scholarly journals Cellulolytic Bacillus May or May Not Produce β -Glucosidase Due to Their Environmental Origin – A Case Study

2017 ◽  
Vol 7 (6) ◽  
pp. 30
Author(s):  
Lutfun Neesa ◽  
Nasrin Jahan ◽  
Md. Abdullah Al Noman Khan ◽  
Mohammad Shahedur Rahman

Microbial cellulases have been drawing attention worldwide because of their massive capacity to process the most abundant cellulosic biomass into sustainable biofuels and other valuable products. Profitable biomass conversion processes are highly dependent on the use of efficient enzymes for lignocellulose degradation. Among the cellulose degrading enzymes, β-glucosidases are essential for efficient hydrolysis of cellulosic biomass as they relieve the inhibition of the cellobiohydrolases and endoglucanases by reducing cellobiose accumulation. In this study cellulolytic bacteria with potential β-glucosidases activity were isolated and screened from biogas plant effluent and dairy effluent near Jahangirnagar University campus. From initial screening a total of 16 isolates were found to have cellulolytic activity, among them three isolates (B1, B5, D4) were selected based on their superior results. All the three bacterial isolates were identified as B. subtilis (B1), Bacillus amyloliquefaciens (B5) and B. subtilis (D4) respectively based on their morphological, biochemical and molecular characteristics. The β-glucosidases activity of these three potential cellulolytic bacteria was performed by measuring the release of PNP using pNPG as a substrate and interestingly D4 strain was resulted with β-glucosidases negative where B1 strain was found to have efficient for β-glucosidases activity.

2021 ◽  
Vol 13 (15) ◽  
pp. 8238
Author(s):  
Noemi Bakos ◽  
Rosa Schiano-Phan

To transform the negative impacts of buildings on the environment into a positive footprint, a radical shift from the current, linear ‘make-use-dispose’ practice to a closed-loop ‘make-use-return’ system, associated with a circular economy, is necessary. This research aims to demonstrate the possible shift to a circular construction industry by developing the first practical framework with tangible benchmarks for a ‘Circular University Campus’ based on an exemplary case study project, which is a real project development in India. As a first step, a thorough literature review was undertaken to demonstrate the social, environmental and economic benefits of a circular construction industry. As next step, the guideline for a ‘Circular University Campus’ was developed, and its applicability tested on the case study. As final step, the evolved principles were used to establish ‘Project Specific Circular Building Indicators’ for a student residential block and enhance the proposed design through bioclimatic and regenerative design strategies. The building’s performance was evaluated through computational simulations, whole-life carbon analysis and a circular building assessment tool. The results demonstrated the benefits and feasibility of bioclimatic, regenerative building and neighbourhood design and provided practical prototypical case study and guidelines which can be adapted by architects, planners and governmental institutions to other projects, thereby enabling the shift to a restorative, circular construction industry.


Author(s):  
Kara G. Cafferty ◽  
David J. Muth ◽  
Jacob J. Jacobson ◽  
Kenneth M. Bryden

Engineering feedstock supply systems that deliver affordable, high-quality biomass remains a challenge for the emerging bioenergy industry. Cellulosic biomass is geographically distributed and has diverse physical and chemical properties. Because of this feedstock supply systems that deliver cellulosic biomass resources to biorefineries require integration of a broad set of engineered unit operations. These unit operations include harvest and collection, storage, preprocessing, and transportation processes. Design decisions for each feedstock supply system unit operation impact the engineering design and performance of the other system elements. These interdependencies are further complicated by spatial and temporal variances such as climate conditions and biomass characteristics. This paper develops an integrated model that couples a SQL-based data management engine and systems dynamics models to design and evaluate biomass feedstock supply systems. The integrated model, called the Biomass Logistics Model (BLM), includes a suite of databases that provide 1) engineering performance data for hundreds of equipment systems, 2) spatially explicit labor cost datasets, and 3) local tax and regulation data. The BLM analytic engine is built in the systems dynamics software package Powersim™. The BLM is designed to work with thermochemical and biochemical based biofuel conversion platforms and accommodates a range of cellulosic biomass types (i.e., herbaceous residues, short-rotation woody and herbaceous energy crops, woody residues, algae, etc.). The BLM simulates the flow of biomass through the entire supply chain, tracking changes in feedstock characteristics (i.e., moisture content, dry matter, ash content, and dry bulk density) as influenced by the various operations in the supply chain. By accounting for all of the equipment that comes into contact with biomass from the point of harvest to the throat of the conversion facility and the change in characteristics, the BLM evaluates economic performance of the engineered system, as well as determining energy consumption and green house gas performance of the design. This paper presents a BLM case study delivering corn stover to produce cellulosic ethanol. The case study utilizes the BLM to model the performance of several feedstock supply system designs. The case study also explores the impact of temporal variations in climate conditions to test the sensitivity of the engineering designs. Results from the case study show that under certain conditions corn stover can be delivered to the cellulosic ethanol biorefinery for $35/dry ton.


Procedia CIRP ◽  
2016 ◽  
Vol 40 ◽  
pp. 268-273 ◽  
Author(s):  
Vikrant Bhakar ◽  
D.N.S. Hemanth Kumar ◽  
Nitin Krishna Sai ◽  
Kuldip Singh Sangwan ◽  
Smita Raghuvanshi

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Liane Dalla Gasperina ◽  
Janaina Mazutti ◽  
Luciana Londero Brandli ◽  
Roberto dos Santos Rabello

Purpose Smart campuses can be seen as the future of higher education efforts, especially for their contributions to sustainability and to encourage innovation. This paper aims to present the benefits of smart practices in a Higher Education Institutions and highlights its connections to the sustainable development goals (SDGs). Design/methodology/approach The methodology is divided into three steps: first, an international search and assessment of smart practices at universities; second, the identification of smart practices in a university campus in southern Brazil; and third, the presentation of the benefits of smart practices and their relationship with the SDGs. Findings The results showed that globally, the area most covered by smart practices in universities is the environment and, specifically, focused on waste reduction. in the context of this case study, the benefits of implementing smart practices mainly reach SDGs 4 and SDG 9, especially due to aspects of teaching technologies for the new classroom models and the optimization of campus infrastructure management. Practical implications The study encourages other universities to implement smart practices in their campuses, to becoming smart campuses while they also collaborate in achieving the SDGs while raising the discussion on the importance of committed actions taken on a university campus with the UN SDGs, to leverage synergies on campus operations at universities. Originality/value This paper presents a set of smart practices that universities are applying both globally and locally (in southern Brazil). In addition, it contributes to sustainability research by showing how smart practices have the potential to promote SDGs in universities, especially through campus operations.


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