scholarly journals Interleukin-8 Production by Human Gingival Fibroblasts in Response to Periodontopathic Bacterial Lipopolysaccharides and Prostaglandin E2 In vitro.

2004 ◽  
Vol 13 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Takeo Fujii ◽  
Pao-Li Wang ◽  
Toshihiko Yajima ◽  
Anne Carrel-Geinoz ◽  
Shyuhei Usui ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Bacterial Lipopolysaccharides

scholarly journals Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1382
Author(s):  
Katharina Schueller ◽  
Joachim Hans ◽  
Stefanie Pfeiffer ◽  
Jessica Walker ◽  
Jakob P. Ley ◽  
...  
Keyword(s):  
Cell Model ◽  
Periodontal Diseases ◽  
Oral Care ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Periodontal Inflammation ◽  
Anti Inflammatory ◽  
Related Compounds

Background: In order to identify potential activities against periodontal diseases, eighteen dihydrochalcones and structurally related compounds were tested in an established biological in vitro cell model of periodontal inflammation using human gingival fibroblasts (HGF-1 cells). Methods: Subsequently to co-incubation of HGF-1 cells with a bacterial endotoxin (Porphyromonas gingivalis lipopolysaccharide, pgLPS) and each individual dihydrochalcone in a concentration range of 1 µM to 100 µM, gene expression of interleukin-8 (IL-8) was determined by qPCR and cellular interleukin-8 (IL-8) release by ELISA. Results: Structure–activity analysis based on the dihydrochalcone backbone and various substitution patterns at its aromatic ring revealed moieties 2′,4,4′,6′-tetrahydroxy 3-methoxydihydrochalcone (7) to be the most effective anti-inflammatory compound, reducing the pgLPS-induced IL-8 release concentration between 1 µM and 100 µM up to 94%. In general, a 2,4,6-trihydroxy substitution at the A-ring and concomitant vanilloyl (4-hydroxy-3-methoxy) pattern at the B-ring revealed to be preferable for IL-8 release inhibition. Furthermore, the introduction of an electronegative atom in the A,B-linker chain led to an increased anti-inflammatory activity, shown by the potency of 4-hydroxybenzoic acid N-vanillylamide (13). Conclusions: Our data may be feasible to be used for further lead structure designs for the development of potent anti-inflammatory additives in oral care products.

scholarly journals Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro

2021 ◽  
Author(s):  
Jens Weusmann ◽  
James Deschner ◽  
Jean-Claude Imber ◽  
Anna Damanaki ◽  
Natalia D. P. Leguizamón ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Function ◽  
Nuclear Translocation ◽  
In Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Mrna Levels ◽  
Gingival Fibroblasts ◽  
Air Polishing ◽  
Wide Range

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

Azithromycin suppresses P. gingivalis LPS-induced pro-inflammatory cytokine and chemokine production by human gingival fibroblasts in vitro

2014 ◽  
Vol 19 (2) ◽  
pp. 221-227 ◽  
Author(s):  
C. J. Doyle ◽  
T. R. Fitzsimmons ◽  
C. Marchant ◽  
A. A. S. S. K. Dharmapatni ◽  
R. Hirsch ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Chemokine Production

In Vitro Attachment of Human Gingival Fibroblasts to Root Surfaces

1975 ◽  
Vol 46 (11) ◽  
pp. 639-645 ◽  
Author(s):  
Joseph J. Aleo ◽  
Frank A. De Renzis ◽  
Paul A. Farber
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Root Surfaces

scholarly journals In vitro study of surface alterations to polyetheretherketone and titanium and their effect upon human gingival fibroblasts

2021 ◽  
Vol 125 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Maryam Gheisarifar ◽  
Geoffrey A. Thompson ◽  
Carl Drago ◽  
Fahimeh Tabatabaei ◽  
Morteza Rasoulianboroujeni
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

An In Vitro Evaluation of the Cytotoxicity of Various Endodontic Irrigants On Human Gingival Fibroblasts

2005 ◽  
Vol 31 (8) ◽  
pp. 613-615 ◽  
Author(s):  
B BARNHART ◽  
A CHUANG ◽  
J LUCCA ◽  
S ROBERTS ◽  
F LIEWEHR ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
In Vitro Evaluation
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