Circulating Serum Levels of Fox P3, GATA-3 and IL-17 A as Potential Biomarkers in Patients with Symptomatic Asthma

2019 ◽  
Vol 51 (02) ◽  
pp. 28-37
Author(s):  
Ved Prakash ◽  
Keyword(s):  
Serum Levels ◽  
Symptomatic Asthma ◽  
Potential Biomarkers

scholarly journals Circulating miRNA-181b-5p, miRNA-223-3p, miRNA-210-3p, let 7i-5p, miRNA-21-5p and miRNA-29a-3p in patients with localized scleroderma as potential biomarkers

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Katarzyna Wolska-Gawron ◽  
Joanna Bartosińska ◽  
Marta Rusek ◽  
Małgorzata Kowal ◽  
Dorota Raczkiewicz ◽  
...  
Keyword(s):  
Longitudinal Research ◽  
Clinical Symptoms ◽  
Serum Levels ◽  
Surfactant Protein ◽  
Localized Scleroderma ◽  
Surfactant Protein D ◽  
Chemokine Ligand ◽  
Dipeptidylpeptidase 4 ◽  
Potential Biomarkers

AbstractLocalized scleroderma (LoSc) is a rare disease manifested by an inflammation and sclerosis of the skin. The latest studies focused on glycoprotein Krebs von den Lungen-6, surfactant protein-D, chemokine ligand 18 and dipeptidylpeptidase 4 as potential biomarkers of skin fibrosis in systemic scleroderma. Our study aimed to identify 6 miRNAs with elevated or decreased levels in 38 LoSc patients (31 females, 7 males) compared to healthy volunteers (HVs) and to correlate the selected miRNAs’ serum levels with the severity and the clinical symptoms of LoSc and some laboratory parameters with the selected miRNAs’ serum levels. The serum levels of miRNAs, i.e. miRNA-181b-5p, miRNA-223-3p, miRNA-21-5p, let 7i-5p, miRNA-29a-3p and miRNA-210-3p were significantly increased in the LoSc patients compared to the HVs. The level of let-7i increase in the female LoSc patients correlated negatively with BSA (r =  − 0.355, p = 0.049) and mLoSSI (r =  − 0.432, p = 0.015). Moreover, the female patients with inactive LoSc had significantly higher level of let-7i (2.68-fold on average) in comparison to those with active disease (p = 0.045). The exact role of those molecules has not been revealed in LoSc and a long-term longitudinal research is pivotal to confirm their prognostic value.

Biomarkers in vesicoureteral reflux: an overview

2020 ◽  
Vol 14 (8) ◽  
pp. 683-696
Author(s):  
Flávia C Valério ◽  
Renata D Lemos ◽  
Ana L de C Reis ◽  
Letícia P Pimenta ◽  
Érica LM Vieira ◽  
...  
Keyword(s):  
Vesicoureteral Reflux ◽  
Renal Scarring ◽  
Reflux Nephropathy ◽  
Serum Levels ◽  
Cellular Adhesion ◽  
High Grade ◽  
Epigenetic Factors ◽  
Cellular Adhesion Molecules ◽  
Potential Biomarkers

Aim: This article aimed to review the role of cytokines, chemokines, growth factors and cellular adhesion molecules as biomarkers for vesicoureteral reflux (VUR) and reflux nephropathy (RN). Methods: We reviewed articles from 1979 onward by searching PubMed and Scopus utilizing the combination of words: ‘VUR’ or ‘RN’ and each one of the biomarkers. Results: Genetic, inflammatory, fibrogenic, environmental and epigenetic factors responsible for renal scarring need to be better understood. TGF-β, IL-10, IL-6, IL-8 and TNF seem to exert a role in VUR particularly in RN based on the current literature. Serum levels of procalcitonin have been also associated with high-grade VUR and RN. These molecules should be more intensively evaluated as potential biomarkers for renal scarring in VUR. Conclusion: Further studies are necessary to define which molecules will really be of utility in clinical decisions and as therapeutic targets for VUR and RN.

scholarly journals Galectin-3, osteopontin and successful aging

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Fabian Sanchis-Gomar ◽  
Alejandro Santos-Lozano ◽  
Helios Pareja-Galeano ◽  
Nuria Garatachea ◽  
Rafael Alis ◽  
...  
Keyword(s):  
Successful Aging ◽  
Area Under The Curve ◽  
Predictor Variable ◽  
Serum Levels ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Galectin 3 ◽  
Medical Interest ◽  
Potential Use ◽  
Potential Biomarkers

AbstractBackground:Individuals who reach exceptional longevity (100+ years of age) free of common chronic age diseases (i.e. ‘dodgers’) arguably represent the paradigm of successful aging in humans. As such, identification of potential biomarkers associated with this phenomenon is of medical interest.Methods:We measured serum levels of galectin-3 and osteopontin, both of which have been shown to be linked with major chronic or aging-related disorders in younger populations, in centenarian ‘dodgers’ (n=81; 40 men; 100–104 years) and healthy controls (n=41; 24 men, 70–80 years).Results:Both biomarkers showed significantly lower values (p<0.001) in the former (galectin-3: 2.4±1.7 vs. 4.8±2.8 ng/mL; osteopontin: 38.1±27.7 vs. 72.6±33.1 μg/mL). Logistic regression analysis identified the combination of these two biomarkers as a significant predictor variable associated with successful aging regardless of sex (p<0.001). The area under the curve (AUC) classified the ability of galectin-3 and osteopontin to predict the likelihood of successful aging as ‘fair’ (AUC=0.75) and ‘good’ (AUC=0.80), respectively. Particularly, the combination of the two biomarkers showed good discriminatory power for successful aging (AUC=0.86), with sensitivity=83% and specificity=74%.Conclusions:Lower levels of both galectin-3 and osteopontin are associated with successful aging, representing potential biomarkers of this condition. Our cross-sectional data must be however approached with caution. Further research is necessary to replicate the present preliminary results in other cohorts and to identify the potential use of galectin-3 and osteopontin as potential targets (or at least predictors) in future personalized anti-aging therapies.

scholarly journals Calprotectin and Calgranulin C as Biomarkers of Pancreatic Tumors: Baseline Levels and Level Changes after Surgery

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Michal Holub ◽  
Eva Bartáková ◽  
Alžběta Stráníková ◽  
Eva Koblihová ◽  
Simona Arientová ◽  
...  
Keyword(s):  
Postoperative Period ◽  
Malignant Tumors ◽  
Serum Levels ◽  
Pancreatic Tissue ◽  
Pancreatic Tumors ◽  
Major Surgery ◽  
Benign Tumors ◽  
Baseline Serum ◽  
Predictive Values ◽  
Potential Biomarkers

Pancreatic tumors and their surgical resection are associated with significant morbidity and mortality, and the biomarkers currently used for these conditions have limited sensitivity and specificity. Because calprotectin and calgranulin C serum levels have been demonstrated to be potential biomarkers of certain cancers and complications of major surgery, the levels of both proteins were tested in the current study in patients with benign and malignant pancreatic tumors that were surgically removed. The baseline serum levels and kinetics of calprotectin and calgranulin C during the 7-day postoperative period were evaluated with immunoassays in 98 adult patients who underwent pancreatic surgery. The baseline serum levels of calprotectin and calgranulin C in patients with malignant (n=84) and benign tumors (n=14) were significantly higher (p<0.01) when compared to those in the healthy controls (n=26). The serum levels of both proteins were also significantly (p<0.05) higher in patients with benign tumors than in those with malignant tumors. After surgery, the serum levels of calprotectin and calgranulin C were significantly (p<0.01) higher than their baseline values, and this elevation persisted throughout the seven days of the follow-up period. Interestingly, starting on day 1 of the postoperative period, the serum levels of both proteins were significantly (p<0.05) higher in the 37 patients who developed postoperative pancreatic fistulas (POPFs) than in the patients who had uneventful recoveries (n=61). Moreover, the serum levels of calprotectin and calgranulin C demonstrated a significant predictive value for the development of POPF; the predictive values of these two proteins were better than those of the serum level of C-reactive protein and the white blood cell count. Taken together, the results of this study suggest that calprotectin and calgranulin C serum levels are potential biomarkers for pancreatic tumors, surgical injury to the pancreatic tissue and the development of POPFs.

scholarly journals Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Tong-ling Yuan ◽  
Jin Chen ◽  
Yan-li Tong ◽  
Yan Zhang ◽  
Yuan-yuan Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Bone Metabolism ◽  
Bone Remodeling ◽  
Heme Oxygenase ◽  
Serum Levels ◽  
Heme Oxygenase 1 ◽  
Tartrate Resistant Acid Phosphatase ◽  
Trap 5B ◽  
Potential Biomarkers

Backgrounds. Heme oxygenase-1 (HO-1) has been reported to play a regulatory role in osteoclastogenesis. Bone morphogenetic protein (BMP) pathways induce osteoblastic differentiation and bone remodeling.Aims. To identify serum levels of HO-1, BMP-7, and Runt related-transcription factor 2 (Runx2) in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS) and to investigate the relationships between HO-1, BMP-7, Runx2, and other common biomarkers for bone metabolism.Results. Serum levels of HO-1 and BMP-7 were revealed to be significantly higher in patients with RA or AS than in healthy controls (p<0.01). In RA group, HO-1 was positively correlated with BMP-7, Runx2, and tartrate-resistant acid phosphatase-5b (TRAP-5b) (p<0.05, resp.), BMP-7 was positively correlated with Runx2 and TRAP-5b (p<0.05, resp.), and Runx2 was negatively correlated with N-terminal midfragment of osteocalcin (NMID) (p<0.05). In AS group, we observed identical correlation between HO-1 and BMP-7, but opposite correlations between BMP-7 and TRAP-5b and between Runx2 and NMID, when comparing with the RA cohort.Conclusion. Our findings suggest that HO-1 and BMP-7 are potential biomarkers for bone metabolism in patients with RA and AS. The different correlations between the bone markers point to distinct differences in bone remodeling pathways in the two types of arthritis.

scholarly journals Evaluation of Serum Levels of Osteopontin and IgG Anti-Osteopontin Autoantibodies As Potential Biomarkers of Immune Activation in Patients with Allergic Diseases

2016 ◽  
Vol 137 (2) ◽  
pp. AB394
Author(s):  
Elisa Villa ◽  
Rosalba Minisini ◽  
Olaf Rötzschke ◽  
Kia Joo Puan ◽  
Boris Buenbrazo San Luis ◽  
...  
Keyword(s):  
Immune Activation ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Potential Biomarkers

scholarly journals Association of Matrix Metalloproteinases with Coronary Artery Calcification in Patients with CHD

2021 ◽  
Vol 11 (6) ◽  
pp. 506
Author(s):  
Yana V. Polonskaya ◽  
Elena V. Kashtanova ◽  
Ivan S. Murashov ◽  
Evgenia V. Striukova ◽  
Alexey V. Kurguzov ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Relative Risk ◽  
Matrix Metalloproteinases ◽  
Coronary Arteries ◽  
Serum Levels ◽  
Blood Levels ◽  
Relationship Of ◽  
The Relationship ◽  
Potential Biomarkers

This work is aimed at studying the relationship of matrix metalloproteinases with calcification of the coronary arteries. The study included 78 people with coronary heart disease (CHD) and 36 without CHD. Blood and samples of coronary arteries obtained as a result of endarterectomy were examined. Serum levels of metalloproteinases (MMP) MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-12, and MMP-13 were determined by multiplex analysis. In blood vessel samples, MMP-1, MMP-3, MMP-7, and MMP-9 were determined by enzyme immunoassay; MMP-9 expression was evaluated by immunohistochemistry. Patients with CHD had higher serum levels of MMP-1, MMP-7, and MMP-12. Blood levels of MMP-1 and MMP-3 were associated with calcium levels, MMP-9 with osteoprotegerin and osteonectin, MMP-7 and MMP-10 with osteoprotegerin, MMP-12 with osteocalcin, and MMP-13 with osteopontin. Calcified plaques had higher levels of MMP-1 and MMP-9 compared to plaques without calcification. The relative risk of coronary arteries calcification was associated with MMP-9, which is confirmed by the results of immunohistochemistry. The results obtained indicate the participation of some MMPs, and especially MMP-9, in the calcification processes. The study can serve as a basis for the further study of the possibility of using MMP-1, MMP-7 and MMP-12 as potential biomarkers of CHD.

scholarly journals Exosome-associated Mitochondrial DNA is Elevated in Patients with ME/CFS and Stimulates Human Cultured Microglia to Secrete IL-1β

2021 ◽  
Author(s):  
Theoharis C Theoharides ◽  
Irene Tsilioni ◽  
Benjamin Natelson
Keyword(s):  
Mitochondrial Dna ◽  
Sleep Disturbances ◽  
Serum Levels ◽  
Chronic Fatigue ◽  
Human Microglia ◽  
Fatigue Syndrome ◽  
Before And After ◽  
Pathogenetic Factor ◽  
Cognitive Problems ◽  
Potential Biomarkers

Abstract Background: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease that presents with fatigue, sleep disturbances, malaise and cognitive problems. The pathogenesis of ME/CFS is presently unknown and serum levels of potential biomarkers have been inconsistent. Methods: Exosomes were purified from serum obtained from patients with ME/CFS before and after exercise and their content of mitochondrial DNA (mtDNA) was determined by quantitative PCR. Exosomes from both patients and controls were incubated with cultured human microglia and release of interleukin-1beta (IL-1β) was measured by ELISA.Results: Here we show that serum mtDNA, associated with exosomes, is increased in ME/CFS after exercise. Moreover, exosomes isolated from patients with ME/CFS stimulate significant secretion of IL-1β from cultured human microglia. Conclusion: These results provide evidence for a potential novel pathogenetic factor and target for treatment of ME/CFS.

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