scholarly journals Iğdır’da Doğal Olarak Yetişen İğdelerin (Elaeagnus angustifolia L.) Seleksiyonu

2021 ◽  
Vol 7 (3) ◽  
pp. 353-359
Author(s):  
Kasım DUMAN ◽  
Mücahit PEHLUVAN ◽  
Rafet ASLANTAŞ ◽  
Ersin GÜLSOY
Keyword(s):  
Elaeagnus Angustifolia

Elaeagnus Angustifolia: a Promising Medicinal Plant for Cancer Theraby

2020 ◽  
Author(s):  
Islam Mohamed ◽  
Ahmed Moahmed ◽  
Mennatallah Abdelkader ◽  
Alaaeldin Saleh ◽  
Ala-Eddin Al-Moustafa
Keyword(s):  
Cell Proliferation ◽  
Oral Cancer ◽  
Medicinal Plant ◽  
Cancer Progression ◽  
Plant Extract ◽  
Cell Invasion ◽  
Elaeagnus Angustifolia ◽  
Flower Extract ◽  
Inhibition Of Angiogenesis ◽  
E Cadherin

Introduction: Elaeagnus angustifolia (EA) is a medicinal plant that has been used for centuries in treating many human diseases, in the Middle East, including fever, amoebic dysentery, gastrointestinal problems. However, the effect of EA plant extract on human cancer progression especially oral malignancy has not been investigated yet. Thus, first we examined the effect of EA flower extract on angiogenesis in ovo, and on selected parameters in human oral cancer cells. Materials and methods: Chorioallantoic membranes (CAMs) of chicken embryos at 3-7 days of incubation were used to assess the effect EAflower plant extract on angiogenesis. Meanwhile, cell proliferation, soft agar, cell cycle, cell invasion and cell wounding assays were performed to explore the outcome of EA plant extract on FaDu and SCC25 oral cancer cell lines. On the other hand, western blot analysis was carried out to evaluate E-cadherin and Erk1/Erk2 expression and activation, respectively, in FaDu and SCC25 under the effect of EA extract. Results: Our data show that EA extract inhibits cell proliferation and colony formation, in addition to the initiation of Scell cycle arrest and reductionof G1/G2 phases. In parallel, EA extract provokes differentiation to an epithelial phenotype “mesenchymal-epithelial transition: MET” which is the opposite of “epithelial-mesenchymal transition, EMT”: an important event in cell invasion and metastasis. Thus, EA extract causes a dramatic decrease in cell motility and invasion abilities of FaDu and SCC25 cancer cells in comparison with their controls. These changes are accompanied by an up-regulation of E-cadherin expression. The molecular pathway analysis of the EA flower extract reveals that it can inhibit the phosphorylation of Erk1/Erk2, which could be behind the inhibition of angiogenesis, the initiation of MET event and the overexpression of E-cadherin. Conclusions: Our findings indicate that EA plant extract can downgrade human oral cancer progression by the inhibition of angiogenesis and cell invasion via Erk1/Erk2 signaling pathways.

Phytochemistry and Phytotherapeutic Aspects of Elaeagnus angustifolia L.

2016 ◽  
Vol 13 (4) ◽  
pp. 199-210 ◽  
Author(s):  
Farid Niknam ◽  
Amir Azadi ◽  
Alireza Barzegar ◽  
Pouya Faridi ◽  
Nader Tanideh ◽  
...  
Keyword(s):  
Elaeagnus Angustifolia

Micropropagation of Elaeagnus angustifolia from mature trees

1995 ◽  
Vol 15 (10) ◽  
pp. 691-693 ◽  
Author(s):  
J. M. Iriondo ◽  
M. de la Iglesia ◽  
C. Perez
Keyword(s):  
Elaeagnus Angustifolia ◽  
Mature Trees

scholarly journals Microsatellite Markers for Russian Olive (Elaeagnus angustifolia; Elaeagnaceae)

2013 ◽  
Vol 1 (9) ◽  
pp. 1300013 ◽  
Author(s):  
John F. Gaskin ◽  
Ruth A. Hufbauer ◽  
Steven M. Bogdanowicz
Keyword(s):  
Microsatellite Markers ◽  
Elaeagnus Angustifolia ◽  
Russian Olive

Antinociceptive effect of Elaeagnus angustifolia fruit seeds in mice

Fitoterapia ◽  
2001 ◽  
Vol 72 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Mohammad Ramezani ◽  
Hossein Hosseinzadeh ◽  
Nosratollah Daneshmand
Keyword(s):  
Antinociceptive Effect ◽  
Elaeagnus Angustifolia

Investigating the Anti-Angiogenic Effects of Elaeagnus angustifolia L. Extract in Vivo

2021 ◽  
Vol 08 ◽  
Author(s):  
Mohammad Sako ◽  
Malek Zihlif ◽  
Fatma Afifi
Keyword(s):  
Blood Vessels ◽  
Cell Line ◽  
A549 Cells ◽  
Water Extract ◽  
Elaeagnus Angustifolia ◽  
Trypan Blue Exclusion ◽  
Antiangiogenic Effect ◽  
Proliferative Effect ◽  
Fgf2 Expression

Background: Angiogenesis is the formation of new blood vessels from pre-existing ones. It occurs in both physiological and pathological conditions. Objectives: We aimed to evaluate the antiangiogenic effect of Elaeagnus angustifolia L. water extract in vivo to determine any anti-proliferative effect of the extract on the A549 lung cancer cell line, and to investigate its effect on VEGF-A and FGF2 expression in the A549 cell line. Methods: Trypan blue exclusion test was implemented to establish any possible anti-proliferative effect of the extract. Then, Matrigel plug assay was performed on mice using the same cell line to test the antiangiogenic effect of the extract. Finally, A549 cells were treated with the extract at concentrations of 25, 12.5, and 6.25 µg/ml to investigate the changes in VEGF-A and FGF2 expression by RT-qPCR. Results: E. angustifolia extract did not exhibit a significant anti-proliferative effect against A549 cells. The extract at concentrations of 12.5 and 6.25 µg/ml demonstrated an inhibitory effect against the growth of new blood vessels by 75.63 and 45.26%, respectively. The extract did not affect the expression of VEGF-A and FGF2 in A549 cells. Conclusion: Our findings show that water extract of E. angustifolia possesses potent antiangiogenic activity, while neither exhibiting significant anti-proliferative effect nor affecting VEGF-A or FGF2 expression in the A459 cell line, suggesting either sole direct antiangiogenic effect, or both direct and indirect effects with paracrine suppression of other genes.

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