scholarly journals Cell Dynamics in Tooth Root Formation: Hertwig's Epithelial Root Sheath and Cellular Cementogenesis

2005 ◽  
Vol 14 (2) ◽  
pp. 176-177
Author(s):  
Naoki Fujiwara ◽  
Makoto J. Tabata ◽  
Tokio Nawa
Keyword(s):  
Root Formation ◽  
Tooth Root ◽  
Cell Dynamics ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Immortalized Hertwig's epithelial root sheath cell line works as model for epithelial–mesenchymal interaction during tooth root formation

2019 ◽  
Vol 235 (3) ◽  
pp. 2698-2709 ◽  
Author(s):  
Sicheng Zhang ◽  
Xuebing Li ◽  
Shikai Wang ◽  
Yan Yang ◽  
Weihua Guo ◽  
...  
Keyword(s):  
Cell Line ◽  
Root Formation ◽  
Tooth Root ◽  
Sheath Cell ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Developmental Properties of the Hertwig’s Epithelial Root Sheath in Mice

2004 ◽  
Vol 83 (9) ◽  
pp. 688-692 ◽  
Author(s):  
H. Yamamoto ◽  
S.-W. Cho ◽  
E.-J. Kim ◽  
J.-Y. Kim ◽  
N. Fujiwara ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Culture System ◽  
Root Formation ◽  
Expression Patterns ◽  
Tooth Root ◽  
Comprehensive Model ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath ◽  
In Vitro Culture System

Hertwig’s epithelial root sheath (HERS) plays an important role in tooth root formation. In this study, we examined root formation of the first molar in mice, focusing on cell proliferation, cell death, cell migration, and the expression patterns of the signaling molecules, including glycoproteins and proteoglycans between PN8 and PN26. The number of HERS cells decreased during root formation, although HERS retained total length until PN15. The migration of HERS cells did not occur during root formation. Moreover, the immunopositive reaction of laminin beta-3 and syndecan-1 in HERS indicates that both cell adhesion and cell proliferation are essential for HERS development. Bmp-2, Bmp-4, and Msx-2 were expressed in HERS cells during root formation. We also developed an in vitro culture system for investigating the periodontium and suggest that this system provides an excellent vehicle for full exploration, and hence improved understanding, of the development and regeneration of the periodontium. Together, our results provide a comprehensive model describing the morphogenesis of early root development in vertebrates.

scholarly journals Ameloblastin in Hertwig’s Epithelial Root Sheath Regulates Tooth Root Formation and Development

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54449 ◽  
Author(s):  
Naoto Hirose ◽  
Atsushi Shimazu ◽  
Mineo Watanabe ◽  
Kotaro Tanimoto ◽  
Souichi Koyota ◽  
...  
Keyword(s):  
Root Formation ◽  
Tooth Root ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Sonic Hedgehog Signaling is Important in Tooth Root Development

2006 ◽  
Vol 85 (5) ◽  
pp. 427-431 ◽  
Author(s):  
M. Nakatomi ◽  
I. Morita ◽  
K. Eto ◽  
M.S. Ota
Keyword(s):  
Sonic Hedgehog ◽  
Root Development ◽  
Mutant Cell ◽  
Expression Patterns ◽  
Nuclear Antigen ◽  
Tooth Root ◽  
Shh Signaling ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Hertwig’s epithelial root sheath (HERS) is important for tooth root formation, but the molecular basis for the signaling of root development remains uncertain. We hypothesized that Sonic hedgehog (Shh) signaling is involved in the HERS function, because it mediates epithelial-mesenchymal interactions during embryonic odontogenesis. We examined the gene expression patterns of Shh signaling in murine developing molar roots. Shh and Patched2 transcripts were identified in the HERS, whereas Patched1, Smoothened, and Gli1 were expressed in the proliferative dental mesenchyme in addition to the HERS. To confirm whether Shh signaling physiologically functions in vivo, we analyzed mesenchymal dysplasia ( mes) mice carrying an abnormal C-terminus of the PATCHED1 protein. In the mutant, cell proliferation was repressed around the HERS at 1 wk. Moreover, the molar eruption was disturbed, and all roots were shorter than those in control littermates at 4 wks. These results indicate that Shh signaling is important in tooth root development. Abbreviations used: BrdU, 5-bromo-2′-deoxyuridine; HERS, Hertwig’s epithelial root sheath; NFI-C/CTF, nuclear factor Ic/CAAT box transcription factor; PCNA, proliferating cell nuclear antigen; Ptc, patched; Shh, sonic hedgehog; Smo, smoothened.

Sign in / Sign up

Export Citation Format

Share Document