scholarly journals A COMPARATIVE ANALYSIS OF PLANT GROWTH-PROMOTING TRAITS OF Pseudomonas AND Bacillus STRAINS ISOLATED FROM Lolium perenne RHIZOSPHERIC SOIL IN VOJVODINA (SERBIA) AND THEIR EFFECT ON THE PLANT YIELD

2020 ◽  
Vol 19 (3) ◽  
pp. 37-45
Author(s):  
Dragana Stamenov ◽  
Timea I. Hajnal-Jafari ◽  
Biljana Najvirt ◽  
Snežana Anđelković ◽  
Jelena Tomić ◽  
...  
Keyword(s):  
Plant Growth ◽  
Lolium Perenne ◽  
Biochemical Characterization ◽  
Total Yield ◽  
Forage Crops ◽  
Vitro Assay ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Impact

The objective of this work was to do a comparative study of Pseudomonas and Bacillus isolates for their plant growth-promoting (PGP) potential, monitoring the impact of selected isolates on the yield of English ryegrass (Lolium perenne). Isolation, physiological and biochemical characterization, in vitro assay of enzymatic and plant-growth promoting activities of isolates were done. Pseudomonas isolates have been shown to have the ability to use different sources of carbon, to live in the condition of low pH as well as temperature and to produce siderophore. On the other hand, Bacillus isolates have the ability to solubilize phosphate, to produce a greater amount of indol-3-acetic acid (IAA) than Pseudomonas isolates and have an inhibitory effect on the growth of phytopathogenic fungi. In other investigated traits, isolates were similar. The use of Pseudomonas sp. P12 and Bacillus sp. B1 isolates had a positive effect on the plant mass and total yield, which indicate that the use of these isolates can result in a better yield of forage crops.

scholarly journals Physiological Adaptation and Plant Growth Promoting Functional Traits of Bacillus altitudinis FD48 under In vitro Osmotic Stress

2020 ◽  
pp. 92-98
Author(s):  
Shobana Narayanasamy ◽  
Sugitha Thankappan ◽  
Sivakumar Uthandi
Keyword(s):  
Plant Growth ◽  
Osmotic Stress ◽  
Functional Traits ◽  
Physiological Adaptation ◽  
Bacillus Altitudinis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Impact ◽  
Plant Growth Promoting Traits

To develop an osmotolerant microbe, as a bioinoculant to mitigate drought it is vital to understand the impact of osmotic stress on their growth and plant growth promoting functional traits. The present study was aimed to evaluate the physiological adaptations and plant growth-promoting traits of a phyllosphere bacterium Bacillus altitudinis FD48 under osmotic stress conditions. The FD48 strain isolated from rice (cultivar ADT43) phyllosphere obtained from Biocatalysts laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore. In vitro bioassay was conducted to evaluate the osmotolerant potentials of FD48. B. altitudinis FD48 grown in LB supplemented with PEG 6000 and grown for 48 hrs. Physiological adaptation to osmotic stress was observed by assessing the osmolytes and free amino acids content produced by FD48 under induced stress. Further the plant growth promoting traits under osmotic stress also asceratined. The growth pattern of FD48 strain decreased with the increase in PEG concentrations. The lower level of osmotic stress enhanced the growth of FD48 but at higher concentration exhibited a decline in growth. Enhanced levels of IAA (25 µg g-1 of protein) and EPS (9.76 mg mg-1 protein) production were recorded in the FD48 strain at lower levels of osmotic stress. Furthermore, an increase in osmotic stress had a deleterious effect on IAA production and ACC deaminase activity while the exopolysaccharide production was enhanced. Growth of FD48 under osmotic stress also increased the accumulation of proline and compatible sugars that will protect the FD48 strain by maintaining the turgor potential of cells and stabilizes the membrane proteins. Hence, the results of our study suggesting that, B. altitudinis FD48 strain has the potential to tolerate osmotic stress and might be used as a newer bio-inoculant for triggering moisture deficit stress resilience in plants.

scholarly journals Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

2021 ◽  
Vol 9 (6) ◽  
pp. 1274
Author(s):  
Leardwiriyakool Chaiya ◽  
Jaturong Kumla ◽  
Nakarin Suwannarach ◽  
Tanongkiat Kiatsiriroat ◽  
Saisamorn Lumyong
Keyword(s):  
Plant Growth ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fruit Production ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Genus Streptomyces ◽  
Plant Growth Promoting ◽  
Growth Promoting

Nowadays, microorganisms that display plant growth promoting properties are significantly interesting for their potential role in reducing the use of chemical fertilizers. This research study proposed the isolation of the actinobacteria associated with arbuscular mycorrhizal fungi (AMF) spores and the investigation of their plant growth promoting properties in the in vitro assay. Three actinobacterial strains were obtained and identified to the genus Streptomyces (GETU-1 and GIG-1) and Amycolatopsis (GLM-2). The results indicated that all actinobacterial strains produced indole-3-acetic acid (IAA) and were positive in terms of siderophore, endoglucanase, and ammonia productions. In the in vitro assay, all strains were grown in the presence of water activity within a range of 0.897 to 0.998, pH values within a range of 5–11, and in the presence of 2.5% NaCl for the investigation of drought, pH, and salt tolerances, respectively. Additionally, all strains were able to tolerate commercial insecticides (propargite and methomyl) and fungicides (captan) at the recommended dosages for field applications. Only, Amycolatopsis sp. GLM-2 showed tolerance to benomyl at the recommended dose. All the obtained actinobacteria were characterized as plant growth promoting strains by improving the growth of chili plants (Capsicum flutescens L.). Moreover, the co-inoculation treatment of the obtained actinobacteria and AMF (Claroideoglomus etunicatum) spores could significantly increase plant growth, contribute to the chlorophyll index, and enhance fruit production in chili plants. Additionally, the highest value of AMF spore production and the greatest percentage of root colonization were observed in the treatment that had been co-inoculated with Streptomyces sp. GETU-1.

scholarly journals Exploration of Rhizobacteria as Bioagents against Phytophthora Blight and Yield Attributes of Pigeonpea (Cajanus cajan L.): In vitro and In vivo Study

2021 ◽  
pp. 25-33
Author(s):  
Saroj Bala ◽  
Rajni Devi ◽  
Veena Khanna
Keyword(s):  
Plant Growth ◽  
Cajanus Cajan ◽  
Biochemical Characterization ◽  
Growth Parameters ◽  
Pigeon Pea ◽  
Yield Attributes ◽  
Plant Growth Promoting ◽  
Growth Promoting

The latest soil management scenario is occupied by destructive chemical fertilizers, which is a serious risk to both human health as well as to the environment. Advantageous microbes present in soil are used as a biofertilizers for a promising role in sustainable agriculture. Pigeon pea (Cajanus cajan L.) is a primitive protein rich leguminous pulse in India. Thirty-five isolates from rhizospheric soil samples were collected from twelve different locations of Punjab (India). Morphological and biochemical characterization for selection of potential plant growth promoting traits with antifungal properties was undertaken. Most of the inoculated seeds with rhizoisolates evolved a significant increase in growth parameters of pigeon pea as compared to uninoculated seeds, both in vitro and in vivo conditions. Plant growth promoting rhizobacterias (PGPRs) are environmentally safe as they lead to increased production and resistance against diseases of crops.

scholarly journals Impact of plant growth-promoting rhizobacteria on yield and disease control of Nicotiana tabacum

2018 ◽  
Vol 70 (4) ◽  
pp. 717-725
Author(s):  
Sohail Khan ◽  
Fazli Subhan ◽  
Kashif Haleem ◽  
Muhammad Khattak ◽  
Ibrar Khan ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Vitro Assay ◽  
Soil Microbial ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

An unexplored soil microbial community associated with the root system of Nicotiana tabacum was isolated to analyze its impact on growth and yield of the crop. A total of nine isolates out of 180 were biochemically screened and characterized as potential plant growth-promoting rhizobacteria due to the expression of growth-promoting traits. All isolates were positive for ammonia production, 8 were positive for phosphate solubilization but none for auxin production. The majority of the isolates were also found positive for hydrogen cyanide, siderophore and hydrolytic/degradative enzymes production, enabling them to restrict the growth of Fusarium oxysporum in an in vitro assay. Although all tested isolates enhanced tobacco growth significantly, Baj-ER-01 and CD-RS-03 were found to be the most promising in enhancing all aspects of growth. This study provides evidence for the enhancement of growth and yield of inoculated tobacco plants through an adequate supply of nutrients and/or controlling phytopathogens.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

2021 ◽  
Vol 9 (8) ◽  
pp. 1588
Author(s):  
Anastasia Venieraki ◽  
Styliani N. Chorianopoulou ◽  
Panagiotis Katinakis ◽  
Dimitris L. Bouranis
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Calcareous Soil ◽  
In Vitro Studies ◽  
Pgp Traits ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Microbial Fertilizers

Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

scholarly journals Plant Growth Promotion and Biocontrol Properties of Aneurinibacillus migulanus Isolated from Maize Roots (Zea mays)

2021 ◽  
pp. 46-59
Author(s):  
Lynda Kelvin Asogwa ◽  
Frank C. Ogbo
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Plant Height ◽  
Pot Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Maize Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020. Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot  and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods. Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment. Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting  properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.

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