scholarly journals Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

2018 ◽  
Vol 27 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Kazuko Kikuchi ◽  
Tomoyuki Masuda ◽  
Naoki Fujiwara ◽  
Akiyoshi Kuji ◽  
Hiroyuki Miura ◽  
...  
Keyword(s):  
Neural Crest ◽  
Bone Regeneration ◽  
Ips Cell ◽  
Craniofacial Bone

scholarly journals JAGGED1 Stimulates Cranial Neural Crest Cell Osteoblast Commitment Pathways and Bone Regeneration Independent of Canonical NOTCH Signaling

2020 ◽  
Author(s):  
Archana Kamalakar ◽  
Jay M. McKinney ◽  
Daniel Salinas Duron ◽  
Angelica M. Amanso ◽  
Samir A. Ballestas ◽  
...  
Keyword(s):  
Bone Loss ◽  
Neural Crest ◽  
Bone Regeneration ◽  
Neural Crest Cell ◽  
Clinical Problem ◽  
Canonical Pathway ◽  
Regenerative Therapy ◽  
Cranial Neural Crest ◽  
Craniofacial Bone ◽  
Regenerative Approach

AbstractCraniofacial bone loss is a complex clinical problem with limited regenerative solutions. Currently, BMP2 is used as a bone-regenerative therapy in adults, but in pediatric cases of bone loss, it is not FDA-approved due to concerns of life-threatening inflammation and cancer. Development of a bone-regenerative therapy for children will transform our ability to reduce the morbidity associated with current autologous bone grafting techniques. We discovered that JAGGED1 (JAG1) induces cranial neural crest (CNC) cell osteoblast commitment during craniofacial intramembranous ossification, suggesting that exogenous JAG1 delivery is a potential craniofacial bone-regenerative approach. In this study, we found that JAG1 delivery using synthetic hydrogels containing O9-1 cells, a CNC cell line, into critical-sized calvarial defects in C57BL/6 mice provided robust bone-regeneration. Since JAG1 signals through canonical (Hes1/Hey1) and non-canonical (JAK2) NOTCH pathways in CNC cells, we used RNAseq to analyze transcriptional pathways activated in CNC cells treated with JAG1±DAPT, a NOTCH-canonical pathway inhibitor. JAG1 upregulated expression of multiple NOTCH canonical pathway genes (Hes1), which were downregulated in the presence of DAPT. JAG1 also induced bone chemokines (Cxcl1), regulators of cytoskeletal organization and cell migration (Rhou), signaling targets (STAT5), promoters of early osteoblast cell proliferation (Prl2c2, Smurf1 and Esrra), and, inhibitors of osteoclasts (Id1). In the presence of DAPT, expression levels of Hes1 and Cxcl1 were decreased, whereas, Prl2c2, Smurf1, Esrra, Rhou and Id1 remain elevated, suggesting that JAG1 induces osteoblast proliferation through these non-canonical genes. Pathway analysis of JAG1+DAPT-treated CNC cells revealed significant upregulation of multiple non-canonical pathways, including the cell cycle, tubulin pathway, regulators of Runx2 initiation and phosphorylation of STAT5 pathway. In total, our data show that JAG1 upregulates multiple pathways involved in osteogenesis, independent of the NOTCH canonical pathway. Moreover, our findings suggest that JAG1 delivery using a synthetic hydrogel, is a bone-regenerative approach with powerful translational potential.

scholarly journals iPS cell technologies and their prospect for bone regeneration and disease modeling: A mini review

2017 ◽  
Vol 8 (4) ◽  
pp. 321-327 ◽  
Author(s):  
Maria Csobonyeiova ◽  
Stefan Polak ◽  
Radoslav Zamborsky ◽  
Lubos Danisovic
Keyword(s):  
Bone Regeneration ◽  
Disease Modeling ◽  
Ips Cell ◽  
Cell Technologies

Effect of sex-hormone levels, sex, body mass index and other host factors on human craniofacial bone regeneration with bioactive tricalcium phosphate grafts

Biomaterials ◽  
2017 ◽  
Vol 123 ◽  
pp. 48-62 ◽  
Author(s):  
Christine Knabe ◽  
Aynur Mele ◽  
Peter Herbert Kann ◽  
Barbara Peleska ◽  
Doaa Adel-Khattab ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Bone Regeneration ◽  
Body Mass ◽  
Tricalcium Phosphate ◽  
Sex Hormone ◽  
Host Factors ◽  
Hormone Levels ◽  
Craniofacial Bone

scholarly journals Regenerating Craniofacial Dental Defects With Calcium Phosphate Cement Scaffolds: Current Status and Innovative Scope Review

2021 ◽  
Vol 2 ◽  
Author(s):  
Rashed A. Alsahafi ◽  
Heba Ahmed Mitwalli ◽  
Abdulrahman A. Balhaddad ◽  
Michael D. Weir ◽  
Hockin H. K. Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Calcium Phosphate Cement ◽  
Current Status ◽  
Craniofacial Bone ◽  
Calcium Phosphate Cements ◽  
And Performance

The management and treatment of dental and craniofacial injuries have continued to evolve throughout the last several decades. Limitations with autograft, allograft, and synthetics created the need for more advanced approaches in tissue engineering. Calcium phosphate cements (CPC) are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. This review focuses on the up-to-date performance of calcium phosphate cement (CPC) scaffolds and upcoming promising dental and craniofacial bone regeneration strategies. First, we summarized the barriers encountered in CPC scaffold development. Second, we compiled the most up to date in vitro and in vivo literature. Then, we conducted a systematic search of scientific articles in MEDLINE and EMBASE to screen the related studies. Lastly, we revealed the current developments to effectively design CPC scaffolds and track the enhanced viability and therapeutic efficacy to overcome the current limitations and upcoming perspectives. Finally, we presented a timely and opportune review article focusing on the significant potential of CPC scaffolds for dental and craniofacial bone regeneration, which will be discussed thoroughly. CPC offers multiple capabilities that may be considered toward the oral defects, expecting a future outlook in nanotechnology design and performance.

scholarly journals JAGGED1 stimulates cranial neural crest cell osteoblast commitment pathways and bone regeneration independent of canonical NOTCH signaling

Bone ◽  
2021 ◽  
Vol 143 ◽  
pp. 115657
Author(s):  
Archana Kamalakar ◽  
Jay M. McKinney ◽  
Daniel Salinas Duron ◽  
Angelica M. Amanso ◽  
Samir A. Ballestas ◽  
...  
Keyword(s):  
Neural Crest ◽  
Notch Signaling ◽  
Bone Regeneration ◽  
Neural Crest Cell ◽  
Cranial Neural Crest ◽  
Cranial Neural Crest Cell ◽  
Crest Cell

scholarly journals Stem Cell Property of Postmigratory Cranial Neural Crest Cells and Their Utility in Alveolar Bone Regeneration and Tooth Development

Stem Cells ◽  
2009 ◽  
Vol 27 (4) ◽  
pp. 866-877 ◽  
Author(s):  
Il-Hyuk Chung ◽  
Takayoshi Yamaza ◽  
Hu Zhao ◽  
Pill-Hoon Choung ◽  
Songtao Shi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Crest ◽  
Bone Regeneration ◽  
Alveolar Bone ◽  
Tooth Development ◽  
Neural Crest Cells ◽  
Cranial Neural Crest ◽  
Stem Cell Property ◽  
Cell Property ◽  
Cranial Neural Crest Cells

Neural crest-derived cells in nasal conchae of adult mice contribute to bone regeneration

2021 ◽  
Vol 554 ◽  
pp. 173-178
Author(s):  
Hiroshi Yoshida ◽  
Tetsuo Suzawa ◽  
Yo Shibata ◽  
Masahiro Takahashi ◽  
Ryota Kawai ◽  
...  
Keyword(s):  
Neural Crest ◽  
Bone Regeneration ◽  
Adult Mice
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