter ◽  
Air Particulate ◽  
In Vitro Exposure

A Heparin-coated Circuit Maintains Platelet Aggregability in Response to Shear Stress in an In Vitro Model of Cardiopulmonary Bypass

1998 ◽  
Vol 80 (09) ◽  
pp. 437-442 ◽  
Author(s):  
I. Hioki ◽  
K. Onoda ◽  
T. Shimono ◽  
H. Shimpo ◽  
K. Tanaka ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cardiopulmonary Bypass ◽  
Membrane Skeleton ◽  
Platelet Glycoprotein ◽  
Platelet Surface ◽  
Platelet Aggregability ◽  
Vitro Model ◽  
Platelet Defects ◽  
Set Up

SummaryAlterations in platelet aggregability may play a role in the pathogenesis of qualitative platelet defects associated with cardiopulmonary bypass (CPB). We circulated fresh heparinized whole blood through tubing sets coated with heparin (C group, n = 10) and through non-coated sets (N group, n = 10) as a simulated CPB circuit. Shear stress (108 dyne/cm2)-induced platelet aggregation (hSIPA), plasma von Willebrand factor (vWF) activity and platelet glycoprotein (GP) Ib expression were measured, before, during, and after this in vitro set up of circulation. In the two groups, the extent of hSIPA significantly decreased during circulation and was partially restored after circulation. Decreases in the extent of hSIPA were significantly less with use of heparin-coated circuits. There was an equivalent reduction in plasma vWF activity, in the two groups. Expression of platelet surface GP Ib decreased significantly during circulation and recovered after circulation. Reduction of surface GP Ib expression during circulation was significantly less in the C group than that in the N group. Decrease in surface GP Ib expression correlated (r = 0.88 in either group) with the magnitude of hSIPA, in the two groups. The progressive removal of surface GP Ib was mainly attributed to redistribution of GP Ib from the membrane skeleton into the cytoskeleton. Our observations suggest that use of heparin-coated circuits partly blocks the reduction of hSIPA, as a result of a lesser degree of redistribution of GP Ib.

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

Plant Biotechnology Workshop for High School Students

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 504e-504
Author(s):  
Erika Szendrak ◽  
Paul E. Read ◽  
Jon S. Miller
Keyword(s):  
High School ◽  
High School Students ◽  
Medical Science ◽  
Plant Biotechnology ◽  
Plant Science ◽  
High School Biology ◽  
School Students ◽  
Subsequent Transformation ◽  
Set Up

Modern aspects of many subjects (e.g., computer science and some aspects of medical science) are now taught in many high schools, but the plant sciences are often given short shrift. A collaboration was therefore established with a high school biology program in which pilot workshops could be developed to enable advanced students to gain insights into modern plant science techniques. A successful example is the workshop on plant biotechnology presented in this report. This workshop is simple and flexible, taking into account that most high school biology laboratories and classrooms are not set up for sophisticated plant science/biotechnology projects. It is suitable for from 10 to 30 students, depending upon space and facilities available. Students work in pairs or trios, and learn simple disinfestation and transfer techniques for micropropagation and potential subsequent transformation treatments. Students gain insights into: sterile technique and hygiene; plant hormones and their physiological effects; plant cell, tissue and organ culture; the influence of environmental factors on response of cells and tissues cultured in vitro; and an understanding of the phenomenon of organogenesis and resulting plant growth and development. This workshop has been tested on several classes of students and following analysis, several refinements were included in subsequent iterations. Results of the students' experiments have been positive and instructive, with student learning outcomes above expectations. Further details of the workshop techniques and approach will be presented.

scholarly journals Ambient air pollutant exposure and in vitro fertilization treatment outcomes in Zhengzhou, China

2021 ◽  
Vol 214 ◽  
pp. 112060
Author(s):  
Wei Dai ◽  
Hao Shi ◽  
Zhiqin Bu ◽  
Yiping Yu ◽  
Zhimin Sun ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Treatment Outcomes ◽  
Ambient Air ◽  
Air Pollutant ◽  
Vitro Fertilization

Alterations in the non-neuronal cholinergic system induced by in-vitro exposure to diazoxon in spleen mononuclear cells of Nile tilapia (O. niloticus)

2021 ◽  
Vol 108 ◽  
pp. 134-141
Author(s):  
G.A. Toledo-Ibarra ◽  
M.I. Girón-Pérez ◽  
C.E. Covantes-Rosales ◽  
G.H. Ventura-Ramón ◽  
G. Pérez-Sánchez ◽  
...  
Keyword(s):  
Cholinergic System ◽  
Nile Tilapia ◽  
Mononuclear Cells ◽  
In Vitro Exposure
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