A technique for development of SSR marker in flowering Chinese cabbage

2014 ◽  
Author(s):  
R.H. Li ◽  
Y.S. Xia ◽  
Y. Li ◽  
L.G. Xu ◽  
H. Zhang ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Ssr Marker ◽  
Flowering Chinese Cabbage

scholarly journals Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Waqas Ahmed ◽  
Yanshi Xia ◽  
Hua Zhang ◽  
Ronghua Li ◽  
Guihua Bai ◽  
...  
Keyword(s):  
Heat Stress ◽  
Abiotic Stress ◽  
High Throughput ◽  
Chinese Cabbage ◽  
Stress Responses ◽  
High Throughput Sequencing ◽  
Differential Expression Analysis ◽  
Plant Responses ◽  
Limited Information ◽  
Flowering Chinese Cabbage

Abstract Plant microRNAs (miRNAs) are noncoding and endogenous key regulators that play significant functions in regulating plant responses to stress, and plant growth and development. Heat stress is a critical abiotic stress that reduces the yield and quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee). However, limited information is available on whether miRNAs are involved in the regulation of heat stress in B. campestris. A high-throughput sequencing approach was used to identify novel and conserved heat-responsive miRNAs in four small RNA libraries of flowering Chinese cabbage using leaves collected at 0 h, 1 h, 6 h and 12 h after a 38 °C heat-stress treatment. The analysis identified 41 conserved miRNAs (belonging to 19 MIR families), of which MIR156, MIR159, MIR168, MIR171 and MIR1885 had the most abundant molecules. Prediction and evaluation of novel miRNAs using the unannotated reads resulted in 18 candidate miRNAs. Differential expression analysis showed that most of the identified miRNAs were downregulated in heat-treated groups. To better understand functional importance, bioinformatic analysis predicted 432 unique putative target miRNAs involved in cells, cell parts, catalytic activity, cellular processes and abiotic stress responses. Furthermore, the Kyoto Encyclopedia of Genes and Genomes maps of flowering Chinese cabbage identified the significant role of miRNAs in stress adaptation and stress tolerance, and in several mitogen-activated protein kinases signaling pathways including cell death. This work presents a comprehensive study of the miRNAs for understanding the regulatory mechanisms and their participation in the heat stress of flowering Chinese cabbage.

Characterization of interspecific hybrids between flowering Chinese cabbage and broccoli

2018 ◽  
Vol 240 ◽  
pp. 552-557 ◽  
Author(s):  
Yunxiao Wei ◽  
Mingzhao Zhu ◽  
Haiyun Qiao ◽  
Fei Li ◽  
Shujiang Zhang ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Interspecific Hybrids ◽  
Flowering Chinese Cabbage

Effect of Color Shading Nets on Growth and Nutrient Uptake of Flowering Chinese Cabbage

2012 ◽  
Vol 461 ◽  
pp. 3-6
Author(s):  
Shi Wei Song ◽  
Qiu Yan Yan ◽  
Fei Dong ◽  
Hou Cheng Liu ◽  
Guang Wen Sun ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutrient Uptake ◽  
Chinese Cabbage ◽  
Vegetable Production ◽  
Fresh Weight ◽  
Flowering Chinese Cabbage ◽  
Nutrients Uptake ◽  
P Content ◽  
Leaf Vegetable ◽  
Color Shading

The effect of color shading-nets (red net, blue net and silver net) and ordinary black net on plant growth and nutrient uptake of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) were studied, with no net shading as the control. The results indicated that, compared with the control, red net covering increased plant height, leaf area and stem diameter of flowering Chinese cabbage. Red and blue nets covering enhanced the above ground fresh weight, while the silver and black nets covering decreased the above ground fresh weight. Shading net covering significantly improved the nitrogen (N) and potassium (K) content of flowering Chinese cabbage, while it had no significant effect on the phosphorus (P) content. Red and blue nets covering enhanced mineral nutrients uptake, while it was decreased under silver and black nets covering. Red and blue nets covering promoted plant growth and nutrients uptake of flowering Chinese cabbage and could be widely applied in leaf vegetable production.

scholarly journals Identification and Analysis of Small Interfering RNAs Associated With Heat Stress in Flowering Chinese Cabbage Using High-Throughput Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Waqas Ahmed ◽  
Yanshi Xia ◽  
Ronghua Li ◽  
Hua Zhang ◽  
Kadambot H.M Siddique ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Tolerance ◽  
Chinese Cabbage ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Small Interfering Rnas ◽  
Flowering Chinese Cabbage ◽  
Disease Resistance Protein ◽  
Temperature Stimulus ◽  
Tolerance Gene

Endogenous small interfering RNAs (siRNAs) are substantial gene regulators in eukaryotes and play key functions in plant development and stress tolerance. Among environmental factors, heat is serious abiotic stress that severely influences the productivity and quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee). However, how siRNAs are involved in regulating gene expression during heat stress is not fully understood in flowering Chinese cabbage. Combining bioinformatical and next-generation sequencing approaches, we identified heat-responsive siRNAs in four small RNA libraries of flowering Chinese cabbage using leaves collected at 0, 1, 6, and 12 h after a 38°C heat-stress treatment; 536, 816, and 829 siRNAs exhibited substantial differential expression at 1, 6, and 12 h, respectively. Seventy-five upregulated and 69 downregulated differentially expressed siRNAs (DE-siRNAs) were common for the three time points of heat stress. We identified 795 target genes of DE-siRNAs, including serine/threonine-protein kinase SRK2I, CTR1-like, disease resistance protein RML1A-like, and RPP1, which may play a role in regulating heat tolerance. Gene ontology showed that predictive targets of DE-siRNAs may have key roles in the positive regulation of biological processes, organismal processes, responses to temperature stimulus, signaling, and growth and development. These novel results contribute to further understanding how siRNAs modulate the expression of their target genes to control heat tolerance in flowering Chinese cabbage.

Sign in / Sign up

Export Citation Format

Share Document