scholarly journals In Vivo Response of Osteoblast-like and Odontoblast-like Cells in Intraperitoneal Diffusion Chamber

2005 ◽  
Vol 14 (2) ◽  
pp. 294-295 ◽  
Author(s):  
Andrea Paola Rodriguez ◽  
Hidetsugu Tsujigiwa ◽  
Hitoshi Nagatsuka ◽  
Kazuo Ichikawa ◽  
Atsuhisa Minonishi ◽  
...  
Keyword(s):  
Diffusion Chamber

Propagation of Rauscher Leukemia Virus to Human Lymphoblastoid Cells by in Vivo Diffusion Chamber Cultivation

1973 ◽  
Vol 143 (3) ◽  
pp. 850-853
Author(s):  
I. Miyoshi ◽  
S. Yoshimoto ◽  
T. Tsubota ◽  
S. Fujiwara ◽  
H. Dabasaki ◽  
...  
Keyword(s):  
Leukemia Virus ◽  
Diffusion Chamber ◽  
Lymphoblastoid Cells ◽  
Rauscher Leukemia ◽  
Rauscher Leukemia Virus

scholarly journals Radioautographic Studies of Bone Marrow Lymphocytes in Vivo and in Diffusion Chamber Cultures

Blood ◽  
1964 ◽  
Vol 23 (1) ◽  
pp. 1-17 ◽  
Author(s):  
D. G. OSMOND ◽  
N. B. EVERETT
Keyword(s):  
Bone Marrow ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Tritiated Thymidine ◽  
Diffusion Chamber ◽  
Blood Stream ◽  
Turnover Time ◽  
Diffusion Chambers ◽  
Transitional Cells

Abstract Radioautography with tritiated thymidine has been utilized to examine the turnover rate and origin of small lymphocytes in the bone marrow of the guinea-pig. Very few marrow lymphocytes were initially labeled by a single injection of tritiated thymidine, but thereafter the number of labeled lymphocytes rapidly increased to high maximum levels at 3 days. Analysis of the labeling curves and grain counts indicates that the population of marrow lymphocytes is maintained in a dynamic steady state with an average turnover time of 3 days or less. Suspensions of bone marrow cells were isolated from the circulation within intraperitoneal diffusion chambers after short-term labeling with tritiated thymidine in vivo. Although very few small lymphocytes were labeled when introduced into the diffusion chambers, a considerable percentage became labeled during the subsequent culture period. Tritiated thymidine was also administered intravenously whilst excluded from one hind limb by the application of an occlusive compression bandage for 20 minutes. Very few labeled small lymphocytes were found after 72 hours in the tibial marrow of the initially occluded limb, whereas the normal high percentage was labeled in the control tibial marrow. These experiments do not demonstrate any large-scale influx of small lymphocytes from the blood stream into the marrow parenchyma. They suggest that newly formed small lymphocytes appear in the marrow as a result of the division of locally situated precursor cells, but the mechanism of intramedullary lymphocytopoiesis is uncertain. "Transitional" cells, intermediate in morphology between blast cells and small lymphocytes, synthesize DNA and are actively proliferative, but they do not appear to account fully for the rate of lymphocyte production. Certain large, undifferentiated labeled cells appeared in the bone marrow as a result of hematogenous migration. Some implications of these findings are discussed.

scholarly journals Two classes of murine granulocyte progenitor cells expressed in plasma clot diffusion chamber cultures

Blood ◽  
1982 ◽  
Vol 59 (4) ◽  
pp. 838-843 ◽  
Author(s):  
HN Steinberg ◽  
PL Page ◽  
SH Robinson
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Cytosine Arabinoside ◽  
Diffusion Chamber ◽  
Velocity Sedimentation ◽  
Mouse Bone Marrow ◽  
Plasma Clot ◽  
Residual Concentration

Abstract Two distinct classes of granulocyte progenitor cells present in normal mouse bone marrow are expressed sequentially in the vivo plasma clot diffusion chamber culture system. By several criteria, progenitor cells giving rise to granulocyte colonies on day 4 of culture (CFU-D4) are different from those giving rise to colonies on day 7 (CFU-D7). These differences include: cell cycle activity as measured by in vitro incubation with cytosine arabinoside, residual concentration in the bone marrow after in vivo treatment of donor mice with cytosine arabinoside or methotrexate, resistance to osmotic lysis, size as determined by velocity sedimentation, and the morphology of the granulocyte colonies to which these cells give rise. The CFU-D7 appears to represent an earlier progenitor cell than the CFU-D4 in the differentiation pathway of the granulocyte and is analagous in many respects to the BFU-E in the erythroid pathway.

scholarly journals Granulopoiesis inhibition in acute inflammation: comparative studies in healthy and leukaemic mice

1992 ◽  
Vol 1 (3) ◽  
pp. 171-175
Author(s):  
Mohamad Hamood ◽  
Francis Corazza ◽  
Pierre Francois Bluche ◽  
Hassan El Teraifi ◽  
Pierre Fondu
Keyword(s):  
Acute Inflammation ◽  
Regulatory Mechanism ◽  
Diffusion Chamber ◽  
Colony Growth ◽  
Cell Content ◽  
Humoral Factors ◽  
Leukaemic Cells

It was demonstrated previously that mice undergoing an inflammatory reaction induced by subcutaneous (SC) implantation of copper rods, produce humoral factors that initially enhance, but subsequently inhibit, diffusion chamber (DC) granulopoiesis. This provided evidence that granulopoiesis is under the control of both humoral stimulators and inhibitors. In order to test the granulopoietic regulatory mechanism in leukaemic mice, we investigated the regulatory role of granulopoietic humoral inhibitors during in vivo granulopoiesis. We noticed that mice suffering from acute myeloid leukaemia (AML) are unable to augment the production of these humoral inhibitory factors when acute inflammation is induced, since no change in DC cell content was observed with or without prior inflammation. Moreover, unlike healthy mice, the serum of leukaemic mice withdrawn during the inhibition phase of acute inflammation did not show any inhibitory activity toward granulocyte—monocyte (GM) colony growth in vitro. Our results also show that increased levels of normal humoral inhibitors do not influence the proliferation and/or differentiation of leukaemic cells implanted in diffusion chamber cultures.

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