scholarly journals Evaluation of procoagulant tissue factor expression in canine hemangiosarcoma cell lines

2017 ◽  
Vol 78 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Lauren E. Witter ◽  
Erika J. Gruber ◽  
Fabian Z. X. Lean ◽  
Tracy Stokol
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Tissue Factor Expression ◽  
Factor Expression

Simultaneous tissue factor expression and phosphatidylserine exposure account for the highly procoagulant pattern of melanoma cell lines

2009 ◽  
Vol 19 (5) ◽  
pp. 301-308 ◽  
Author(s):  
Clarice Kirszberg ◽  
Luize G. Lima ◽  
Andreia Da Silva de Oliveira ◽  
William Pickering ◽  
Elaine Gray ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Tissue Factor Expression ◽  
Phosphatidylserine Exposure ◽  
Factor Expression ◽  
Melanoma Cell Lines

scholarly journals Tissue factor associates with survival and regulates tumour progression in osteosarcoma

2016 ◽  
Vol 115 (05) ◽  
pp. 1025-1033 ◽  
Author(s):  
Chris Tieken ◽  
Michiel C. Verboom ◽  
Wolfram Ruf ◽  
Hans Gelderblom ◽  
Judith V. M. G. Bovée ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Lung Metastasis ◽  
Osteosarcoma Cell ◽  
Tissue Factor Expression ◽  
Free Survival ◽  
Matrigel Invasion ◽  
Factor Expression ◽  
Micro Vessel Density

SummaryOsteosarcoma is the most common primary malignant bone tumour. Patients often develop lung metastasis and have a poor prognosis despite extensive chemotherapy and surgical resections. Tissue Factor is associated with poor clinical outcome in a wide range of cancer types, and promotes angiogenesis and metastasis. The role of Tissue Factor in OS tumourigenesis is unknown. Fifty-three osteosarcoma pre-treatment biopsies and four osteosarcoma cell lines were evaluated for Tissue Factor expression, and a possible association with clinical parameters was investigated. Tissue Factor function was inhibited in an osteosarcoma cell line (143B) by shRNA knockdown or specific antibodies, and pro-tumourigenic gene expression, proliferation, matrigel invasion and transwell migration was examined. 143B cells were implanted in mice in the presence of Tissue Factor-blocking antibodies, and tumour volume, micro-vessel density and metastases in the lung were evaluated. Tissue Factor was highly expressed in 73.6 % of osteosarcoma biopsies, and expression associated significantly with disease-free survival. Tissue Factor was expressed in all four investigated cell lines. Tissue Factor was knocked down in 143B cells, which led to reduced expression of IL-8, CXCL-1, SNAIL and MMP2, but not MMP9. Tissue Factor knockdown or inhibition with antibodies reduced matrigel invasion. Tissue Factor antibodies limited 143B tumour growth in vivo, and resulted in decreased intra-tumoural micro-vessel density. Furthermore, lung metastasis from the primary tumour was significantly reduced. Thus, Tissue Factor expression in osteosarcoma reduces metastasis-free survival in patients, and increases pro-tumourigenic behaviour both in vitro and in vivo.Supplementary Material to this article is available online at www.thrombosis-online.com.

The effect of cytokines on tissue factor expression in HL-60 and U937 cell lines

1995 ◽  
Vol 23 (2) ◽  
pp. 286S-286S ◽  
Author(s):  
JENNIFER C. FREEBURN ◽  
WILLIAM S. GILMORE ◽  
JOHN J. STRAIN
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
U937 Cell ◽  
Tissue Factor Expression ◽  
Factor Expression

TISSUE FACTOR EXPRESSION IN HUMAN MONOCYTIC CELL LINES

1997 ◽  
Vol 88 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Taavo Tenno ◽  
Johan Botling ◽  
Fredrik Öberg ◽  
Kenneth Nilsson ◽  
Agneta Siegbahn
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Tissue Factor Expression ◽  
Monocytic Cell ◽  
Factor Expression ◽  
Human Monocytic Cell

The Factor VIII Bypassing Activity of Prothrombin Complex Concentrates: The Roles of Factor VIla and of Endothelial Cell Tissue Factor

1991 ◽  
Vol 66 (05) ◽  
pp. 559-564 ◽  
Author(s):  
Jerome M Teitel
Keyword(s):  
Factor Viii ◽  
Tissue Factor ◽  
Factor Viia ◽  
Factor Xa ◽  
Procoagulant Activity ◽  
Tissue Factor Expression ◽  
Prothrombin Complex Concentrates ◽  
Cell Tissue ◽  
Factor Expression ◽  
Necrosis Factor

SummaryAn experimental model incorporating cultured endothelial cells (EC) was used to study the "factor VIII bypassing" activity of prothrombin complex concentrates (PCC), a property exploited in the treatment of hemophiliacs with alloantibodies to factor VIII. Two PCC preparations were ineffective as stimuli of tissue factor expression by EC. However, incubation with a combination of PCC plus endotoxin (lipopolysaccharide, LPS) or tumor necrosis factor (TNF) induced much greater tissue factor expression than was seen in response to either substance alone. PCC expressed an additional direct procoagulant activity at the EC surface, which could not be attributed to either thrombin or factor Xa, and which was diminished by an anti-tissue factor antibody. Therefore factor VIIa, which was detectable in both PCC preparations, likely provided this additional direct procoagulant activity at the EC surface. We also excluded the possibility that coagulation proteases contained in or generated in the presence of PCC are protected from inactivation by AT III. Therefore, PCC can indirectly bypass factor VIII by enhancing induced endothelial tissue factor expression, and also possess direct procoagulant activity, probably mediated by factor VIIa.

Upregulation of tissue factor expression by avastin: ex vivo and in vitro data

2012 ◽  
Vol 129 ◽  
pp. S170
Author(s):  
E. Napoleone ◽  
A. Cutrone ◽  
D. Cugino ◽  
R. Tambaro ◽  
A. De Curtis ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Ex Vivo ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Vitro Data ◽  
In Vitro Data

Nutritional compounds influence tissue factor expression and inflammation of chronic kidney disease patients in vitro

Nutrition ◽  
2011 ◽  
Vol 27 (9) ◽  
pp. 967-972 ◽  
Author(s):  
Cecilia M. Shing ◽  
Murray J. Adams ◽  
Robert G. Fassett ◽  
Jeff S. Coombes
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Nutritional Compounds
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