scholarly journals Role of Dynamic GPCR Signaling During Germinal Matrix Blood Vessel Development

2017 ◽  
Vol 1 (Special Issue) ◽  
pp. 164-164
Author(s):  
Devi Santhosh ◽  
Zhen Huang
Keyword(s):  
Blood Vessel ◽  
Germinal Matrix ◽  
Gpcr Signaling ◽  
Blood Vessel Development ◽  
Vessel Development

Role of tissue factor in embryonic blood vessel development

Nature ◽  
1996 ◽  
Vol 383 (6595) ◽  
pp. 73-75 ◽  
Author(s):  
Peter Carmeliet ◽  
Nigel Mackman ◽  
Lieve Moons ◽  
Thomas Luther ◽  
Pierre Gressens ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Tissue Factor ◽  
Blood Vessel Development ◽  
Vessel Development

scholarly journals Activation of Blood Vessel Development in Endometrial Stromal Cells In Vitro Cocultured with Human Peri-Implantation Embryos Revealed by Single-Cell RNA-Seq

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 367
Author(s):  
Bo Lv ◽  
Xiaoyu Xu ◽  
Xunyi Zhang ◽  
Lingbin Qi ◽  
Wen He ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Single Cell ◽  
Stromal Cells ◽  
Differentially Expressed Gene ◽  
Trophoblast Cells ◽  
Endometrial Stromal Cells ◽  
Endometrial Cells ◽  
Blood Vessel Development ◽  
Vessel Development

In humans, the maternal endometrium participates in the physical and physiological interaction with the blastocyst to begin implantation. A bidirectional crosstalk is critical for normal implantation and then a successful pregnancy. While several studies have used animal models or cell lines to study this step, little knowledge was acquired to address the role of endometrial cells in humans. Here, we analyzed single-cell sequencing data from a previous study including 24 non-coculture endometrial stromal cells (EmSCs) and 57 EmSCs after coculture with embryos. We further explored the transcriptomic changes in EmSCs and their interactions with trophoblast cells after coculture. Differentially expressed gene (DEG) analysis showed 1783 upregulated genes and 569 downregulated genes in the cocultured embryos. Weight gene coexpression network and gene ontology analysis of these DEGs showed a higher expression of RAMP1, LTBP1, and LRP1 in EmSCs after coculture, indicating the enrichment of biological processes in blood vessel development and female pregnancy. These data imply that EmSCs start blood vessel development at the implantation stage. Compared with endometrium data in vivo at the implantation window, key pathways including epithelial cell development and oxygen response were involved at this stage. Further analysis using CellphoneDB shed light on the interactions between EmSCs and embryonic trophoblasts, suggesting the important role of integrins and fibroblast growth factor pathways during implantation. Taken together, our work reveals the synchronization signaling and pathways happening at the implantation stage involving the acquisition of receptivity in EmSCs and the interaction between EmSCs and trophoblast cells.

scholarly journals The role of blood flow and microRNAs in blood vessel development

2011 ◽  
Vol 55 (4-5) ◽  
pp. 419-429 ◽  
Author(s):  
Dong Liu ◽  
Janna Krueger ◽  
Ferdinand Le Noble
Keyword(s):  
Blood Flow ◽  
Blood Vessel ◽  
Blood Vessel Development ◽  
Vessel Development

scholarly journals Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting Angiogenesis

2022 ◽  
Author(s):  
Caitlin R Francis ◽  
Hayle Kincross ◽  
Erich J Kushner
Keyword(s):  
Blood Vessel ◽  
Actin Polymerization ◽  
Tissue Type ◽  
Potential Contribution ◽  
Rab Gtpases ◽  
Sprouting Angiogenesis ◽  
Apicobasal Polarity ◽  
Blood Vessel Development ◽  
Vessel Development ◽  
Sprout Formation

In early blood vessel development, trafficking programs, such as those using Rab GTPases, are tasked with delivering vesicular cargo with high spatiotemporal accuracy. However, the function of many Rab trafficking proteins remain ill-defined in endothelial tissue; therefore, their relevance to blood vessel development is unknown. Rab35 has been shown to play an enigmatic role in cellular behaviors which differs greatly between tissue-type and organism. Importantly, Rab35 has never been characterized for its potential contribution in sprouting angiogenesis; thus, our goal was to map Rab35s primary function in angiogenesis. Our results demonstrate that Rab35 is critical for sprout formation; in its absence apicobasal polarity is entirely lost in vitro and in vivo. To determine mechanism, we systematically explored established Rab35 effectors and show that none are operative in endothelial cells. However, we find that Rab35 partners with DENNd1c, an evolutionarily divergent guanine exchange factor, to localize to actin. Here, Rab35 regulates actin polymerization, which is required to setup proper apicobasal polarity during sprout formation. Our findings establish that Rab35 is a potent regulator of actin architecture during blood vessel development.

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