scholarly journals Promising Application of Dynamic Nuclear Polarization for in Vivo 13C MR Imaging

2011 ◽  
Vol 10 (4) ◽  
pp. 211-217 ◽  
Author(s):  
Yi-Fen YEN ◽  
Kiyoshi NAGASAWA ◽  
Tsutomu NAKADA
Keyword(s):  
Mr Imaging ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Promising Application

scholarly journals Dynamic nuclear polarization facilitates monitoring of pyruvate metabolism in Trypanosoma brucei

2017 ◽  
Vol 292 (44) ◽  
pp. 18161-18168 ◽  
Author(s):  
You Zhuo ◽  
Ciro D. Cordeiro ◽  
S. Khan Hekmatyar ◽  
Roberto Docampo ◽  
James H. Prestegard
Keyword(s):  
Trypanosoma Brucei ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Hep G2 ◽  
Hek 293 ◽  
Mammalian Cell Lines ◽  
Microbial Systems ◽  
Viable Method ◽  
Pathogenic Protozoan

Dynamic nuclear polarization provides sensitivity improvements that make NMR a viable method for following metabolic conversions in real time. There are now many in vivo applications to animal systems and even to diagnosis of human disease. However, application to microbial systems is rare. Here we demonstrate its application to the pathogenic protozoan, Trypanosoma brucei, using hyperpolarized 13C1 pyruvate as a substrate and compare the parasite metabolism with that of commonly cultured mammalian cell lines, HEK-293 and Hep-G2. Metabolic differences between insect and bloodstream forms of T. brucei were also investigated. Significant differences are noted with respect to lactate, alanine, and CO2 production. Conversion of pyruvate to CO2 in the T. brucei bloodstream form provides new support for the presence of an active pyruvate dehydrogenase in this stage.

scholarly journals Dynamic nuclear polarization of biocompatible13C-enriched carbonates for in vivo pH imaging

2016 ◽  
Vol 52 (14) ◽  
pp. 3030-3033 ◽  
Author(s):  
D. E. Korenchan ◽  
R. R. Flavell ◽  
C. Baligand ◽  
R. Sriram ◽  
K. Neumann ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Ph Imaging ◽  
Low Toxicity ◽  
High Concentrations ◽  
Rapid Hydrolysis ◽  
Hydrolysis Of

High concentrations of hyperpolarized13C-bicarbonate are generatedviarapid hydrolysis of highly polarizable, low-toxicity carbonate precursors.

Redox metabolic imaging of NASH model mice using in vivo dynamic nuclear polarization MRI

2018 ◽  
Vol 128 ◽  
pp. S86
Author(s):  
Fuminori Hyodo ◽  
Ryosuke Nakata ◽  
Hinako Eto ◽  
Tomoko Nakaji ◽  
Tatsuya Naganuma ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Metabolic Imaging ◽  
Nash Model
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