scholarly journals Serotonin and neuropeptide FMRFamide in the attachment organs of trematodes

2018 ◽  
Vol 55 (3) ◽  
pp. 185-194 ◽  
Author(s):  
N. B. Terenina ◽  
N. D. Kreshchenko ◽  
N. B. Mochalova ◽  
S. O. Movsesyan
Keyword(s):  
Nervous System ◽  
Muscle Activity ◽  
Confocal Scanning Laser Microscopy ◽  
Muscle Fibres ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Immunocytochemical Techniques ◽  
Confocal Scanning Laser

Summary The serotoninergic and FMRFamidergic nervous system of the attachment organs of trematodes were examined using immunocytochemical techniques and confocal scanning laser microscopy. Adult trematodes from eight families as well as cercariae and metacercariae from ten families were studied. TRITC-conjugated phalloidin was used to stain the muscle fibres. The serotonin- and FMRFamide-immunoreactive (IR) nerve cells and fibres were revealed to be near the muscle fibres of the oral and ventral suckers of the trematodes and their larvae. The results indicate the important role of neurotransmitters, serotonin and neuropeptide FMRFamide in the regulation of muscle activity in the attachment organs of trematodes and can be considered in perspective for the development of new anthelmintic drugs, which can interrupt the function of the attachment organs of the parasites.

Cytochemical observations on cholinergic, serotoninergic and peptidergic neuronal pathways in Cephalochlamys namaquensis

1992 ◽  
Vol 66 (3) ◽  
pp. 205-212 ◽  
Author(s):  
D. M. Mckay ◽  
D. W. Halton ◽  
C. F. Johnston ◽  
C. Shaw ◽  
I. Fairweather
Keyword(s):  
Nervous System ◽  
Nerve Cell ◽  
Similar Pattern ◽  
Regional Differences ◽  
Confocal Scanning Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Neuroactive Substance ◽  
Confocal Scanning ◽  
Immunocytochemical Techniques ◽  
Confocal Scanning Laser

ABSTRACTThe localization and distribution of cholinergic, serotoninergic (5-HT, serotonin) and peptidergic components of the nervous system of adult Cephalochlamys namaquensis (Cestoda: Pseudophyllidea) have been determined using enzyme histochemical and immunocytochemical techniques interfaced with light and confocal scanning laser microscopy. All three classes of neuroactive substance showed a similar pattern of staining, occurring extensively throughout the central and peripheral nervous systems of the parasite. There were some minor regional differences in staining, suggesting specific roles for certain classes of neurone, and nerve cell bodies were most evident following immunostaining for serotonin. The general overlap in the distribution of staining may be indicative of some co-localization of neurotransmitter and/or neuromodulatory substances.

The serotoninergic, cholinergic and peptidergic components of the nervous system in the monogenean parasite,Diclidophora merlangi: a cytochemical study

Parasitology ◽  
1990 ◽  
Vol 100 (2) ◽  
pp. 255-273 ◽  
Author(s):  
A. G. Maule ◽  
D. W. Halton ◽  
C. F. Johnston ◽  
C. Shaw ◽  
I. Fairweather
Keyword(s):  
Nervous System ◽  
Cholinesterase Activity ◽  
Histochemical Staining ◽  
Confocal Scanning Laser Microscopy ◽  
Cytochemical Study ◽  
Monogenean Parasite ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Immunocytochemical Techniques ◽  
Confocal Scanning Laser

SUMMARYConfocal scanning laser microscopy has been employed with immunocytochemical techniques to map the distribution of serotoninergic and peptidergic components in the nervous system of the monogenean gill-parasite,Diclidophora merlangi; results are compared with the distribution of cholinergic components, following histochemical staining for cholinesterase activity. While all three neurochemical elements are present in the central and peripheral nervous systems, the cholinergic and peptidergic systems dominate the CNS, whereas the PNS has a majority of serotoninergic nerve fibres. The cholinergic and peptidergic neuronal pathways overlap extensively in staining patterns, suggesting possible co-localization of acetylcholine and neuropeptides. Within the peptidergic nervous system, immunoreactivity to the pancreatic polypeptide family of peptides and FMRFamide were the most prevalent.

scholarly journals In Vivo Confocal Scanning Laser Microscopy of Human Skin: Melanin Provides Strong Contrast

1995 ◽  
Vol 104 (6) ◽  
pp. 946-952 ◽  
Author(s):  
Milind Rajadhyaksha ◽  
Melanie Grossman ◽  
Dina Esterowitz ◽  
Robert H. Webb ◽  
R Rox Anderson
Keyword(s):  
Human Skin ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Strong Contrast
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